SCI和EI收录∣中国化工学会会刊

中国化学工程学报 ›› 2021, Vol. 38 ›› Issue (10): 205-215.DOI: 10.1016/j.cjche.2021.03.022

• Biotechnology and Bioengineering • 上一篇    下一篇

Development of genipin crosslinked gelatin matrices on surface interaction: Enhancing the biocompatibility by attenuating sterile inflammation

Qiang-Song Wang1, Gui-Fang Wang2, Hai-Yun Zhang2, Fa-Quan Zhao2, Yuan-Lu Cui2   

  1. 1. Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192, China;
    2. State Key Laboratory of Component-based Chinese Medicine, Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
  • 收稿日期:2020-12-28 修回日期:2021-02-27 出版日期:2021-10-28 发布日期:2021-12-02
  • 通讯作者: Yuan-Lu Cui
  • 基金资助:
    This work was supported by the National Natural Science Foundation of China (81741119) and Fundamental Research Funds for the Central Universities, China (3332020059).

Development of genipin crosslinked gelatin matrices on surface interaction: Enhancing the biocompatibility by attenuating sterile inflammation

Qiang-Song Wang1, Gui-Fang Wang2, Hai-Yun Zhang2, Fa-Quan Zhao2, Yuan-Lu Cui2   

  1. 1. Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192, China;
    2. State Key Laboratory of Component-based Chinese Medicine, Research Center of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
  • Received:2020-12-28 Revised:2021-02-27 Online:2021-10-28 Published:2021-12-02
  • Contact: Yuan-Lu Cui
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (81741119) and Fundamental Research Funds for the Central Universities, China (3332020059).

摘要: The inflammation can be stimulated by the surgical implantation and biomaterial presence through the foreign body via bio-interface. Macrophages play a key role in the interaction of host tissue to implant surfaces. In present study, the immuno-inflammatory responses of genipin crosslinked gelatin matrices (GCGM) to macrophages in vitro and the host tissue surfaces in rats were investigated. The results showed that the mechanical properties, swelling and degradation of gelatin matrices were improved by the crosslinking of genipin at physiological conditions. The macrophage on the surface of GCGM could avoid to be activated. The interaction of macrophage and GCGM suggested that GCGM could reduce the inflammatory response with downregulating the production and mRNA expression of pro-inflammatory cytokines. The anti-inflammatory effect of GCGM was demonstrated to be related to inhibit nuclear factor kappa-B (NF-κB) signaling pathway. Furthermore, gelatin matrices crosslinked with genipin could decrease the acute and chronic inflammatory response interacting with host tissue surfaces to enhance the biocompatibility in rats. These results showed that GCGM could avoid to active macrophages and were endowed with anti-inflammatory properties, suggesting the significant potential for clinical success with the development of immunomodulatory biomaterials.

关键词: Genipin, Gelatin matrices, Anti-inflammation, Biocompatibility, NF-κB

Abstract: The inflammation can be stimulated by the surgical implantation and biomaterial presence through the foreign body via bio-interface. Macrophages play a key role in the interaction of host tissue to implant surfaces. In present study, the immuno-inflammatory responses of genipin crosslinked gelatin matrices (GCGM) to macrophages in vitro and the host tissue surfaces in rats were investigated. The results showed that the mechanical properties, swelling and degradation of gelatin matrices were improved by the crosslinking of genipin at physiological conditions. The macrophage on the surface of GCGM could avoid to be activated. The interaction of macrophage and GCGM suggested that GCGM could reduce the inflammatory response with downregulating the production and mRNA expression of pro-inflammatory cytokines. The anti-inflammatory effect of GCGM was demonstrated to be related to inhibit nuclear factor kappa-B (NF-κB) signaling pathway. Furthermore, gelatin matrices crosslinked with genipin could decrease the acute and chronic inflammatory response interacting with host tissue surfaces to enhance the biocompatibility in rats. These results showed that GCGM could avoid to active macrophages and were endowed with anti-inflammatory properties, suggesting the significant potential for clinical success with the development of immunomodulatory biomaterials.

Key words: Genipin, Gelatin matrices, Anti-inflammation, Biocompatibility, NF-κB