SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2020, Vol. 28 ›› Issue (4): 1136-1144.doi: 10.1016/j.cjche.2019.12.025

• Biotechnology and Bioengineering • Previous Articles     Next Articles

Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation

Jelena Bebić1, Katarina Banjanac1,2, Marija Ćorović3, Ana Milivojević2, Milica Simović3, Aleksandar Marinković4, Dejan Bezbradica3   

  1. 1 Directorate of Measures and Precious Metals, Mike Alasa 14, 11000 Belgrade, Serbia;
    2 Innovation Centre of Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia;
    3 Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia;
    4 Department of Organic Chemistry, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia
  • Received:2019-09-28 Revised:2019-12-19 Online:2020-04-28 Published:2020-07-27
  • Contact: Jelena Bebić, Katarina Banjanac, Marija Ćorović, Ana Milivojević, Milica Simović, Aleksandar Marinković, Dejan Bezbradica E-mail:jelenabebic@dmdm.rs;kbanjanac@tmf.bg.ac.rs;mstojanovic@tmf.bg.ac.rs;amilivojevic@tmf.bg.ac.rs;mcarevic@tmf.bg.ac.rs;marinko@tmf.bg.ac.rs;dbez@tmf.bg.ac.rs
  • Supported by:
    Authors are grateful to the Ministry of Education, Science and Technological Development, Republic of Serbia, within projects Ⅲ 46010, Ⅲ 45019 and TR31035 for the financial, and to Directorate of Measures and Precious Metals, Ministry of Economy, Republic of Serbia for the technical support. We thank Dr. Pavle Andrić (Novozymes A/S, Bagsvaerd, Denmark) for providing commercial laccase from M. thermophila expressed in A. oryzae (Novozym® 51003).

Abstract: This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oryzae (Novozym 51003® laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003® laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU·mg-1 proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity.

Key words: Silica nanoparticles, Laccase, Enzyme immobilization, Lindane, Bioremediation