Chinese Journal of Chemical Engineering ›› 2021, Vol. 29 ›› Issue (2): 204-211.doi: 10.1016/j.cjche.2020.12.008

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Raman spectroscopy as process analytical tool in downstream processing of biotechnology

Yu Kiat Lin1, Hui Yi Leong1, Tau Chuan Ling2, Dong-Qiang Lin1, Shan-Jing Yao1   

  1. 1 Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
    2 Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia
  • Received:2020-10-13 Revised:2020-12-04 Online:2021-02-28 Published:2021-05-15
  • Contact: Shan-Jing Yao
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (No. 21878263, 22078286). Dr. Yu Kiat Lin would like to thank Zhejiang University and Talent-Introduction Program of China for Postdoctoral Researcher for the financial support.

Abstract: Downstream processing or product recovery plays a vital role in the development of bioprocesses. To improve the bioprocess efficiency, some unconventional methods are much required. The continuous manufacturing in downstream processing makes the Process Analytical Technologies (PATs) as an important tool. Monitoring and controlling bioprocess are an essential factor for the principles of PAT and quality by design. Spectroscopic methods can apply to monitor multiple analytes in real-time with less sample processing with significant advancements. Raman spectroscopy is an extensively used technique as an analytical and research tool owing to its modest process form, non-destructive, non-invasive optical molecular spectroscopic imaging with computer-based analysis. Generally, its application is essential for the analysis and characterization of biological samples, and it is easy to operate with minimal sample. The innovation on various types of enhanced Raman spectroscopy was designed to enhance the Raman analytical technique. Raman spectroscopy could couple with chemometrics to provide reliable alternative analysis method of downstream process analysis. Thus, this review aims to provide useful insight on the application of Raman spectroscopy for PAT in downstream processing of biotechnology and Raman data analysis in biological fields.

Key words: Raman spectroscopy, Process analytical technology, Downstream bioprocessing, Biotechnology