SCI和EI收录∣中国化工学会会刊

›› 2011, Vol. 19 ›› Issue (5): 876-880.

• SELECTED PAPERS FROM THE 4TH NATIONAL CONFERENCE ON MASS TRANSFER AND SEPARATION ENGINEERING AND IN HONOUR OF PROF. K. T. YU (YU GUOCONG) • Previous Articles     Next Articles

Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel

YAN Luding, SHEN Shaochuan, YUN Junxian, YAO Kejian   

  1. State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China
  • Received:2010-01-21 Revised:2011-04-20 Online:2011-10-28 Published:2011-10-28
  • Supported by:
    Supported by the National Natural Science Foundation of China (21036005, 20876145);the Science and Technology Cooperation Project between China-Europe Country’s Governments from the Ministry of Science and Technology of China (1017);the Natural Science Foundation of Zhejiang Provincial (Y4080326)

Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel

晏禄丁, 沈绍传, 贠军贤, 姚克俭   

  1. State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China
  • 通讯作者: YAO Kejian,E-mail:yaokj@zjut.edu.cn
  • 基金资助:
    Supported by the National Natural Science Foundation of China (21036005, 20876145);the Science and Technology Cooperation Project between China-Europe Country’s Governments from the Ministry of Science and Technology of China (1017);the Natural Science Foundation of Zhejiang Provincial (Y4080326)

Abstract: An effective cation-exchange chromatographic method for lysozyme isolation from chicken egg white is presented, using supermacroporous cryogel grafted with sulfo functional groups. The chromatographic processes were carried out by one-step and sequential elution, respectively. Sodium phosphate buffer (pH 7.8) containing different concentrations of NaCl is used as elution agent. The corresponding breakthrough characteristics and elution behaviors in the cryogel bed were investigated and analyzed. Purity of lysozyme in the elution effluent was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The maximum purity of the obtained lysozyme was about 96%, and the cryogel is demonstrated as a potential separation medium for purification of high-purity lysozyme from chicken egg white.

Key words: cation-exchange cryogel, lysozyme, isolation, sequential elution, chicken egg white

摘要: An effective cation-exchange chromatographic method for lysozyme isolation from chicken egg white is presented, using supermacroporous cryogel grafted with sulfo functional groups. The chromatographic processes were carried out by one-step and sequential elution, respectively. Sodium phosphate buffer (pH 7.8) containing different concentrations of NaCl is used as elution agent. The corresponding breakthrough characteristics and elution behaviors in the cryogel bed were investigated and analyzed. Purity of lysozyme in the elution effluent was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The maximum purity of the obtained lysozyme was about 96%, and the cryogel is demonstrated as a potential separation medium for purification of high-purity lysozyme from chicken egg white.

关键词: cation-exchange cryogel, lysozyme, isolation, sequential elution, chicken egg white