%0 Journal Article %A Xianling Meng %A Xia Jiang %A Peijun Ji %T A strong adhesive block polymer coating for antifouling of large molecular weight protein %D 2017 %R 10.1016/j.cjche.2017.04.007 %J Chinese Journal of Chemical Engineering %P 1831-1837 %V 25 %N 12 %X Some proteins secreted by microorganisms have large molecularweights.Wereport here an approach to prepare coating by multilayer polymers for antifouling of proteins, especially the proteins with a large molecular weight. Stainless steel was used as the model substrate. The substrate was first coatedwith a hybrid polymer film, which was formed by simultaneous hydrolytic polycondensation of 3-aminopropyltriethoxysilane and polymerization of dopamine (HPAPD). After grafting the macroinitiator 2-bromoisobutyryl bromide, the block polymer brushes PMMA-b-PHEMA were grafted. Three proteins were used to test protein adsorption and antifouling behavior of the coating, including recombinant green fluorescent (54 kDa), recombinant R-transaminase (2×90 kDa), and recombinant catalase (4×98 kDa). It is demonstrated that the block polymer brushes not only can prevent the adsorption of small molecular weight proteins, but also can significantly reduce the adsorption of the large molecular weight proteins. %U https://cjche.cip.com.cn/EN/10.1016/j.cjche.2017.04.007