SCI和EI收录∣中国化工学会会刊

中国化学工程学报 ›› 2022, Vol. 44 ›› Issue (4): 1-7.DOI: 10.1016/j.cjche.2021.06.013

• •    下一篇

Photoacoustic detection of follicular thyroid carcinoma using targeted Nano-Au-Tripods

Yang Gui1, Kai Cheng2, Ruojiao Wang1, Sirui Liu1, Chenyang Zhao1, Rui Zhang1, Ming Wang1, Zhen Cheng2, Meng Yang1,2   

  1. 1 Department of Ultrasound, State Key Laboratory of Complex Severe and Rare Diseases, Pecking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China;
    2 Molecular Imaging Program at Stanford (MIPS), Department of Radiology and Bio-X Program, School of Medicine, Stanford University, Stanford CA 94305-5484, USA
  • 收稿日期:2021-02-07 修回日期:2021-06-03 出版日期:2022-04-28 发布日期:2022-06-18
  • 通讯作者: Zhen Cheng,E-mail:zcheng@stanford.edu;Meng Yang,E-mail:amengameng@hotmail.com
  • 基金资助:
    This work was supported by the National Natural Science Foundation of China (81421004, 81301268); Beijing Nova Program Interdisciplinary Cooperation Project xxjc201812); International S&T Cooperation Program of China (2015DFA30440); Beijing Nova Program (Z131107000413063); CAMS Innovation Fund for Medical Sciences (CIFMS 2020-I2M-C&T-B-035).

Photoacoustic detection of follicular thyroid carcinoma using targeted Nano-Au-Tripods

Yang Gui1, Kai Cheng2, Ruojiao Wang1, Sirui Liu1, Chenyang Zhao1, Rui Zhang1, Ming Wang1, Zhen Cheng2, Meng Yang1,2   

  1. 1 Department of Ultrasound, State Key Laboratory of Complex Severe and Rare Diseases, Pecking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China;
    2 Molecular Imaging Program at Stanford (MIPS), Department of Radiology and Bio-X Program, School of Medicine, Stanford University, Stanford CA 94305-5484, USA
  • Received:2021-02-07 Revised:2021-06-03 Online:2022-04-28 Published:2022-06-18
  • Contact: Zhen Cheng,E-mail:zcheng@stanford.edu;Meng Yang,E-mail:amengameng@hotmail.com
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (81421004, 81301268); Beijing Nova Program Interdisciplinary Cooperation Project xxjc201812); International S&T Cooperation Program of China (2015DFA30440); Beijing Nova Program (Z131107000413063); CAMS Innovation Fund for Medical Sciences (CIFMS 2020-I2M-C&T-B-035).

摘要: Follicular thyroid carcinoma (FTC) is the second most common form of thyroid malignancy, and it is associated with more aggressive growth and worse long-term survival outcomes relative to papillary thyroid carcinoma (PTC). Reliable approaches to preoperative FTC detection, however, remain to be established. Herein, a targeted Affibody-Au-Tripod nanoprobe was developed and successfully utilized to facilitate the targeted photoacoustic imaging (PAI) of epidermal growth factor receptor (EGFR)-positive cells and tumors. These Affibody-Au-Tripods were found to be highly sensitive and specific for cells expressing EGFR when used as a PA contrast agent in vitro, and studies conducted in an FTC-133 subcutaneous tumor model system in mice further revealed that these Affibody-Au-Tripods were able to specifically target these EGFR-expressing tumors while providing a strong photoacoustic signal in vivo. Importantly, these nanoprobes exhibited negligible cytotoxicity and robust chemical and physical stability, making Affibody-Au-Tripods promising candidates for targeted PAI-based FTC diagnosis. In addition, these nanoprobes have the potential to facilitate the individualized treatment of patients harboring EGFR-positive tumors.

关键词: Affibody, Follicular thyroid carcinoma, Nano-Au-Tripods, Nanoprobe, Photoacoustic imaging

Abstract: Follicular thyroid carcinoma (FTC) is the second most common form of thyroid malignancy, and it is associated with more aggressive growth and worse long-term survival outcomes relative to papillary thyroid carcinoma (PTC). Reliable approaches to preoperative FTC detection, however, remain to be established. Herein, a targeted Affibody-Au-Tripod nanoprobe was developed and successfully utilized to facilitate the targeted photoacoustic imaging (PAI) of epidermal growth factor receptor (EGFR)-positive cells and tumors. These Affibody-Au-Tripods were found to be highly sensitive and specific for cells expressing EGFR when used as a PA contrast agent in vitro, and studies conducted in an FTC-133 subcutaneous tumor model system in mice further revealed that these Affibody-Au-Tripods were able to specifically target these EGFR-expressing tumors while providing a strong photoacoustic signal in vivo. Importantly, these nanoprobes exhibited negligible cytotoxicity and robust chemical and physical stability, making Affibody-Au-Tripods promising candidates for targeted PAI-based FTC diagnosis. In addition, these nanoprobes have the potential to facilitate the individualized treatment of patients harboring EGFR-positive tumors.

Key words: Affibody, Follicular thyroid carcinoma, Nano-Au-Tripods, Nanoprobe, Photoacoustic imaging