SCI和EI收录∣中国化工学会会刊

Chin.J.Chem.Eng. ›› 2019, Vol. 27 ›› Issue (2): 418-425.DOI: 10.1016/j.cjche.2018.04.005

• Biotechnology and Bioengineering • Previous Articles     Next Articles

Optimizational production of phenyllactic acid by a Lactobacillus buchneri strain via uniform design with overlay sampling methodology

Jintao Guan1,2, Chaofei Han2, Yixin Guan1, Songhong Zhang2, Junxian Yun2, Shanjing Yao1   

  1. 1 College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
    2 State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310032, China
  • Received:2018-01-29 Revised:2018-03-15 Online:2019-03-18 Published:2019-02-28
  • Contact: Yixin Guan, Junxian Yun
  • Supported by:

    Supported partially by the National Natural Science Foundation of China (21576240) and the Natural Science Foundation of Zhejiang Province (LZ14B060001, LY16B060011)

Optimizational production of phenyllactic acid by a Lactobacillus buchneri strain via uniform design with overlay sampling methodology

Jintao Guan1,2, Chaofei Han2, Yixin Guan1, Songhong Zhang2, Junxian Yun2, Shanjing Yao1   

  1. 1 College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
    2 State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310032, China
  • 通讯作者: Yixin Guan, Junxian Yun
  • 基金资助:

    Supported partially by the National Natural Science Foundation of China (21576240) and the Natural Science Foundation of Zhejiang Province (LZ14B060001, LY16B060011)

Abstract: A Lactobacillus buchneri GBS3 strain isolated from the traditional Chinese pickles was used for the production of 3- phenyllactic acid (PLA), an important compound with antimicrobial activities against a wide species of grampositive and gram-negative bacteria and some fungi. The growth performance of this strain in the de Man, Rogosa and Sharpe (MRS) medium, the production of metabolites of valuable organic acids, and the biosynthesis of PLA using this strain as the whole-cell biocatalyst and phenylpyruvic acid (PPA) as the precursor, were investigated experimentally. The uniform design method with overlay sampling was developed for the optimization of the biotransformation conditions. The results showed that although it produced naturally lactic acid with the maximum concentration of 1.84 g·L-1 and PLA with the concentration of 0.015 g·L-1 after 66 to 72 h cultivation in MRS broth by fermentation, the present strain displayed an effective utilization ability by transforming PPA to PLA. By the uniform design method with overlay sampling for the design and optimization of transformation conditions, a maximum yield of 10.93 g·L-1 PLA with the mole conversion ratio of 83.07% from PPA to PLA was achieved under the optimized condition, i.e., 20 g·L-1 glucose, 270 g·L-1 cells, 13 g·L-1 PPA, pH 8.0 and the reaction time of 15 h, indicating that Lactobacillus buchneri GBS3 was an interesting strain for the biosynthesis of PLA via the microbial transformation. The prediction of PLA yield under different conditions was achieved successfully based on the limited information of only a small number of experiments by the uniform design with overlay sampling. Therefore, the present methodology is effective and helpful for the optimization of the biosynthesis processes of PLA.

Key words: Phenyllactic Acid, Biotransformation, Lactobacillus Buchneri, Uniform Design, Overlap Sampling

摘要: A Lactobacillus buchneri GBS3 strain isolated from the traditional Chinese pickles was used for the production of 3- phenyllactic acid (PLA), an important compound with antimicrobial activities against a wide species of grampositive and gram-negative bacteria and some fungi. The growth performance of this strain in the de Man, Rogosa and Sharpe (MRS) medium, the production of metabolites of valuable organic acids, and the biosynthesis of PLA using this strain as the whole-cell biocatalyst and phenylpyruvic acid (PPA) as the precursor, were investigated experimentally. The uniform design method with overlay sampling was developed for the optimization of the biotransformation conditions. The results showed that although it produced naturally lactic acid with the maximum concentration of 1.84 g·L-1 and PLA with the concentration of 0.015 g·L-1 after 66 to 72 h cultivation in MRS broth by fermentation, the present strain displayed an effective utilization ability by transforming PPA to PLA. By the uniform design method with overlay sampling for the design and optimization of transformation conditions, a maximum yield of 10.93 g·L-1 PLA with the mole conversion ratio of 83.07% from PPA to PLA was achieved under the optimized condition, i.e., 20 g·L-1 glucose, 270 g·L-1 cells, 13 g·L-1 PPA, pH 8.0 and the reaction time of 15 h, indicating that Lactobacillus buchneri GBS3 was an interesting strain for the biosynthesis of PLA via the microbial transformation. The prediction of PLA yield under different conditions was achieved successfully based on the limited information of only a small number of experiments by the uniform design with overlay sampling. Therefore, the present methodology is effective and helpful for the optimization of the biosynthesis processes of PLA.

关键词: Phenyllactic Acid, Biotransformation, Lactobacillus Buchneri, Uniform Design, Overlap Sampling