SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2020, Vol. 28 ›› Issue (8): 2028-2036.DOI: 10.1016/j.cjche.2020.04.010

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Microenvironmental engineering: An effective strategy for tailoring enzymatic activities

Yifei Zhang, Henry Hess   

  1. Department of Biomedical Engineering, Columbia University, 351 L Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA
  • Received:2020-01-25 Revised:2020-04-07 Online:2020-09-19 Published:2020-08-28
  • Contact: Yifei Zhang
  • Supported by:
    This work was supported by the National Science Foundation of USA under Award Number NSF-ENG 1844149.

Microenvironmental engineering: An effective strategy for tailoring enzymatic activities

Yifei Zhang, Henry Hess   

  1. Department of Biomedical Engineering, Columbia University, 351 L Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA
  • 通讯作者: Yifei Zhang
  • 基金资助:
    This work was supported by the National Science Foundation of USA under Award Number NSF-ENG 1844149.

Abstract: Rationally, engineering a favorable physicochemical microenvironment for enzymes has recently emerged as an effective strategy to improve their catalytic performance. In this review, we discuss four microenvironmental effects according to the mechanism of action: localizing and excluding reactants and regulators, regulating microenvironmental pH, creating a water-like microenvironment, and increasing the local temperature. These mechanisms are enzyme-independent and can in principle be used in combination to tailor enzyme behaviors, offering new approaches to enabling, enhancing, and regulating enzyme catalysis in diverse applications without the need for genetic engineering.

Key words: Biocatalysis, Enzyme, Microenvironment, Activity enhancement

摘要: Rationally, engineering a favorable physicochemical microenvironment for enzymes has recently emerged as an effective strategy to improve their catalytic performance. In this review, we discuss four microenvironmental effects according to the mechanism of action: localizing and excluding reactants and regulators, regulating microenvironmental pH, creating a water-like microenvironment, and increasing the local temperature. These mechanisms are enzyme-independent and can in principle be used in combination to tailor enzyme behaviors, offering new approaches to enabling, enhancing, and regulating enzyme catalysis in diverse applications without the need for genetic engineering.

关键词: Biocatalysis, Enzyme, Microenvironment, Activity enhancement