%0 Journal Article %A Jelena Bebić %A Katarina Banjanac %A Marija Ćorović %A Ana Milivojević %A Milica Simović %A Aleksandar Marinković %A Dejan Bezbradica %T Immobilization of laccase from Myceliophthora thermophila on functionalized silica nanoparticles: Optimization and application in lindane degradation %D 2020 %R 10.1016/j.cjche.2019.12.025 %J Chinese Journal of Chemical Engineering %P 1136-1144 %V 28 %N 4 %X This work is focused on immobilization of laccase from Myceliophthora thermophila expressed in Aspergillus oryzae (Novozym 51003® laccase) on amino modified fumed nano-silica (AFNS) and the possible use in bioremediation. Hereby, for the first time, factors affecting the immobilization of Novozym 51003® laccase on AFNS were investigated for defining the immobilization mechanism and optimizing the utilization of AFNS as support for laccase immobilization. The highest specific activity (13.1 IU·mg-1 proteins) was achieved at offered 160 mg per g of AFNS and for the same offered protein concentration the highest activity immobilization yield, reaching 68.3% after the equilibrium time, at optimum pH 5.0, was obtained. Laccase immobilization occurs by adsorption as monolayer enzyme binding in 40 min, following pseudo-first-order kinetics. The possible use of obtained immobilized preparation was investigated in degradation of pesticide lindane. Within 24 h, lindane concentration was reduced to 56.8% of initial concentration and after seven repeated reuses it retained 70% of the original activity. %U https://cjche.cip.com.cn/EN/10.1016/j.cjche.2019.12.025