Enhancing the Activity of Glutamate Decarboxylase from Lactobacillus brevis by Directed Evolution

doi:10.1016/j.cjche.2014.09.025

›› 2014, Vol. 22 ›› Issue (11/12): 1322-1327.DOI: 10.1016/j.cjche.2014.09.025

• BIOTECHNOLOGY AND BIOENGINEERING • Previous Articles Next Articles

Enhancing the Activity of Glutamate Decarboxylase from Lactobacillus brevis by Directed Evolution

Ling Lin¹, Sheng Hu², Kai Yu¹, Jun Huang³, Shanjing Yao¹, Yinlin Lei², Guixiang Hu², Lehe Mei^1,2

1 Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2 School of Biotechnology and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China;
3 School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China

Received:2013-06-04 Revised:2013-10-29 Online:2014-12-24 Published:2014-12-28
Supported by:
Supported by the National Natural Science Foundation of China (30970638, 21176220, and 31240054) and the National Natural Science Foundation of Zhejiang Province (LZ13B060002).

Enhancing the Activity of Glutamate Decarboxylase from Lactobacillus brevis by Directed Evolution

Ling Lin¹, Sheng Hu², Kai Yu¹, Jun Huang³, Shanjing Yao¹, Yinlin Lei², Guixiang Hu², Lehe Mei^1,2

1 Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
2 School of Biotechnology and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China;
3 School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China

通讯作者: Lehe Mei
基金资助:
Supported by the National Natural Science Foundation of China (30970638, 21176220, and 31240054) and the National Natural Science Foundation of Zhejiang Province (LZ13B060002).

Abstract

Abstract: Glutamate decarboxylase (GAD, EC4.1.1.15) can catalyze the decarboxylation of _L-glutamate to form γ- aminobutyrate (GABA), which is in great demand in some foods and pharmaceuticals. In our previous study, gad, the gene coding glutamate decarboxylase from Lactobacillus brevis CGMCC 1306, was cloned and its soluble expression was realized. In this study, error-prone PCR was conducted to improve its activity, followed by a screening. Mutant Q51H with high activity [55.4 mmol·L^-1·min^-1·(mg protein)^-1, 120% higher than that of the wild type at pH 4.8] was screened out from the mutant library. In order to investigate the potential role of this site in the regulation of enzymatic activity, site-directed saturation mutagenesis at site 51 was carried out, and three specific mutants, N-terminal truncated GAD, Q51P, and Q51L, were identified. The kinetic parameters of the three mutants and Q51Hwere characterized. The results reveal that aspartic acid at site 88 and N-terminal domain are essential to the activity aswell as correct folding of GAD. This study not only improves the activity of GAD, but also sheds new light on the structure-function relationship of GAD.

Key words: Directed evolution, Recombinant DNA, Mutagenesis, Enzyme activity, Kinetic parameter, Bio-synthesis

摘要： Glutamate decarboxylase (GAD, EC4.1.1.15) can catalyze the decarboxylation of _L-glutamate to form γ- aminobutyrate (GABA), which is in great demand in some foods and pharmaceuticals. In our previous study, gad, the gene coding glutamate decarboxylase from Lactobacillus brevis CGMCC 1306, was cloned and its soluble expression was realized. In this study, error-prone PCR was conducted to improve its activity, followed by a screening. Mutant Q51H with high activity [55.4 mmol·L^-1·min^-1·(mg protein)^-1, 120% higher than that of the wild type at pH 4.8] was screened out from the mutant library. In order to investigate the potential role of this site in the regulation of enzymatic activity, site-directed saturation mutagenesis at site 51 was carried out, and three specific mutants, N-terminal truncated GAD, Q51P, and Q51L, were identified. The kinetic parameters of the three mutants and Q51Hwere characterized. The results reveal that aspartic acid at site 88 and N-terminal domain are essential to the activity aswell as correct folding of GAD. This study not only improves the activity of GAD, but also sheds new light on the structure-function relationship of GAD.

关键词: Directed evolution, Recombinant DNA, Mutagenesis, Enzyme activity, Kinetic parameter, Bio-synthesis

Ling Lin, Sheng Hu, Kai Yu, Jun Huang, Shanjing Yao, Yinlin Lei, Guixiang Hu, Lehe Mei. Enhancing the Activity of Glutamate Decarboxylase from Lactobacillus brevis by Directed Evolution[J]. , 2014, 22(11/12): 1322-1327.

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Enhancing the Activity of Glutamate Decarboxylase from Lactobacillus brevis by Directed Evolution

Enhancing the Activity of Glutamate Decarboxylase from Lactobacillus brevis by Directed Evolution

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