Abstract: E.coli cells expressing recombinant human interferon-γwas disrupted by sonication anddissolved in 7mol·L~(-1) guanidine hydrochloride.The extract obtained was then renaturated by 70 folddilution with PBS.HulFN γwas purified by affinity chromatography with monoclonal antibody fromthe renaturated crude feed solution.After washing the column with PBS,the adsorbed HulFN γwaseluted with PBS containing 0.5mol·L~(-1) NaCl.The column was regenerated with 2mol·L~(-1) GuHClfor reuse.After one step of affinity purification the purity of interferon-γwas over 95%.and thespecific activity of the HulFN-γreached 1.2×10~7 IU·mg~(-1) protein.92.8% of recovery was obtainedin the elution step.Total recovery of HulFN γactivity in the affinity chromatography was 78%.

Key words: monoclonal antibody, affinity chromatographv, recombinant human interferon

摘要： E.coli cells expressing recombinant human interferon-γwas disrupted by sonication anddissolved in 7mol·L~(-1) guanidine hydrochloride.The extract obtained was then renaturated by 70 folddilution with PBS.HulFN γwas purified by affinity chromatography with monoclonal antibody fromthe renaturated crude feed solution.After washing the column with PBS,the adsorbed HulFN γwaseluted with PBS containing 0.5mol·L~(-1) NaCl.The column was regenerated with 2mol·L~(-1) GuHClfor reuse.After one step of affinity purification the purity of interferon-γwas over 95%.and thespecific activity of the HulFN-γreached 1.2×10~7 IU·mg~(-1) protein.92.8% of recovery was obtainedin the elution step.Total recovery of HulFN γactivity in the affinity chromatography was 78%.

关键词: monoclonal antibody;affinity chromatographv;recombinant human interferon