Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Recombinant Pseudomonas stutzeri 1317 from Unrelated Carbon Sources

doi:10.1016/S1004-9541(13)60549-2

Chin.J.Chem.Eng. ›› 2013, Vol. 21 ›› Issue (9): 1057-1061.DOI: 10.1016/S1004-9541(13)60549-2

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Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Recombinant Pseudomonas stutzeri 1317 from Unrelated Carbon Sources

WEI Xiaoxing^1,2, LIU Feng^2,3, JIAN Jia², WANG Ruiyan², CHEN Guoqiang^2,4

1 Department of Basic Medicine, Medical College of Qinghai University, Xining 810016, China;
2 MOE Key Lab of Bioinformatics and Systems Biology, Department of Biological Science and Biotechnology, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China;
3 Tuspark Kunshan Biotech Science Park Development Center, 4/F, Science & Technology Plaza, Tsinghua Science Park, Kunshan, Jiangsu 215347, China;
4 Center for Nano and Micro Mechanics, Tsinghua University, Beijing 100084, China

Received:2012-09-27 Revised:2013-02-28 Online:2013-10-01 Published:2013-09-28
Supported by:
Supported by the National Natural Science Foundation of China (31260015), Natural Science Foundation of Qinghai Province (2012-Z-919Q), the Extramural Project from State Key Laboratory for Agrobiotechnology (2012SKLAB06-5) and the Research Funds for Young Project of Qinghai University (2011-QYY-1)

Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Recombinant Pseudomonas stutzeri 1317 from Unrelated Carbon Sources

魏晓星^1,2, 刘峰^2,3, 简嘉², 王瑞妍², 陈国强^2,4

1 Department of Basic Medicine, Medical College of Qinghai University, Xining 810016, China;
2 MOE Key Lab of Bioinformatics and Systems Biology, Department of Biological Science and Biotechnology, School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China;
3 Tuspark Kunshan Biotech Science Park Development Center, 4/F, Science & Technology Plaza, Tsinghua Science Park, Kunshan, Jiangsu 215347, China;
4 Center for Nano and Micro Mechanics, Tsinghua University, Beijing 100084, China

通讯作者: CHEN Guoqiang
基金资助:
Supported by the National Natural Science Foundation of China (31260015), Natural Science Foundation of Qinghai Province (2012-Z-919Q), the Extramural Project from State Key Laboratory for Agrobiotechnology (2012SKLAB06-5) and the Research Funds for Young Project of Qinghai University (2011-QYY-1)

Abstract

Abstract: Synthetic biology promises to simplify the construction of metabolic pathways by assembling the detached modules of the whole pathway. This gives new approaches for the microbial production of industrial products such as polyhydroxyalkanoates (PHA). In this study, to produce poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) by Pseudomonas stutzeri 1317 from unrelated carbon sources such as glucose, the phaC1-phaZ-phaC2 operon of P. stutzeri 1317 was knocked out to generate the PHA deficient mutant P. stutzeri 1317LF. Then three modules containing phaC_AhA_ReB_Re, phaC_AhB_ReG_Pp and phaC_AhP_Ah were introduced into P. stutzeri 1317LF separately. The shake flask results indicated that the precursor supply and PHA synthase activity were the vital factors for the PHBHHx accumulation of P. stutzeri 1317LF. Furthermore, the PHBHHx accumulation of the recombinants from different carbon resources were performed. The highest PHBHHx content was 23.7%(by mass) with 58.6%(by mole) 3HB fraction. These results provide basis for further improving the PHBHHx accumulation of P. stutzeri from unrelated carbon sources.

Key words: Pseudomonas stutzeri 1317, PHBHHx, unrelated carbon sources

摘要： Synthetic biology promises to simplify the construction of metabolic pathways by assembling the detached modules of the whole pathway. This gives new approaches for the microbial production of industrial products such as polyhydroxyalkanoates (PHA). In this study, to produce poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) by Pseudomonas stutzeri 1317 from unrelated carbon sources such as glucose, the phaC1-phaZ-phaC2 operon of P. stutzeri 1317 was knocked out to generate the PHA deficient mutant P. stutzeri 1317LF. Then three modules containing phaC_AhA_ReB_Re, phaC_AhB_ReG_Pp and phaC_AhP_Ah were introduced into P. stutzeri 1317LF separately. The shake flask results indicated that the precursor supply and PHA synthase activity were the vital factors for the PHBHHx accumulation of P. stutzeri 1317LF. Furthermore, the PHBHHx accumulation of the recombinants from different carbon resources were performed. The highest PHBHHx content was 23.7%(by mass) with 58.6%(by mole) 3HB fraction. These results provide basis for further improving the PHBHHx accumulation of P. stutzeri from unrelated carbon sources.

关键词: Pseudomonas stutzeri 1317, PHBHHx, unrelated carbon sources

WEI Xiaoxing, LIU Feng, JIAN Jia, WANG Ruiyan, CHEN Guoqiang, . Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Recombinant Pseudomonas stutzeri 1317 from Unrelated Carbon Sources[J]. Chin.J.Chem.Eng., 2013, 21(9): 1057-1061.

魏晓星, 刘峰, 简嘉, 王瑞妍, 陈国强. Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Recombinant Pseudomonas stutzeri 1317 from Unrelated Carbon Sources[J]. Chinese Journal of Chemical Engineering, 2013, 21(9): 1057-1061.

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Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Recombinant Pseudomonas stutzeri 1317 from Unrelated Carbon Sources

Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Recombinant Pseudomonas stutzeri 1317 from Unrelated Carbon Sources

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