SCI和EI收录∣中国化工学会会刊

›› 2010, Vol. 18 ›› Issue (2): 312-321.

• BIOTECHNOLOGY AND BIOENGINEERING • Previous Articles     Next Articles

Antioxidant Activity of Isothiocyanate Extracts from Broccoli

YUAN Haina1,2, YAO Shanjing1, YOU Yuru2, XIAO Gongnian2, YOU Qi3   

  1. 1. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
    2. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China;
    3. Department of Food Science and Human Nutrition, Clemson University, Clemson SC, 29634, United States
  • Received:2009-11-02 Revised:2010-01-14 Online:2010-04-28 Published:2010-04-28
  • Supported by:
    Supported by the Natural Science Foundation of Zhejiang Province(Y3090135)

Antioxidant Activity of Isothiocyanate Extracts from Broccoli

袁海娜1,2, 姚善泾1, 尤玉如2, 肖功年2, 尤琦3   

  1. 1. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;
    2. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China;
    3. Department of Food Science and Human Nutrition, Clemson University, Clemson SC, 29634, United States
  • 通讯作者: YAO Shanjing,E-mail:yaosj@zju.edu.cn
  • 基金资助:
    Supported by the Natural Science Foundation of Zhejiang Province(Y3090135)

Abstract: Isothiocyanates(ITCs) extracts were prepared from fresh broccoli.Their antioxidant properties were evaluated by using the in vitro bioassays,including superoxide anion radical(O2-), hydroxyl radical(HO-) and 2,2-diphenyl-1-picrylhydracyl(DPPH·) radical-scavenging methods,lipid peroxidation assay,and reducing power assay.The ITCs extracts exhibited significant dose-dependent antioxidant activities(P<0.01) .Its antioxidant-stability was affected by temperature and storage condition,although it was more stable than vitamin C(Vc) .GC-MS iden-tified sulforaphane in the ITCs extracts.The antioxidant activity of sulforaphane was approximately one tenth to one fifth of that of Vc.Sulforaphane was experimented to be one of dominating contributors to antioxidant capacity of ITCs extracts.However,there was no direct antioxidant activity found for benzyl isothiocyanate(BITC) in vitro. Therefore,it could be concluded that the general group(-N=C=S) was not the essential part for the antioxidant activity of sulforaphane.It is suggested that the ITCs extracts from broccoli can be developed into a promising food additive for healthy food.

Key words: antioxidant activity, isothiocyanates, broccoli, sulforaphane

摘要: Isothiocyanates(ITCs) extracts were prepared from fresh broccoli.Their antioxidant properties were evaluated by using the in vitro bioassays,including superoxide anion radical(O2-), hydroxyl radical(HO-) and 2,2-diphenyl-1-picrylhydracyl(DPPH·) radical-scavenging methods,lipid peroxidation assay,and reducing power assay.The ITCs extracts exhibited significant dose-dependent antioxidant activities(P<0.01) .Its antioxidant-stability was affected by temperature and storage condition,although it was more stable than vitamin C(Vc) .GC-MS iden-tified sulforaphane in the ITCs extracts.The antioxidant activity of sulforaphane was approximately one tenth to one fifth of that of Vc.Sulforaphane was experimented to be one of dominating contributors to antioxidant capacity of ITCs extracts.However,there was no direct antioxidant activity found for benzyl isothiocyanate(BITC) in vitro. Therefore,it could be concluded that the general group(-N=C=S) was not the essential part for the antioxidant activity of sulforaphane.It is suggested that the ITCs extracts from broccoli can be developed into a promising food additive for healthy food.

关键词: antioxidant activity, isothiocyanates, broccoli, sulforaphane