Preparation of Supermacroporous Composite Cryogel Embedded with SiO2 Nanoparticles

Chin.J.Chem.Eng. ›› 2010, Vol. 18 ›› Issue (4): 667-671.

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Preparation of Supermacroporous Composite Cryogel Embedded with SiO₂ Nanoparticles

XU Panping¹, YAO Yuchen², SHEN Shaochuan¹, YUN Junxian¹, YAO Kejian¹

1. State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China;
2. School of Chemical Engineering, University of New South Wales, Sydney NSW 2052, Australia

Received:2010-01-13 Revised:2010-04-23 Online:2010-08-28 Published:2010-08-28

Preparation of Supermacroporous Composite Cryogel Embedded with SiO₂ Nanoparticles

许潘萍¹, 姚雨辰², 沈绍传¹, 贠军贤¹, 姚克俭¹

1. State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China;
2. School of Chemical Engineering, University of New South Wales, Sydney NSW 2052, Australia

通讯作者: YAO Kejian, E-mail:yaokj@zjut.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(20876145);the Natural Science Foundation of Zhejiang Province(Y4080329)

Abstract

Abstract: Supermacroporous composite cryogels embedded with SiO₂ nanoparticles were prepared by radical cryogenic copolymerization of the reactive monomer mixture of acrylamide (AAm) and N,N-methylene-bis-acrylamide (MBAAm) containing SiO₂ nanoparticles (mass ratios of nanoparticles to the monomer AAm from 0.01 to 0.08) under the freezing-temperature variation condition in glass columns.The properties of these composite cryogels were measured.The height equivalent to theoretical plate(HETP) of the cryogel beds at different liquid flow rates was determined by residence time distribution (RTD) using tracer pulse-response method.The composite cryogel matrix embedded with the mass fraction of SiO₂ nanoparticles of 0.02 presented the best properties and was employed in the following graft polymerization.Chromatographic process of lysozyme in the composite cryogel grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPSA) was carried out to evaluate the protein breakthrough and elution characteristics.The chromatography can be carried out at relatively high superficial velocity,i.e.,15 cm·min^-1,indicating the satisfactory mechanical strength due to the embedded nanoparticles.

Key words: composite cryogel, SiO₂ nanoparticle, protein chromatography

摘要： Supermacroporous composite cryogels embedded with SiO₂ nanoparticles were prepared by radical cryogenic copolymerization of the reactive monomer mixture of acrylamide (AAm) and N,N-methylene-bis-acrylamide (MBAAm) containing SiO₂ nanoparticles (mass ratios of nanoparticles to the monomer AAm from 0.01 to 0.08) under the freezing-temperature variation condition in glass columns.The properties of these composite cryogels were measured.The height equivalent to theoretical plate(HETP) of the cryogel beds at different liquid flow rates was determined by residence time distribution (RTD) using tracer pulse-response method.The composite cryogel matrix embedded with the mass fraction of SiO₂ nanoparticles of 0.02 presented the best properties and was employed in the following graft polymerization.Chromatographic process of lysozyme in the composite cryogel grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPSA) was carried out to evaluate the protein breakthrough and elution characteristics.The chromatography can be carried out at relatively high superficial velocity,i.e.,15 cm·min^-1,indicating the satisfactory mechanical strength due to the embedded nanoparticles.

关键词: composite cryogel, SiO₂ nanoparticle, protein chromatography

XU Panping, YAO Yuchen, SHEN Shaochuan, YUN Junxian, YAO Kejian. Preparation of Supermacroporous Composite Cryogel Embedded with SiO₂ Nanoparticles[J]. Chin.J.Chem.Eng., 2010, 18(4): 667-671.

许潘萍, 姚雨辰, 沈绍传, 贠军贤, 姚克俭. Preparation of Supermacroporous Composite Cryogel Embedded with SiO₂ Nanoparticles[J]. Chinese Journal of Chemical Engineering, 2010, 18(4): 667-671.

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Preparation of Supermacroporous Composite Cryogel Embedded with SiO₂ Nanoparticles

Preparation of Supermacroporous Composite Cryogel Embedded with SiO₂ Nanoparticles

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