Space-flight mutation of streptomyces gilvosporeus for enhancing natamycin production

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Space-flight mutation of streptomyces gilvosporeus for enhancing natamycin production

LIANG Jingle^1,2, LIN Jianping¹; XU Zhinan¹; SU Wei²; CEN Peilin¹

¹ Institute of Bioengineering, College of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China
² Shandong Lukang Pharmaceutical Co., Jining 272021, China

Received:2007-01-09 Revised:1900-01-01 Online:2007-10-28 Published:2007-10-28
Contact: LIANG Jingle

航天诱变提高Streptomyces gilvosporeus的那他霉素产量

梁景乐^1,2; 林建平¹; 徐志南¹; 苏薇²; 岑沛霖¹

¹ Institute of Bioengineering, College of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China
² Shandong Lukang Pharmaceutical Co., Jining 272021, China

通讯作者: 梁景乐

Abstract

Abstract: Mutants of the strain producing natamycin, Streptomyces gilvosporeus, were obtained after space-flight mutation. With respect to the sand spores and slant spores, the mutation ratios were up to 67.6% and 78.3% and the survival ratio was 43.1% and 3.0%, respectively. An improved mutant producing natamycin, S. gilvosporeus LK-45, was screened, which showed natamycin productivity of 1420mg•L－1. A mutant resistant to 2-deoxy glucose, S. gil-vosporeus LK-119, was further obtained using a rational screening procedure. The natamycin productivity of 1940mg•L－1 was achieved when glucose was used as the carbon source.

Key words: space-flight mutation, Streptomyces gilvosporeus, natamycin, rational screening, 2-deoxy glucose

摘要： Mutants of the strain producing natamycin, Streptomyces gilvosporeus, were obtained after space-flight mutation. With respect to the sand spores and slant spores, the mutation ratios were up to 67.6% and 78.3% and the survival ratio was 43.1% and 3.0%, respectively. An improved mutant producing natamycin, S. gilvosporeus LK-45, was screened, which showed natamycin productivity of 1420mg•L－1. A mutant resistant to 2-deoxy glucose, S. gil-vosporeus LK-119, was further obtained using a rational screening procedure. The natamycin productivity of 1940mg•L－1 was achieved when glucose was used as the carbon source.

关键词: space-flight mutation;Streptomyces gilvosporeus;natamycin;rational screening;2-deoxy glucose

LIANG Jingle, LIN Jianping, XU Zhinan, SU Wei, CEN Peilin. Space-flight mutation of streptomyces gilvosporeus for enhancing natamycin production[J]. , DOI: .

梁景乐, 林建平, 徐志南, 苏薇, 岑沛霖. 航天诱变提高Streptomyces gilvosporeus的那他霉素产量[J]. , DOI: .

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