Transport of nanoparticles and coagulation is simulated with the combination of CFD in a circular bend. The Taylor-expansion moment method(TEMOM)is employed to study dynamics of nanoparticles with Brownian motion,based on the flow field from numerical simulation.A fully developed flow pattern in the present simulation is compared with previous numerical results for validating the model and computational code.It is found that for the simulated particulate flow system,the particle mass concentration,number concentration,particle polydispersity, mean particle diameter and geometric standard deviation over cross-section increase with time.The distribution of particle mass concentration at different time is independent of the initial particle size.More particles are concentrated at outer edge of the bend.Coagulation plays more important role at initial stage than that in the subsequent period.The increase of Reynolds number and initial particle size leads to the increase of particle number concentration.The particle polydispersity,mean particle diameter and geometric standard deviation increase with decreasing Reynolds number and initial particle size.

This paper reports an investigation of Computational Fluid Dynamics(CFD) on the influence of injection momentum rate of premixed air and fuel on the flameless Moderate or Intense Low oxygen Dilution(MILD) combustion in a recuperative furnace.Details of the furnace flow velocity,temperature,O₂,CO₂ and NO_x concentrations are provided.Results obtained suggest that the flue gas recirculation plays a vital role in establishing the premixed MILD combustion.It is also revealed that there is a critical momentum rate of the fuel-air mixture below which MILD combustion does not occur.Moreover,the momentum rate appears to have less significant influence on conventional global combustion than on MILD combustion.

The chaotic characteristics of bubbles rising with accompanying coalescences in pseudoplastic aqueous carboxymethylcellulose sodium(CMC)solution were studied by means of smoothed pseudo Wigner-Ville distribution and Wigner-Hough distribution.The temporal signal of bubble passage was measured utilizing a photoconductive data acquisition system.As bubble coalescence occurred,the smoothed pseudo Wigner-Ville distribution of the signal revealed that the signal could be divided into low-frequency and high-frequency ranges and the transition range according to the distribution feature of frequency domain,which reflected eddy motion of fluid,high frequency fluctuations of fluid velocity and other random components measured in the signal,and bubbles rising accompanied with coalescences,respectively.However,bubble coalescence occurred in the lower position and the frequency range of bubbles motion became wide under higher gas flowrate,while the frequency range of bubbles motion became narrow when the CMC concentration increased.The typical dynamics of bubbles motion,such as periodicity,bifurcation and chaos,could be easily found in terms of the Wigner-Hough distribution.

Based on trajectory equations of gas bubble,an eddy-bubble interaction(EBI)model was developed. This model considered the effect of non-drag forces and took the eddy-bubble interaction time as the refreshing time scale of turbulent fluctuations.The relationship between the crossingeddy time and the eddy lifetime was discussed,and the predicted distributions of radial,axial velocities of bubbles and gas holdup were also given. Compared with eddy lifetime(EL)model,the EBI model gives somewhat smaller axial velocity in the upper circulation region and larger velocity in the lower circulation region,causing that fewer bubbles reach the lower circulation region and gas holdup becomes higher in the upper circulation region.The predicted gas holdup by the EBI model approaches closer to the experimental data in the discharge stream region.

As a high gravity(HIGEE) unit,the rotating packed bed(RPB) uses centrifugal force to intensify mass transfer.Zigzag rotating bed(RZB) is a new type of HIGEE unit.The rotor of RZB consists of stationary discs and rotating discs,forming zigzag channels for liquid-gas flow and mass transfer.As in RPBs,some hydrodynamic behavior in RZB is interesting but no satisfactory explanation.In this study,the experiments were carried on in a RZB unit with a rotor of 600 mm in diameter using air-water system.The gas pressure drop and power consumption were measured with two types of rotating baffle for RZB rotors,one with perforations and another with shutter openings. The circumferential velocities of gas were measured with a five-hole Pitot probe.The pressure drop decreased rapidly when the liquid was introduced to the rotor,because the circumferential velocity of the liquid droplets was lower than that of the gas,reducing the circumferential velocity of gas and the centrifugal pressure drop.The power consumption decreased first when the gas entered the RZB rotor,because the gas with higher circumferential velocity facilitates the rotation of baffles.

A new one-dimensional system for resistivity measurement for natural gas hydrate(NGH) exploitation is designed,which is used to study the formation and decomposition processes of NGH.The experimental results verify the feasibility of the measurement method,especially in monitoring the nucleation and growth of the NGH. Isovolumetric formation experiment of NGH is performed at 2℃ and 7.8 MPa.Before the NGH formation,the initial resistivity is measured to be 4-7Ω·m,which declines to the minimum value of 2-3Ω·m when NGH begins to nucleate after the pressure is reduced to 3.3 MPa.As the NGH grows,the resistivity increases to a great extent,and finally it keeps at 11-13Ω·m,indicating the completion of the formation process.The NGH decomposition experiment is then performed.When the outlet pressure decreases,NGH begins to decompose,accordingly,the resistivity declines gradually,and is at 5-9Ω·m when the decomposition process ends,which is slightly higher than the resistivity value before the formation of NGH.The occurrence and distribution uniformity of NGH are determined by the distribution and magnitude of the resistivity measured on an one-dimensional sand-packed model.This study tackles the accurate estimation for the distribution of NGH in porous medium,and provides an experimental basis for further study on NGH exploitation in the future.

Batch extractive distillation(BED)is a special method used in the distillation process by adding a solvent into the batch distillation column to alter the relative volatility of the components and improve the separation. A comprehensive design and simulation method is required due to the complexity of BED.In this study,a quasi-steady-state model for BED is proposed,the derivation and solution of the model are presented.This shortcut model can be used to simulate the composition and temperature of the reboiler,the top and other plates of the column in a batch extractive distillation operation.The calculated values are in good agreement with the experimental data.The results show that the quasi-steady-state model is a practical method because of some advantages such as high precision and fast calculation.

The transport of Cu(Ⅱ)from aqueous solutions containing buffer media through hollow fiber supported liquid membrane(HFSLM)using di(2-ethylhexyl)phosphoric acid(D2EHPA)dissolved in kerosene as membrane phase and hydrochloric acid as striping phase was investigated.A set of factors were studied,including tube side velocity,shell side velocity,pH of the feed phase,Cu(Ⅱ)concentration in the feed phase,buffer media concentration and D2EHPA concentration in the membrane phase.Experimental results indicate that the mass transfer coefficient increases with increasing both carrier concentration in the organic phase and flow rates on the tube side and shell side,and decreases with increasing initial Cu(Ⅱ)concentration in the feed phase.With increasing pH value and acetate concentration in the feed phase,the mass transfer coefficient reaches a maximum value then decreases.The optimal operating conditions are obtained at pH value of 4.44 and 0.1 mol·L^-1 acetic ion oncentration in feed phase,and carrier volume fraction of around 10%in kerosene as organic phase.A mathematical model of the transport mechanism through HFSLM is developed.The modeled results agree well with the experimental ones.

For an alcohol/water system and with fin baffle packing,continuous distillation experiments were carried out in a rotating packed bed(RPB)system at atmospheric pressure.The effects of the average high gravity factor(β),liquid reflux ratio (R) and feedstock flux (F) on the momentum transfer and mass transfer were investigated.The gas phase pressure drop of RPB increased with the average high gravity factor,liquid reflux ratio and feedstock flux,which was 13.55-64.37 Pa at β of 2.01-51.49,R of 1.0-2.5,and F of 8-24 L·h^-1 for a theoretical tray in the RPB with fin baffle packing.The investigation on the mass transfer in the RPB with different packings showed that the number of transfer units of RPB with a packing also increased with the average high gravity factor,reflux ratio and feedstock flux.It is found that the fin baffle packing(packing Ⅲ)presents the best mass transfer performance and lowest pressure drop for the height equivalent to a theoretical plate(HETP),which is 6.59-9.84 mm.

The decomposition kinetics for formation of CO₂ hydrates in 90 cm³ wet natural silica sands were studied systematically using the depressurization method at the temperatures ranging from 273.2 to 277.2 K and the pressures from 0.5 to 1.0 MPa.The effects of temperature,pressure,particle diameter,porosity,and salinity of formation water on the decomposition kinetics were investigated.The results show that the dissociation percentage increases as temperature increases or as the initial decomposition pressure decreases.An increase in porosity or a decrease in particle diameter of silica sands accelerates the decomposition.Increasing the salinity of the formation water gives rise to a faster decomposition.However,a combination of the present results with the observations in literature reveals that the effect of the coexisting ionic solute depends on its chemical structure.

For high-purity distillation processes,it is difficult to achieve a good direct product quality control using traditional proportional-integral-differential(PID)control or multivariable predictive control technique due to some difficulties,such as long response time,many un-measurable disturbances,and the reliability and precision issues of product quality soft-sensors.In this paper,based on the first principle analysis and dynamic simulation of a distillation process,a new predictive control scheme is proposed by using the split ratio of distillate flow rate to that of bottoms as an essential controlled variable.Correspondingly,a new strategy with integrated control and on-line optimization is developed,which consists of model predictive control of the split ratio,surrogate model based on radial basis function neural network for optimization,and modified differential evolution optimization algorithm. With the strategy,the process achieves its steady state quickly,so more profit can be obtained.The proposed strategy has been successfully applied to a gas separation plant for more than three years,which shows that the strategy is feasible and effective.

A comparison of arithmetic operations of two dynamic process optimization approaches called quasi-sequential approach and reduced Sequential Quadratic Programming(rSQP)simultaneous approach with respect to equality constrained optimization problems is presented.Through the detail comparison of arithmetic operations,it is concluded that the average iteration number within differential algebraic equations(DAEs)integration of quasi-sequential approach could be regarded as a criterion.One formula is given to calculate the threshold value of average iteration number.If the average iteration number is less than the threshold value,quasi-sequential approach takes advantage of rSQP simultaneous approach which is more suitable contrarily.Two optimal control problems are given to demonstrate the usage of threshold value.For optimal control problems whose objective is to stay near desired operating point,the iteration number is usually small.Therefore,quasi-sequential approach seems more suitable for such problems.

This paper is concerned with the control performance assessment based on the multivariable generalized minimum variance benchmark.An explicit expression for the feedback controller-invariant(the generalized minimum variance)term of the multivariable control system is obtained,which is used as a standard benchmark for the assessment of the control performance for multi input multi output(MIMO)process.The proposed approach is based on the multivariable minimum variance benchmark.In comparison with the minimum variance benchmark, the developed method is more reasonable and practical for the control performance assessment of multivariable systems.The approach is illustrated by a simulation example and an industrial application.

An optimal control strategy is proposed to improve the fermentation titer,which combines the support vector machine(SVM)with real code genetic algorithm(RGA).A prediction model is established with SVM for penicillin fermentation processes,and it is used in RGA for fitting function.A control pattern is proposed to overcome the coupling problem of fermentation parameters,which describes the overall production condition.Experimental results show that the optimal control strategy improves the penicillin titer of the fermentation process by 22.88%,compared with the routine operation.

In this study,a new method is presented to correlate the shear viscosity of nanofluids by local composition theory.The Eyring theory and nonrandom two-liquid(NRTL)equation are used for this purpose.The effects of temperature and particle volume concentration on the viscosity are investigated.The adjustable parameters of NRTL equation are obtained by fitting with experimental data.The calculated shear viscosities for nanofluids of CuO/water with 29 nm particle size,Al₂O₃/water with two different particle diameters,36 nm and 47 nm,and CuO/(ethylene glycol,water)are compared with experimental data and the average absolute deviation(AAD)is 1.2%,while the results from some conventional models yield an AAD of 190%.The results of this study are in excellent agreement with experimental data.

A promising bacterial strain for the biodegradation of Microcystins(MCs)was isolated from Dianchi lake in China and identified as Sphingopyxis sp.USTB-05 by the analysis of 16s rDNA.Initial MC-RR of 42.3mg·L^-1 was completely degraded by USTB-05 within 36 h,which was a relatively high biodegradation rate of MC-RR.With the cell-free extract(CE)of Sphingopyxis sp.USTB-05,MC-RR was biodegraded at a more rapid biodegradation rate compared with its strain,so that initial MC-RR of 42.3mg·L^-1 was completely biodegraded within 10h.During the bio-reaction of MC-RR catalyzed by CE,two intermediate metabolites and a dead-end product of MC-RR were observed on HPLC profiles and all of them had similar scanning profiles in the wavelength from 200 to 300 nm,indicating that the group of Adda in all products of MC-RR remained intact.

A novel bioactive foam emulsion bioreactor for benzene,toluene and xylene(BTX)contaminated air streams treatment has been developed.The gas-liquid interfacial area by biocompatible foam and driving force for mass transfer by a water immiscible organic phase were increased in this reactor.The effect of several parameters such as gas residence time,oxygen content,and organic phase concentration on bioreactor performance was studied. Experimental results showed an average elimination capacity(EC)of 220 g·m^-3·h^-1 with removal efficiency(RE) of 89.59%for BTX inlet concentration of 1g·m^-3 at 15s gas residence time in the bioreactor.The statistical developed model predicted that the maximum elimination capacity of the reactor for BTX could be reached to 423.45g·m^-3·h^-1.Continues operation of the bioreactor with high EC and RE was demonstrated by optimizing the operational parameters of the bioreactor.Overall the results suggest that the bioreactor developed can be very effective systems to treat BTX vapors.

A neutral metalloprotease was purified from the cultured mycelia of Laccocephalum mylittae,an effective medicinal fungus widely used in anthelmintic therapy.The protease was purified to homogeneity with 31.85-fold purification and a final yield of 21.76%.The subunit molecular weight of the protease is about 40000 estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE).The optimum reaction pH and temperature are 7.5 and 50℃,respectively.The protease activity is largely enhanced by Ca²⁺,but highly inhibited by tetrasodium ethylenediaminetetraacetate(EDTA),a metal-chelator,suggesting that the enzyme is a metalloprotease.The Michaelis-Menten constan Km and Vmax value for casein substrate are 6.09mg·ml^-1and 21.32μg·min^-1·ml^-1,respectively.In vitro anthelmintic tests of the protease exhibit distinct lethal effects on the third stage larvae(L3)of Ascaris suum.Scanning electron microscopy and SDS-PAGE analysis indicates that the proteolysis of larvae proteins caused by this protease may relate to the anthelmintic activity of L.mylittae.

Fragment containing the whole riboflavin(rib)operons of B.cereus ATCC14579 was detected from GenBank and annotated.The rib operon of ATCC14579 was cloned with Pn,its native promoter,or with P43,the vegetative growth promoter,into the plasmid.Expression analysis showed that heterologous rib operon was operative in B.subtilis.Integrative plasmid with P43-rib fragment was integrated into the chromosome of B.subtilis RH33,yielding transformant B.subtilis PY.With optimized medium components,4.3 g·L^-1 of riboflavin was achieved in batch culture of B.subtilis PY,which was 27%enhancement compared to the host strain.Real-time reverse transcription polymerase chain reaction(RT-PCR)analysis indicated that the transcriptional level of ribA maintained 2.8-fold higher with the expression of herterologous riboperon.Furthermore,the stability of B.subtilis PY was increased form 45%to 87%.The high transcriptional level of rib gene and higher stability of B.subtilis PY could explain the increased riboflavin production.

In order to improve the production of human-like collagen Ⅲ(HLC Ⅲ)by fed-batch culture of recombinant Escherichia coli BL21,the Plackett-Burman and Box-Behnken design were applied to optimize the fermentation process parameters.Three variables(induction time,inoculum age and pH),which have significant effects on HLC Ⅲ production,were selected from eight variables by Plackett-Burman design.With the regression coefficient analysis in the Box-Behnken design,a relationship between HLC Ⅲ production and three significant factors was obtained,and the optimum levels of the three variables were as follows:induction time 3.2h,inoculum age 12.6 h and pH 6.7.The 3D response surface plots and 2D contour plots created by the Box-Behnken design showed that the interaction between induction time and pH and that between innoculum age and pH were significant.An average 9.68 g·L^-1HLC Ⅲ production was attained in the validation experiment under optimized condition,which was 80%higher than the yield of 5.36 g·L^-1before optimization.

An experimental study of thermal DeNO_x process with different additives was performed in an electricityheated tubular flow reactor,showing that CO is less effective to lower the optimum temperature than H₂ and CH₄.The maximum NO reduction is lowered with H₂ added,while it is hardly affected by CO or CH₄.The temperature window is widened appreciably with CH₄ added,while it is narrowed slightly by H₂ or CO.The disadvantage of CH₄ is that it causes CO emission due to its incomplete oxidation,and the maximum conversion of CH₄ to CO is more than 50%.In general,the calculation using a detailed chemical kinetic model predicts most of the process features reasonably well.The analysis on reaction mechanism shows that the effects of these additives on NO reduction are achieved principally by promoting the production of·OH radical.

The microcapsules with cores of ethylenediamine tetraacetic acid tetrasodium salt (Na₄-EDTA) and walls of polyurea were synthesized via an interfacial polycondensation reaction witH₂,4-tolylene diisocyanate as an oil-soluble monomer and diethyl triamine as a water-soluble monomer.Various manufacturing parameters,including the amount of emulsifier,agitation speed,stirring time and ratios of the wall materials to core materials,were altered to optimize process variables during the synthesis of microcapsules,and the effects of these parameters on the characteristics of the microcapsules were examined.The structure,morphology,mean particle size and size distribution were characterized by optical microscope and scanning electron microscopy(SEM),showing that the mean diameter of optimal microspheres was approximately 6μm,and microcapsules were spherical.In vitro release of Na₄-EDTA from these microcapsules was performed in distilled water.Under the optimal preparation conditions, the Na₄-EDTA release profiles were biphasic with a burst release followed by a gradual release phase.After an initial burst,a continuous Na₄-EDTA release was up to 5-7 days.The optimal synthesis conditions for the microcapsules with stable,good morphology and good controlled-release properties were as follows:emulsifier Span-80 10% (by mass),agitation speed 900r·min1,stirring time 30 min,and the ratio of the wall materials to core materials 0.15.

A novel polymer/SiO₂ hybrid emulsion(PAES) was prepared by directly mixing colloidal silica with polyacrylate emulsion(PAE)modified by a saline coupling agent.The sol-gel-derived thin films were obtained by addition of co-solvents into the PAES.The effects of γ-methacryloxypropy ltrimethoxysilane(KH-570) content and co-solvent on the properties of PAES films were investigated.Dynamic laser scattering(DLS) data indicate that the average diameter of PAES(96 nm) is slightly larger than that of PAE(89 nm).Transmission electron microscopy (TEM) photo discloses that colloidal silica particles are dispersed uniformly around polyacrylate particles and some of the colloidal silica particles are adsorbed on the surface of PAE particles.The crosslinking degree data and Fourier transform infrared(FT-IR)spectra confirm that the chemical structure of the PAES is changed to form Si-O-Si-polymer crosslinking networks during the film formation.Atomic force microscope(AFM) photos show the solvent induced sol-gel process of colloidal silica and the Si-based polymer distribution on the film surface of the dried PAES.Thermogravimetric analysis(TGA) curves demonstrate that the PAES films display much better thermal stability than PAE.

In order to analyze the influence of vapor cloud shape on temperature field effect of unconfined vapor cloud explosion(UVCE) and obtain creditable prediction method of explosion temperature effect,the transient temperature fields of cylindrical and hemispherical UVCEs with same methane concentration and mass were numerically studied by computational fluid dynamics(CFD) technology.According to numerical simulation results, the concepts of UVCE's temperature-near-field and temperature-far-field were proposed,the corresponding ranges were given,and the temperature attenuation laws and differences in corresponding regions with different vapor cloud shapes were presented.Through comparing with Baker fireball model,the accuracy and visualizability in acquisition of entire temperature effect based on numerical simulation were further validated.The functional relations among maximum temperature,horizontal distance,initial temperature and vapor cloud mass in temperature-near-field and temperature-far-field were deduced by means of data fitting,respectively.These conclusions provided quantitative basis for forecast and protection of UVCE disaster.

Enhanced oil recovery(EOR) by means of polymer flooding is an important technology for the strategic development of offshore oilfields in China.Hydrophobically associating polyacrylamide(HAPAM) has been recently proposed as a new flooding agent.The solubility of HAPAM is low,which is the bottleneck for further improving the oil recovery through polymer flooding in offshore oilfield.Stirred tanks have been used on offshore platforms to enhance HAPAM dissolving.But there is little literature on the study of HAPAM dissolving characteristics in stirred tanks.In this paper,effects of temperature,salinity,stirring speed,impeller type and stirring method on the dissolution of HAPAM are reported.The experimental results manifest that the dissolving rate of HAPAM increases with temperature and stirring speed,but the viscosity of the polymer solution decreases.There is an optimal range of salinity for polymer dissolving.Combining the operation mode of up-pumping with varying stirring speed,hydrofoil impeller can accelerate the dissolution of HAPAM and maintain a high solution viscosity.