Characterization of Lignins Isolated from Alkali Treated Prehydrolysate of Corn Stover

doi:10.1016/S1004-9541(13)60468-1

Chin.J.Chem.Eng. ›› 2013, Vol. 21 ›› Issue (4): 427-433.DOI: 10.1016/S1004-9541(13)60468-1

• BIOTECHNOLOGY AND BIOENGINEERING • Previous Articles Next Articles

Characterization of Lignins Isolated from Alkali Treated Prehydrolysate of Corn Stover

LEI Mingliu^1,2, ZHANG Hongman^1,2, ZHENG Hongbo¹, LI Yuanyuan^1,2, HUANG He², XU Rong ^1,2

1 College of Science, Nanjing University of Technology, Nanjing 210009, China;
2 China State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China

Received:2011-12-26 Revised:2012-08-06 Online:2013-04-26 Published:2013-04-28
Supported by:
Supported by the National Natural Science Foundation of China (20876078, 21176124), the National High Technology Research and Development Program of China (2011AA02A207), the National Basic Research Program of China (2009CB724700), the Key Program of the National Natural Science Foundation of China (20936002), and the Independent Innovation Project of Jiangsu Province (CX(11)2051).

Characterization of Lignins Isolated from Alkali Treated Prehydrolysate of Corn Stover

雷鸣柳^1,2, 张红漫^1,2, 郑洪波¹, 李媛媛^1,2, 黄和², 徐蓉^1,2

1 College of Science, Nanjing University of Technology, Nanjing 210009, China;
2 China State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China

通讯作者: ZHANG Hongman
基金资助:
Supported by the National Natural Science Foundation of China (20876078, 21176124), the National High Technology Research and Development Program of China (2011AA02A207), the National Basic Research Program of China (2009CB724700), the Key Program of the National Natural Science Foundation of China (20936002), and the Independent Innovation Project of Jiangsu Province (CX(11)2051).

Abstract

Abstract: Lignins were isolated and purified from alkali treated prehydrolysate of corn stover. The paper presents the structural features of lignins in a series purification processes. Fourier transform infrared spectroscopy, ultraviolet-vis spectroscopy and proton nuclear magnetic resonance spectroscopy were used to analyze the chemical structure. Thermogravimetric analysis was applied to follow the thermal degradation, and wet chemical method was used to determine the sugar content. The results showed that the crude lignin from the prehydrolysate of corn stover was a heterogeneous material of syringyl, guaiacyl and p-hydroxyphenyl units, containing associated polysaccharides, lipids, and melted salts. Some of the crude lignin was chemically linked to hemicelluloses (mainly xylan). The lipids in crude lignin were probably composed of saturated and/or unsaturated long carbon chains, fatty acids, triterpenols, waxes, and derivatives of aromatic. The sugar content of purified lignin was less than 2.11%, mainly composed of guaiacyl units. DTG_max of purified lignin was 359℃. The majority of the hydroxyl groups were phenolic hydroxyl groups. The main type of linkages in purified lignin was β-O-4. Other types of linkages included β-5, β-β and α-O-4.

Key words: lignin structure, cellulose ethanol, alkaline pretreatment, corn stover, purification

摘要： Lignins were isolated and purified from alkali treated prehydrolysate of corn stover. The paper presents the structural features of lignins in a series purification processes. Fourier transform infrared spectroscopy, ultraviolet-vis spectroscopy and proton nuclear magnetic resonance spectroscopy were used to analyze the chemical structure. Thermogravimetric analysis was applied to follow the thermal degradation, and wet chemical method was used to determine the sugar content. The results showed that the crude lignin from the prehydrolysate of corn stover was a heterogeneous material of syringyl, guaiacyl and p-hydroxyphenyl units, containing associated polysaccharides, lipids, and melted salts. Some of the crude lignin was chemically linked to hemicelluloses (mainly xylan). The lipids in crude lignin were probably composed of saturated and/or unsaturated long carbon chains, fatty acids, triterpenols, waxes, and derivatives of aromatic. The sugar content of purified lignin was less than 2.11%, mainly composed of guaiacyl units. DTG_max of purified lignin was 359℃. The majority of the hydroxyl groups were phenolic hydroxyl groups. The main type of linkages in purified lignin was β-O-4. Other types of linkages included β-5, β-β and α-O-4.

关键词: lignin structure, cellulose ethanol, alkaline pretreatment, corn stover, purification

LEI Mingliu, ZHANG Hongman, ZHENG Hongbo, LI Yuanyuan, HUANG He, XU Rong . Characterization of Lignins Isolated from Alkali Treated Prehydrolysate of Corn Stover[J]. Chin.J.Chem.Eng., 2013, 21(4): 427-433.

雷鸣柳, 张红漫, 郑洪波, 李媛媛, 黄和, 徐蓉. Characterization of Lignins Isolated from Alkali Treated Prehydrolysate of Corn Stover[J]. Chinese Journal of Chemical Engineering, 2013, 21(4): 427-433.

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Characterization of Lignins Isolated from Alkali Treated Prehydrolysate of Corn Stover

Characterization of Lignins Isolated from Alkali Treated Prehydrolysate of Corn Stover

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