Production of paeoniflorin and albiflorin by callus tissue culture of Paeonia lactiflora Pall

doi:10.1016/j.cjche.2014.06.036

›› 2015, Vol. 23 ›› Issue (2): 451-455.DOI: 10.1016/j.cjche.2014.06.036

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Production of paeoniflorin and albiflorin by callus tissue culture of Paeonia lactiflora Pall

Sheng Hu¹, Yingying Ma², Hailong Jiang³, Dingjun Feng³, Wei Yu³, Dongmei Dai¹, Lehe Mei^1,2

1 College of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China;
2 Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China;
3 Ningbo Liwah Pharmaceutical Co., Ltd., Ningbo 315174, China

Received:2013-10-03 Revised:2014-06-16 Online:2015-03-01 Published:2015-02-28
Supported by:
Supported by the National Natural Science Foundation of China (21176220, 31240054), Key Technology Research and Development Project of Ningbo (2011C11023), and Zhejiang Provincial Natural Science Foundation of China (Z13B060008).

Production of paeoniflorin and albiflorin by callus tissue culture of Paeonia lactiflora Pall

Sheng Hu¹, Yingying Ma², Hailong Jiang³, Dingjun Feng³, Wei Yu³, Dongmei Dai¹, Lehe Mei^1,2

1 College of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China;
2 Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China;
3 Ningbo Liwah Pharmaceutical Co., Ltd., Ningbo 315174, China

通讯作者: Lehe Mei
基金资助:
Supported by the National Natural Science Foundation of China (21176220, 31240054), Key Technology Research and Development Project of Ningbo (2011C11023), and Zhejiang Provincial Natural Science Foundation of China (Z13B060008).

Abstract

Abstract: In order to facilitate the preparation of paeoniflorin (PF) and albiflorin (AF), two chief bioactive constituents in Paeonia lactiflora Pall (PL), induction and culture of callus from PL were studied. With a modified woody plant medium supplemented with 0.5 mg·L^-1 6-benzylaminopurine, 1.0 mg·L^-1 naphthylacetic acid, 0.1 mg·L^-1 thidiazuron and 30 g·L^-1 sucrose, callus was induced from four kinds of explants: leaf, stems, petiole, and root. The potency to formcallus varies between different explants and leaf explants exhibits the highest capacity (100%). On the other hand, root-derived callus (R-callus) produces the highest level of total amount of PF and AF, 31.8 mg·g^-1 dry mass, which is higher than the corresponding level in the root of field cultivated PL. Furthermore, the timeneeded is only 40 days, remarkably shorter than the cultivation timeof PL, about 4-5years. Higher accumulation levels of PF and AF with shorter production time indicate that callus culture of PL is a promising powerful tool for production of PF and AF in the future.

Key words: Albiflorin, Paeoniflorin, Callus, Plant cell cultures, Paeonia lactiflora Pall

摘要： In order to facilitate the preparation of paeoniflorin (PF) and albiflorin (AF), two chief bioactive constituents in Paeonia lactiflora Pall (PL), induction and culture of callus from PL were studied. With a modified woody plant medium supplemented with 0.5 mg·L^-1 6-benzylaminopurine, 1.0 mg·L^-1 naphthylacetic acid, 0.1 mg·L^-1 thidiazuron and 30 g·L^-1 sucrose, callus was induced from four kinds of explants: leaf, stems, petiole, and root. The potency to formcallus varies between different explants and leaf explants exhibits the highest capacity (100%). On the other hand, root-derived callus (R-callus) produces the highest level of total amount of PF and AF, 31.8 mg·g^-1 dry mass, which is higher than the corresponding level in the root of field cultivated PL. Furthermore, the timeneeded is only 40 days, remarkably shorter than the cultivation timeof PL, about 4-5years. Higher accumulation levels of PF and AF with shorter production time indicate that callus culture of PL is a promising powerful tool for production of PF and AF in the future.

关键词: Albiflorin, Paeoniflorin, Callus, Plant cell cultures, Paeonia lactiflora Pall

Sheng Hu, Yingying Ma, Hailong Jiang, Dingjun Feng, Wei Yu, Dongmei Dai, Lehe Mei. Production of paeoniflorin and albiflorin by callus tissue culture of Paeonia lactiflora Pall[J]. , 2015, 23(2): 451-455.

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Production of paeoniflorin and albiflorin by callus tissue culture of Paeonia lactiflora Pall

Production of paeoniflorin and albiflorin by callus tissue culture of Paeonia lactiflora Pall

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