Characterization of wet microalgal cells pretreated with steam for lipid extraction

doi:10.1016/j.cjche.2020.09.028

Chinese Journal of Chemical Engineering ›› 2021, Vol. 37 ›› Issue (9): 114-120.DOI: 10.1016/j.cjche.2020.09.028

• Biotechnology and Bioengineering • Previous Articles Next Articles

Characterization of wet microalgal cells pretreated with steam for lipid extraction

Wenlu Song¹, Rui Huang², Hao Guo³, Chunguang Yin¹, Chuanling Wang⁴, Jun Cheng³, Weijuan Yang³

1. Department of Life Science and Engineering, Jining University, Jining 273155, China;
2. The Electrical Engineering College, Guizhou University, Guiyang 550025, China;
3. State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China;
4. ShanDong Fanpu Analytic Co., Ltd, Jining 272000, China

Received:2020-06-19 Revised:2020-09-14 Online:2021-11-02 Published:2021-09-28
Contact: Jun Cheng
Supported by:
This study was supported by the National Key Research and Development Program-China (2017YFE0122800), Shandong Provincial Natural Science Foundation (ZR2019MC060), Key Research and Development Program of Jining City (2018ZDGH024), and a Project of Shandong Province Higher Educational Science and Technology Program (J17KA095).

Characterization of wet microalgal cells pretreated with steam for lipid extraction

Wenlu Song¹, Rui Huang², Hao Guo³, Chunguang Yin¹, Chuanling Wang⁴, Jun Cheng³, Weijuan Yang³

1. Department of Life Science and Engineering, Jining University, Jining 273155, China;
2. The Electrical Engineering College, Guizhou University, Guiyang 550025, China;
3. State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China;
4. ShanDong Fanpu Analytic Co., Ltd, Jining 272000, China

通讯作者: Jun Cheng
基金资助:
This study was supported by the National Key Research and Development Program-China (2017YFE0122800), Shandong Provincial Natural Science Foundation (ZR2019MC060), Key Research and Development Program of Jining City (2018ZDGH024), and a Project of Shandong Province Higher Educational Science and Technology Program (J17KA095).

Abstract

Abstract: Steam pretreatment was employed to disrupt microalgal cells for lipids extraction. Effects of steam pretreatment on microstructure of microalgal cells were investigated through scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Effect of treatment on lipid extraction was also studied. Microalgal cell walls were distorted after steam pretreatment due to the hydrolysis of organic macromolecules contained in cell wall. Maximum curvature was increased from 1.88×10^-6 m^-1 to 1.43×10^-7 m^-1 after treatment with the steam at 130℃. The fractal dimension of microalgal cells increased from 1.25 to 1.30 after pretreatment for 15 min, and further increased to 1.47 when the pretreatment time was increased to 60 min. Increased steam pretreatment temperature and time enhanced the hydrolysis of organic macromolecules, and finally destroyed microalgal cell walls at pretreatment temperature of 130℃ and pretreatment time of 60 min. Lipid extracted from wet microalgal was significantly increased (2.1-fold) after pretreatment.

Key words: Chlorella pyrenoidosa, Steam pretreatment, Cell wall disruption, Lipids extraction

摘要： Steam pretreatment was employed to disrupt microalgal cells for lipids extraction. Effects of steam pretreatment on microstructure of microalgal cells were investigated through scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Effect of treatment on lipid extraction was also studied. Microalgal cell walls were distorted after steam pretreatment due to the hydrolysis of organic macromolecules contained in cell wall. Maximum curvature was increased from 1.88×10^-6 m^-1 to 1.43×10^-7 m^-1 after treatment with the steam at 130℃. The fractal dimension of microalgal cells increased from 1.25 to 1.30 after pretreatment for 15 min, and further increased to 1.47 when the pretreatment time was increased to 60 min. Increased steam pretreatment temperature and time enhanced the hydrolysis of organic macromolecules, and finally destroyed microalgal cell walls at pretreatment temperature of 130℃ and pretreatment time of 60 min. Lipid extracted from wet microalgal was significantly increased (2.1-fold) after pretreatment.

关键词: Chlorella pyrenoidosa, Steam pretreatment, Cell wall disruption, Lipids extraction

Wenlu Song, Rui Huang, Hao Guo, Chunguang Yin, Chuanling Wang, Jun Cheng, Weijuan Yang. Characterization of wet microalgal cells pretreated with steam for lipid extraction[J]. Chinese Journal of Chemical Engineering, 2021, 37(9): 114-120.

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Characterization of wet microalgal cells pretreated with steam for lipid extraction

Characterization of wet microalgal cells pretreated with steam for lipid extraction

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