Preparation and functional study of pH-sensitive amorphous calcium phosphate nanocarriers

doi:10.1016/j.cjche.2021.08.025

Chinese Journal of Chemical Engineering ›› 2022, Vol. 48 ›› Issue (8): 244-252.DOI: 10.1016/j.cjche.2021.08.025

Preparation and functional study of pH-sensitive amorphous calcium phosphate nanocarriers

Baolong Niu^1,2, Min Li^1,2, Jianhong Jia^1,2, Lixuan Ren^1,2, Xin Gang^1,2, Bin Nie^1,2, Yanying Fan⁵, Xiaojie Lian^3,4, Wenfeng Li^1,2

1. College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China;
2. Key Laboratory of Interface Science and Engineering in Advanced Materials, Taiyuan University of Technology, Taiyuan 030024, China;
3. Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China;
4. Shanxi Key Laboratory of Material Strength & Structural Impact, Institute of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China;
5. School of Basic Medical Sciences, Shanxi Medical University, Taiyuan 030001, China

Received:2021-01-23 Revised:2021-08-29 Online:2022-09-30 Published:2022-08-28
Contact: Baolong Niu,E-mail:baolongniutyut@hotmail.com;Wenfeng Li,E-mail:wenfenglityut@live.com
Supported by:
The work was financially supported by the National Natural Science Foundation of China (31700689), Natural Science Foundation of Shanxi Province (201901D111115), Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (172040098-S), and Transformation of Scientific and Technological Achievements Programs of Higher Education Institutions in Shanxi (2020CG015).

Preparation and functional study of pH-sensitive amorphous calcium phosphate nanocarriers

Baolong Niu^1,2, Min Li^1,2, Jianhong Jia^1,2, Lixuan Ren^1,2, Xin Gang^1,2, Bin Nie^1,2, Yanying Fan⁵, Xiaojie Lian^3,4, Wenfeng Li^1,2

1. College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China;
2. Key Laboratory of Interface Science and Engineering in Advanced Materials, Taiyuan University of Technology, Taiyuan 030024, China;
3. Department of Biomedical Engineering, Research Center for Nano-biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China;
4. Shanxi Key Laboratory of Material Strength & Structural Impact, Institute of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China;
5. School of Basic Medical Sciences, Shanxi Medical University, Taiyuan 030001, China

通讯作者: Baolong Niu,E-mail:baolongniutyut@hotmail.com;Wenfeng Li,E-mail:wenfenglityut@live.com
基金资助:
The work was financially supported by the National Natural Science Foundation of China (31700689), Natural Science Foundation of Shanxi Province (201901D111115), Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi (172040098-S), and Transformation of Scientific and Technological Achievements Programs of Higher Education Institutions in Shanxi (2020CG015).

Abstract

Abstract: Recently, multifunctional nanoparticles have shown great prospects in cancer treatment, which have the ability to simultaneously deliver the drug, image and target tumor cells. In this paper, we designed a luminescent nanoparticles platform based on hydrothermal hyaluronic acid/amorphous calcium phosphate (HA-FCNs/ACP) with multifunctional properties for drug delivery, bio-imaging, and targeting treatment. HA-FCNs/ACP shows an ability to load curcumin (Cur) with pH-sensitive responsive drug release behavior and excellent biocompatibility. HA-FCNs/ACP dispersed in the cytoplasm through the overexpressed CD44 receptor that is actively targeted into human lung cancer cells (A549 cells). Meanwhile, the viability of A549 cells was significantly inhibited in vitro. The prepared HA-FCNs and HA-FCNs/ACP both exhibit excellent targeted bioimaging performance on cancer cells. Hence, the as-prepared nanoparticles have promising applications in treating tumor disease.

Key words: Hyaluronic acid, Amorphous calcium phosphate, Fluorescent carbon nanoparticles, pH-sensitive, Tumor targeting

摘要： Recently, multifunctional nanoparticles have shown great prospects in cancer treatment, which have the ability to simultaneously deliver the drug, image and target tumor cells. In this paper, we designed a luminescent nanoparticles platform based on hydrothermal hyaluronic acid/amorphous calcium phosphate (HA-FCNs/ACP) with multifunctional properties for drug delivery, bio-imaging, and targeting treatment. HA-FCNs/ACP shows an ability to load curcumin (Cur) with pH-sensitive responsive drug release behavior and excellent biocompatibility. HA-FCNs/ACP dispersed in the cytoplasm through the overexpressed CD44 receptor that is actively targeted into human lung cancer cells (A549 cells). Meanwhile, the viability of A549 cells was significantly inhibited in vitro. The prepared HA-FCNs and HA-FCNs/ACP both exhibit excellent targeted bioimaging performance on cancer cells. Hence, the as-prepared nanoparticles have promising applications in treating tumor disease.

关键词: Hyaluronic acid, Amorphous calcium phosphate, Fluorescent carbon nanoparticles, pH-sensitive, Tumor targeting

Baolong Niu, Min Li, Jianhong Jia, Lixuan Ren, Xin Gang, Bin Nie, Yanying Fan, Xiaojie Lian, Wenfeng Li. Preparation and functional study of pH-sensitive amorphous calcium phosphate nanocarriers[J]. Chinese Journal of Chemical Engineering, 2022, 48(8): 244-252.

References

[1] C. Minelli, S.B. Lowe, M.M. Stevens, Engineering nanocomposite materials for cancer therapy, Small 6 (21) (2010) 2336–2357
[2] D. Wang, J.F. Chen, L.M. Dai, Recent advances in graphene quantum dots for fluorescence bioimaging from cells through tissues to animals, Part. Part. Syst. Charact. 32 (5) (2015) 515–523
[3] A. Konwar, D. Chowdhury, Property relationship of alginate and alginate–carbon dot nanocomposites with bivalent and trivalent cross-linker ions, RSC Adv. 5 (77) (2015) 62864–62870
[4] C.J. Jeong, A.K. Roy, S.H. Kim, J.E. Lee, J.H. Jeong, I. In, S.Y. Park, Fluorescent carbon nanoparticles derived from natural materials of mango fruit for bio-imaging probes, Nanoscale 6 (24) (2014) 15196–15202
[5] W. Hyung, H. Ko, J. Park, E. Lim, S.B. Park, Y.J. Park, H.G. Yoon, J.S. Suh, S. Haam, Y.M. Huh, Novel hyaluronic acid (HA) coated drug carriers (HCDCs) for human breast cancer treatment, Biotechnol Bioeng 99 (2) (2008) 442–454
[6] G.M. Son, H.Y. Kim, J.H. Ryu, C.W. Chu, D.H. Kang, S.B. Park, Y.I. Jeong, Self-assembled polymeric micelles based on hyaluronic acid-g-poly(D, L-lactide-co-glycolide) copolymer for tumor targeting, Int J Mol Sci 15 (9) (2014) 16057–16068
[7] M. Yazan, I.D. Kocyigit, F. Atil, U. Tekin, Z.B. Gonen, M.E. Onder, Effect of hyaluronic acid on the osseointegration of dental implants, Br J Oral Maxillofac Surg 57 (1) (2019) 53–57
[8] Fakhari A, Berkland C, Applications and emerging trends of hyaluronic acid in tissue engineering, as a dermal filler and in osteoarthritis treatment, Acta Biomater 9 (7) (2013) 7081–7092
[9] G. Jose, Y.J. Lu, H.A. Chen, H.L. Hsu, J.T. Hung, T.S. Anilkumar, J.P. Chen, Hyaluronic acid modified bubble-generating magnetic liposomes for targeted delivery of doxorubicin, J. Magn. Magn. Mater. 474 (2019) 355–364
[10] J.P. Quinones, J. Jokinen, S. Kein?nen, C.P. Covas, O. Brüggemann, D. Ossipov, Self-assembled hyaluronic acid-testosterone nanocarriers for delivery of anticancer drugs, Eur. Polym. J. 99 (2018) 384–393
[11] W.Y. Gui, J.R. Zhang, X.Q. Chen, D.H. Yu, Q. Ma, N-Doped graphene quantum dot@mesoporous silica nanoparticles modified with hyaluronic acid for fluorescent imaging of tumor cells and drug delivery, Mikrochim Acta 185 (1) (2017) 66
[12] Z. Zyman, M. Epple, A. Goncharenko, D. Rokhmistrov, O. Prymak, K. Loza, Thermally induced crystallization and phase evolution in powders derived from amorphous calcium phosphate precipitates with a Ca/P ratio of 1: 1, J. Cryst. Growth 450 (2016) 190–196
[13] Q.Z. Chen, C.T. Wong, W.W. Lu, K.M. Cheung, J.C. Leong, K.D. Luk, Strengthening mechanisms of bone bonding to crystalline hydroxyapatite in vivo, Biomaterials 25 (18) (2004) 4243–4254
[14] N. Rangavittal, A.R. Landa-Cánovas, J.M. González-Calbet, M. Vallet-Regí, Structural study and stability of hydroxyapatite and beta-tricalcium phosphate: Two important bioceramics. Journal of Biomedical Materials Research 51(4) (2015) 660–668
[15] C. Qi, Y.J. Zhu, B.Q. Lu, X.Y. Zhao, J. Zhao, F. Chen, Hydroxyapatite nanosheet-assembled porous hollow microspheres: DNA-templated hydrothermal synthesis, drug delivery and protein adsorption, J. Mater. Chem. 22 (42) (2012) 22642
[16] M.P. Ginebra, T. Traykova, J.A. Planell, Calcium phosphate cements as bone drug delivery systems: A review, J Control Release 113 (2) (2006) 102–110
[17] X.Y. Zhao, Y.J. Zhu, F. Chen, B.Q. Lu, J. Wu, Nanosheet-assembled hierarchical nanostructures of hydroxyapatite: Surfactant-free microwave-hydrothermal rapid synthesis, protein/DNA adsorption and pH-controlled release, CrystEngComm 15 (1) (2013) 206–212
[18] F. Chen, P. Huang, C. Qi, B.Q. Lu, X.Y. Zhao, C. Li, J. Wu, D.X. Cui, Y.J. Zhu, Multifunctional biodegradable mesoporous microspheres of Eu3+-doped amorphous calcium phosphate: Microwave-assisted preparation, pH-sensitive drug release, and bioimaging application, J Mater Chem B 2 (41) (2014) 7132–7140
[19] X. Guo, W.F. Li, H.P. Wang, Y.Y. Fan, H.F. Wang, X.H. Gao, B.L. Niu, X.C. Gong, Preparation, characterization, release and antioxidant activity of curcumin-loaded amorphous calcium phosphate nanoparticles, J. Non - Cryst. Solids 500 (2018) 317–325
[20] L. Chen, H.L. Zhu, S. Yang, B.B. Zhou, F.H. You, X.M. Yan, Nanostructured calcium phosphate carriers for deliver of poor water-soluble drug silybin, Mater. Lett. 143 (2015) 252–255
[21] Y.Q. Zhang, Y.S. Hu, J. Lin, Y. Fan, Y.T. Li, Y. Lv, X.Y. Liu, Excitation wavelength independence: Toward low-threshold amplified spontaneous emission from carbon nanodots, ACS Appl Mater Interfaces 8 (38) (2016) 25454–25460
[22] C. Saikia, M.K. Das, A. Ramteke, T.K. Maji, Effect of crosslinker on drug delivery properties of curcumin loaded starch coated iron oxide nanoparticles, Int J Biol Macromol 93 (Pt A) (2016) 1121–1132
[23] M.D. Massich, D.A. Giljohann, A.L. Schmucker, P.C. Patel, C.A. Mirkin, Cellular response of polyvalent oligonucleotide-gold nanoparticle conjugates, ACS Nano 4 (10) (2010) 5641–5646
[24] G.J. Ding, Y.J. Zhu, C. Qi, B.Q. Lu, J. Wu, F. Chen, Porous microspheres of amorphous calcium phosphate: Block copolymer templated microwave-assisted hydrothermal synthesis and application in drug delivery, J Colloid Interface Sci 443 (2015) 72–79
[25] D. Pasqui, P. Torricelli, M. De Cagna, M. Fini, R. Barbucci, Carboxymethyl cellulose-hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications, J Biomed Mater Res A 102 (5) (2014) 1568–1579
[26] C. Qi, Y.J. Zhu, X.Y. Zhao, J. Zhao, F. Chen, G.F. Cheng, Y.J. Ruan, High surface area carbonate apatite nanorod bundles: Surfactant-free sonochemical synthesis and drug loading and release properties, Mater. Res. Bull. 48 (4) (2013) 1536–1540
[27] I.R. Gibson, W. Bonfield, Novel synthesis and characterization of an AB-type carbonate-substituted hydroxyapatite, J. Biomed. Mater. Res. 59 (4) (2002) 697–708
[28] B.J.M. Leite Ferreira, M.C.F. Magalh?es, R.N. Correia, In vitro formation of apatites and other biologically related calcium phosphates: Influence of temperature and pH on the nature of the mineral phases, Mater. Sci. Forum 587-588 (2008) 7–11

Preparation and functional study of pH-sensitive amorphous calcium phosphate nanocarriers

Preparation and functional study of pH-sensitive amorphous calcium phosphate nanocarriers

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 0

Recommended Articles

Metrics

Comments