Assessing quinoline removal performances of an aerobic continuous moving bed biofilm reactor (MBBR) bioaugmented with Pseudomonas citronellolis LV1

doi:10.1016/j.cjche.2022.09.009

Chinese Journal of Chemical Engineering ›› 2023, Vol. 57 ›› Issue (5): 132-140.DOI: 10.1016/j.cjche.2022.09.009

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Assessing quinoline removal performances of an aerobic continuous moving bed biofilm reactor (MBBR) bioaugmented with Pseudomonas citronellolis LV1

Hu Chen^1,3, Ying Wang², Puyu Wang², Yongkang Lv^1,2,4

1. Innovation Institute of Environmental Industry, College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China;
2. The State Key Laboratory of Clean and Efficient Utilization of Coal-Based Energy, Taiyuan University of Technology, Taiyuan 030024, China;
3. China Institute for Radiation Protection, Taiyuan 030006, China;
4. Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan 030024, China

Received:2022-06-23 Revised:2022-09-16 Online:2023-07-08 Published:2023-05-28
Contact: Ying Wang,E-mail:wangying0617@126.com;Yongkang Lv,E-mail:yongkanglv@163.com
Supported by:
This work was financially supported by the Basic Research Project for Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering (2021SX-AT004), the Shanxi Province Science Foundation for Youths (20210302124348, 202103021223099), and the National Natural Science Foundation of China (51778397).

Assessing quinoline removal performances of an aerobic continuous moving bed biofilm reactor (MBBR) bioaugmented with Pseudomonas citronellolis LV1

Hu Chen^1,3, Ying Wang², Puyu Wang², Yongkang Lv^1,2,4

1. Innovation Institute of Environmental Industry, College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China;
2. The State Key Laboratory of Clean and Efficient Utilization of Coal-Based Energy, Taiyuan University of Technology, Taiyuan 030024, China;
3. China Institute for Radiation Protection, Taiyuan 030006, China;
4. Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan 030024, China

通讯作者: Ying Wang,E-mail:wangying0617@126.com;Yongkang Lv,E-mail:yongkanglv@163.com
基金资助:
This work was financially supported by the Basic Research Project for Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering (2021SX-AT004), the Shanxi Province Science Foundation for Youths (20210302124348, 202103021223099), and the National Natural Science Foundation of China (51778397).

Abstract

Abstract: This study evaluated the bioaugmentation potential of a quinoline-degrading strain Pseudomonas citronellolis LV1 inoculation into activated sludge for treating quinoline wastewater, and results indicated the inoculation of LV1 in aerobic continuous MBBR could substantially improve the quinoline removal performance with an improved removal efficiency of 34% averagely when quinoline was used as the sole carbon and nitrogen source. Additionally, efficient removal of quinoline in enhanced MBBR occurred at the influent pH of 7.0–8.0, hydraulic retention time (HRT) of 24–28 h and influent quinoline concentration of 100–700 mg·L^-1. High-throughput sequencing analysis indicated that bioaugmentation could increase microbial diversity and shape the microbial community structure. Although the inoculant LV1 did not remain its dominance in stage III, bioaugmentation indeed induced the formation of effective microbial community, and the indigenous microbes including Flavobacterium, Pseudoxanthomonas, Pseudomonas, Vermamoeba, Dyadobacter and Sphingomonas might play the key role in quinoline removal. According to the PICRUSt, the enhanced genes encoding aromatic ring-cleavage enzyme, especially for N-heterocyclic ring-cleavage enzymes, could lead to the improved removal performance of quinoline in bioaugmentation stage. Moreover, the enhanced MBBR treated well actual coking wastewater, as indicated by high removal performance of quinoline, phenol and COD.

Key words: Aerobic quinoline degradation, Pseudomonas citronellolis LV1, Bioaugmentation, Microbial community, Coking wastewater

摘要： This study evaluated the bioaugmentation potential of a quinoline-degrading strain Pseudomonas citronellolis LV1 inoculation into activated sludge for treating quinoline wastewater, and results indicated the inoculation of LV1 in aerobic continuous MBBR could substantially improve the quinoline removal performance with an improved removal efficiency of 34% averagely when quinoline was used as the sole carbon and nitrogen source. Additionally, efficient removal of quinoline in enhanced MBBR occurred at the influent pH of 7.0–8.0, hydraulic retention time (HRT) of 24–28 h and influent quinoline concentration of 100–700 mg·L^-1. High-throughput sequencing analysis indicated that bioaugmentation could increase microbial diversity and shape the microbial community structure. Although the inoculant LV1 did not remain its dominance in stage III, bioaugmentation indeed induced the formation of effective microbial community, and the indigenous microbes including Flavobacterium, Pseudoxanthomonas, Pseudomonas, Vermamoeba, Dyadobacter and Sphingomonas might play the key role in quinoline removal. According to the PICRUSt, the enhanced genes encoding aromatic ring-cleavage enzyme, especially for N-heterocyclic ring-cleavage enzymes, could lead to the improved removal performance of quinoline in bioaugmentation stage. Moreover, the enhanced MBBR treated well actual coking wastewater, as indicated by high removal performance of quinoline, phenol and COD.

关键词: Aerobic quinoline degradation, Pseudomonas citronellolis LV1, Bioaugmentation, Microbial community, Coking wastewater

Hu Chen, Ying Wang, Puyu Wang, Yongkang Lv. Assessing quinoline removal performances of an aerobic continuous moving bed biofilm reactor (MBBR) bioaugmented with Pseudomonas citronellolis LV1[J]. Chinese Journal of Chemical Engineering, 2023, 57(5): 132-140.

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Assessing quinoline removal performances of an aerobic continuous moving bed biofilm reactor (MBBR) bioaugmented with Pseudomonas citronellolis LV1

Assessing quinoline removal performances of an aerobic continuous moving bed biofilm reactor (MBBR) bioaugmented with Pseudomonas citronellolis LV1

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