Towards understanding the mechanism of n-hexane tolerance in Synechocystis sp. PCC 6803

doi:10.1016/j.cjche.2022.11.015

Chinese Journal of Chemical Engineering ›› 2023, Vol. 59 ›› Issue (7): 128-134.DOI: 10.1016/j.cjche.2022.11.015

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Towards understanding the mechanism of n-hexane tolerance in Synechocystis sp. PCC 6803

Tao Sun^1,2,3, Shubin Li^1,2, Guangsheng Pei^1,2, Lei Chen^1,2, Weiwen Zhang^1,2,3

1. Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China;
2. Frontier Science Center for Synthetic Biology & Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, China;
3. Center for Biosafety Research and Strategy, Tianjin University, Tianjin 300072, China

Received:2022-10-22 Revised:2022-11-18 Online:2023-10-14 Published:2023-07-28
Contact: Lei Chen,E-mail:lchen@tju.edu.cn;Weiwen Zhang,E-mail:wwzhang8@tju.edu.cn
Supported by:
This research was supported by grants from the National Key Research and Development Program of China (2020YFA0906800, 2021YFA0909700, 2018YFA0903600 and 2019YFA0904600).

Towards understanding the mechanism of n-hexane tolerance in Synechocystis sp. PCC 6803

Tao Sun^1,2,3, Shubin Li^1,2, Guangsheng Pei^1,2, Lei Chen^1,2, Weiwen Zhang^1,2,3

1. Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China;
2. Frontier Science Center for Synthetic Biology & Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin 300072, China;
3. Center for Biosafety Research and Strategy, Tianjin University, Tianjin 300072, China

通讯作者: Lei Chen,E-mail:lchen@tju.edu.cn;Weiwen Zhang,E-mail:wwzhang8@tju.edu.cn
基金资助:
This research was supported by grants from the National Key Research and Development Program of China (2020YFA0906800, 2021YFA0909700, 2018YFA0903600 and 2019YFA0904600).

Abstract

Abstract: Synthetic biology efforts have also led to the development of photosynthetic cyanobacteria as “autotrophic cell factories” for biosynthesis of various biofuels directly from CO₂. However, the low tolerance to toxicity of biofuels has restricted the economic application of cyanobacterial hosts. In this study, RNA-seq transcriptomics was employed to reveal stress responses to exogenous n-hexane in Synechocystis sp. PCC 6803. Functional enrichment analysis of the transcriptomic data showed that signal transduction systems were induced significantly. To further identify regulatory genes related to n-hexane tolerance, a library of transcriptional regulators (TRs) deletion mutants was then screened for their roles in n-hexane tolerance. The results showed that a knockout mutant of slr0724 that encodes an HtaR suppressor protein was more tolerant to n-hexane than the wild type, indicating the involvement of slr0724 in n-hexane tolerance. This study provides the foundation for better understanding the cellular responses to n-hexane in Synechocystis sp. PCC 6803, which could contribute to the further engineering of n-hexane tolerance in cyanobacteria.

Key words: Transcriptome, n-Hexane tolerance, Metabolomics, Synechocystis

摘要： Synthetic biology efforts have also led to the development of photosynthetic cyanobacteria as “autotrophic cell factories” for biosynthesis of various biofuels directly from CO₂. However, the low tolerance to toxicity of biofuels has restricted the economic application of cyanobacterial hosts. In this study, RNA-seq transcriptomics was employed to reveal stress responses to exogenous n-hexane in Synechocystis sp. PCC 6803. Functional enrichment analysis of the transcriptomic data showed that signal transduction systems were induced significantly. To further identify regulatory genes related to n-hexane tolerance, a library of transcriptional regulators (TRs) deletion mutants was then screened for their roles in n-hexane tolerance. The results showed that a knockout mutant of slr0724 that encodes an HtaR suppressor protein was more tolerant to n-hexane than the wild type, indicating the involvement of slr0724 in n-hexane tolerance. This study provides the foundation for better understanding the cellular responses to n-hexane in Synechocystis sp. PCC 6803, which could contribute to the further engineering of n-hexane tolerance in cyanobacteria.

关键词: Transcriptome, n-Hexane tolerance, Metabolomics, Synechocystis

Tao Sun, Shubin Li, Guangsheng Pei, Lei Chen, Weiwen Zhang. Towards understanding the mechanism of n-hexane tolerance in Synechocystis sp. PCC 6803[J]. Chinese Journal of Chemical Engineering, 2023, 59(7): 128-134.

Tao Sun, Shubin Li, Guangsheng Pei, Lei Chen, Weiwen Zhang. Towards understanding the mechanism of n-hexane tolerance in Synechocystis sp. PCC 6803[J]. 中国化学工程学报, 2023, 59(7): 128-134.

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Towards understanding the mechanism of n-hexane tolerance in Synechocystis sp. PCC 6803

Towards understanding the mechanism of n-hexane tolerance in Synechocystis sp. PCC 6803

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