Abstract: The chemical synthesis of Guanine arabinoside(ara-G)is extremely complex,time-consuming,and seriously polluted.A two-step enzymatic synthesis process was developed to acquire ara-G easily.2,6-Diaminopurine arabinoside(ara-DA)was first synthesized with purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase produced by Enterobacter aerogenes DGW-07.The conversion yield of ara-DA could reach above 90% when the reaction liquid contained 30 mmol·L^-1 uracil arabinoside as arabinose donor,10 mmol·L^-1 2,6-diaminopurine as arabinose acceptor in pH7.0 20 mmol·L^-1 phosphate buffer,and reacted at 60℃ for 48h.Then,ara-DA was effectively transformed into ara-G with adenylate deaminase produced by Aspergillus oryzae DAW-01.The total process had no complex separation and purification.

Key words: guanine arabinoside, 2,6-diaminopurine arabinoside, purine nucleoside phosphorylase, pyrimidine nucleoside phosphorylase, adenylate deaminase, Enterobacter aerogenes, Aspergillus oryzae

摘要： The chemical synthesis of Guanine arabinoside(ara-G)is extremely complex,time-consuming,and seriously polluted.A two-step enzymatic synthesis process was developed to acquire ara-G easily.2,6-Diaminopurine arabinoside(ara-DA)was first synthesized with purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase produced by Enterobacter aerogenes DGW-07.The conversion yield of ara-DA could reach above 90% when the reaction liquid contained 30 mmol·L^-1 uracil arabinoside as arabinose donor,10 mmol·L^-1 2,6-diaminopurine as arabinose acceptor in pH7.0 20 mmol·L^-1 phosphate buffer,and reacted at 60℃ for 48h.Then,ara-DA was effectively transformed into ara-G with adenylate deaminase produced by Aspergillus oryzae DAW-01.The total process had no complex separation and purification.

关键词: guanine arabinoside, 2,6-diaminopurine arabinoside, purine nucleoside phosphorylase, pyrimidine nucleoside phosphorylase, adenylate deaminase, Enterobacter aerogenes, Aspergillus oryzae