Influence of L-Cysteine Concentration on Oxidation-reduction Potential and Biohydrogen Production

Abstract

Abstract: The effects of L-cysteine concentration on biohydrogen production by Enterobacterium Bacterium M580 were investigated in batch cultivation.The experimental results showed that L-cysteine could enhance the cell growth,hydrogen production rate and hydrogen yield when its concentration was less than 500 mg·L^-1,while it had negative effects when its concentration was higher than 500 mg·L^-1.The hydrogen production was the highest 1.29 mol·mol^-1(H₂/glucose) when 300 mg·L^-1 L-cysteine was added into the culture,and the yield was 9.4% higher than that in the control.The oxidation-reduction potential(ORP),which was influenced by L-cysteine,also affected hydrogen production.The ORP values were in the range -300 mV to -150 mV when the L-cysteine concentration was higher than 500 mg·L^-1.Although the ORP in this range was favorable for hydrogen production,it was not suitable for the biomass growth.Hence,less hydrogen was produced.When the L-cysteine concentration was lower than 500 mg·L^-1,the ORP was more suitable for both biomass growth and hydrogen production.In addition,at least 91%glucose was consumed when L-cysteine was added to the culture media,compared to the 97.37% consumption without L-cysteine added.

Key words: anaerobic fermentation, biohydrogen, L-cysteine, ORP

摘要： The effects of L-cysteine concentration on biohydrogen production by Enterobacterium Bacterium M580 were investigated in batch cultivation.The experimental results showed that L-cysteine could enhance the cell growth,hydrogen production rate and hydrogen yield when its concentration was less than 500 mg·L^-1,while it had negative effects when its concentration was higher than 500 mg·L^-1.The hydrogen production was the highest 1.29 mol·mol^-1(H₂/glucose) when 300 mg·L^-1 L-cysteine was added into the culture,and the yield was 9.4% higher than that in the control.The oxidation-reduction potential(ORP),which was influenced by L-cysteine,also affected hydrogen production.The ORP values were in the range -300 mV to -150 mV when the L-cysteine concentration was higher than 500 mg·L^-1.Although the ORP in this range was favorable for hydrogen production,it was not suitable for the biomass growth.Hence,less hydrogen was produced.When the L-cysteine concentration was lower than 500 mg·L^-1,the ORP was more suitable for both biomass growth and hydrogen production.In addition,at least 91%glucose was consumed when L-cysteine was added to the culture media,compared to the 97.37% consumption without L-cysteine added.

关键词: anaerobic fermentation, biohydrogen, L-cysteine, ORP

CHEN Huoqing, MA Xiaoxuan, FAN Daidi, LUO Yan'e, GAO Pengfei, YANG Chanyuan. Influence of L-Cysteine Concentration on Oxidation-reduction Potential and Biohydrogen Production[J]. , 2010, 18(4): 681-686.

陈火晴, 马晓轩, 范代娣, 骆艳娥, 高鹏飞, 杨婵媛. Influence of L-Cysteine Concentration on Oxidation-reduction Potential and Biohydrogen Production[J]. , 2010, 18(4): 681-686.

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