Isolation and Purification of Triptolide from the Leaves of Tripterygium wilfordii Hook F

›› 2010, Vol. 18 ›› Issue (5): 750-754.

• SEPARATION SCIENCE AND ENGINEERING • Previous Articles Next Articles

Isolation and Purification of Triptolide from the Leaves of Tripterygium wilfordii Hook F

SHEN Jian, HE Chaohong

State Key Laboratory of Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang Uni versity, Hangzhou 310027, China

Received:2010-01-28 Revised:2010-06-15 Online:2010-10-28 Published:2010-10-28
Supported by:
Supported by the National Natural Science Foundation of China (20576113);Zhejiang Provincial Natural Science Foundation of China (R4090358)

Isolation and Purification of Triptolide from the Leaves of Tripterygium wilfordii Hook F

沈剑, 何潮洪

State Key Laboratory of Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang Uni versity, Hangzhou 310027, China

通讯作者: HE Chaohong,E-mail:chhezju@zju.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China (20576113);Zhejiang Provincial Natural Science Foundation of China (R4090358)

Abstract

Abstract: Triptolide is an important active component of Tripterygium wilfordii Hook F (TWHF) and possesses anti-inflammatory, immunosuppressive, male anti-fertility, and anticancer properties. A new method combining different techniques, including solid-liquid extraction, liquid-liquid partition, column chromatography and high-speed counter-current chromatography (HSCCC) but avoiding the use of chloroform, was developed for the isolation and purification of triptolide from the leaves of TWHF. 48 mg of triptolide at 96.5% purity was obtained from 1 kg of air-dried leaves of TWHF.

Key words: Tripterygium wilfordii Hook F, triptolide, high-speed counter-current chromatography

摘要： Triptolide is an important active component of Tripterygium wilfordii Hook F (TWHF) and possesses anti-inflammatory, immunosuppressive, male anti-fertility, and anticancer properties. A new method combining different techniques, including solid-liquid extraction, liquid-liquid partition, column chromatography and high-speed counter-current chromatography (HSCCC) but avoiding the use of chloroform, was developed for the isolation and purification of triptolide from the leaves of TWHF. 48 mg of triptolide at 96.5% purity was obtained from 1 kg of air-dried leaves of TWHF.

关键词: Tripterygium wilfordii Hook F, triptolide, high-speed counter-current chromatography

SHEN Jian, HE Chaohong. Isolation and Purification of Triptolide from the Leaves of Tripterygium wilfordii Hook F[J]. , 2010, 18(5): 750-754.

沈剑, 何潮洪. Isolation and Purification of Triptolide from the Leaves of Tripterygium wilfordii Hook F[J]. , 2010, 18(5): 750-754.

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Isolation and Purification of Triptolide from the Leaves of Tripterygium wilfordii Hook F

Isolation and Purification of Triptolide from the Leaves of Tripterygium wilfordii Hook F

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