Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process

›› 2011, Vol. 19 ›› Issue (6): 1023-1027.

• BIOTECHNOLOGY AND BIOENGINEERING • Previous Articles Next Articles

Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process

CHEN Guo, YANG Daomao, XIAO Yaqin, CHEN Hongwen

Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, China

Received:2010-09-20 Revised:2011-08-18 Online:2012-04-24 Published:2011-12-28
Supported by:
Supported by the National Natural Science Foundation of China (20906035);the Fundamental Research Funds for the Central Universities (JB-JX1002)

Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process

陈国, 杨道茂, 肖雅琴, 陈宏文

Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, China

通讯作者: CHEN Guo, E-mail: chenguo@hqu.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China (20906035);the Fundamental Research Funds for the Central Universities (JB-JX1002)

Abstract

Abstract: 3-Hydroxypropionaldehyde (3-HPA) is a potential valuable chemical and new broad-spectrum antimicrobial substance. In order to improve the conversion of 3-HPA/glycerol, our work studied the two-step process from glycerol to 3-HPA, and investigated the influence of cell harvest time, glycerol concentration, biomass con-centration, pH and temperature on the production of 3-HPA by Lactobacillus reuteri CG001, respectively. The results showed that molar conversion yield of 3-HPA/glycerol reached 97.9% under the condition that 200 mmol·L^-1 glycerol was converted by 25.3 g·L^-1 resting cell for 1 h at 30℃. The cells could not be reused directly because the L. reuteri almost lost its bioconversion activity completely, but the ability of glycerol conversion could gradually recover if the fresh medium was added to the deactivated cell for 4 h.

Key words: Lactobacillus reuteri, 3-hydroxypropionaldehyde, reuterin, bioconversion

摘要： 3-Hydroxypropionaldehyde (3-HPA) is a potential valuable chemical and new broad-spectrum antimicrobial substance. In order to improve the conversion of 3-HPA/glycerol, our work studied the two-step process from glycerol to 3-HPA, and investigated the influence of cell harvest time, glycerol concentration, biomass con-centration, pH and temperature on the production of 3-HPA by Lactobacillus reuteri CG001, respectively. The results showed that molar conversion yield of 3-HPA/glycerol reached 97.9% under the condition that 200 mmol·L^-1 glycerol was converted by 25.3 g·L^-1 resting cell for 1 h at 30℃. The cells could not be reused directly because the L. reuteri almost lost its bioconversion activity completely, but the ability of glycerol conversion could gradually recover if the fresh medium was added to the deactivated cell for 4 h.

关键词: Lactobacillus reuteri, 3-hydroxypropionaldehyde, reuterin, bioconversion

CHEN Guo, YANG Daomao, XIAO Yaqin, CHEN Hongwen. Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process[J]. , 2011, 19(6): 1023-1027.

陈国, 杨道茂, 肖雅琴, 陈宏文. Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process[J]. , 2011, 19(6): 1023-1027.

References

1 Jones, S.E., Versalovic, J., “Probiotic Lactobacillus reuteri biofilms produce antimicrobial and anti-inflammatory factors”, BMC Microbiology, 9, 35-44(2009).
2 Cleusix, V., Lacroix, C., Vollenweider, S., “Inhibitory activity spectrum of reuterin produced by Lactobacillus reuteri against intestinal bacteria”, BMC Microbiology, 7, 101-109(2007).
3 Talarico, T., Dobrogosz, W., “Chemical characterization of an antimicrobial substance produced by Lactobacillus reuteri”, Antimicrob. Agents Chemother., 33(5), 674-679(1989).
4 Vollenweider, S., Grassi, G., Iwo, K., Puhan, Z., “Purification and structural characterization of 3-hydroxypropionaldehyde and its derivatives”, J. Agric. Food Chem., 51(11), 3287-3293(2003).
5 Axelsson, L.T., Chung, T.C., Dobrogosz, W.J., Lindgren, S.E., “Procuction of a broad spectrum antimicrobial substance by Lactobacillus reuteri”, Microb. Ecol. Health Dis., 2(2), 131-136(1989).
6 El-Ziney, M.G., Debevere, J.M., “The effect of reuterin on Listeria monocytogenes and Escherichia coli O157:H7 in milk and cottage cheese”, J. Food Prot., 61(10), 1275-1280(1998).
7 El-Ziney, M.G., vanden Tempel, T., Debevere, J., Jakobsen, M., “Application of reuterin produced by Lactobacillus reuteri 12002 for meat decontamination and preservation”, J. Food Prot., 62(3), 257-261(1999).
8 Ruiz-Moyano, S., Martin, A., Benito, M.J., “Safety and functional aspects of pre-selected lactobacilli for probiotic use in Iberian dry-fermented sausages”, Meat Science, 83(3), 460-467(2009).
9 McQuestin, O.J., Shadbolt, C.T., Ross, T., “Quantification of the relative effects of temperature, pH, and water activity on inactivation of Escherichia coli in fermented meat by meta-analysis”, Appl. Environ. Microbiol., 75(22), 6963-6972(2009).
10 Spinler, J.K., Taweechotipatr, M., Rognerud, C.L., Ou, C.N., Tumwasorn, S., Versalovic, J., “Human-derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens”, Anaerobe, 14(3), 166-171(2008).
11 El-Ziney, M.G., Jakobsen, M., “Efectiveness of reuterin alone and in combination with nisin or other food contact surfaces sanitizers and cleaners for disinfection of stainless steel surfaces contaminated with Escherichia coli and Listeria innocua”, J. Food Agri. & Environ., 7(1), 145-149(2009).
12 Talarico, T.L., Dobrogosz, W.J., “Purification and characterization of glycerol dehydratase from Lactobacillus reuteri”, Appl. Environ. Microbiol., 56(4), 1195-1197(1990).
13 Barbirato, F., Grivet, J.P., Soucaille, P., Bories, A., “3-Hydroxypropionaldehyde, an inhibitory metabolite of glycerol fermentation to 1,3-propanediol by enterobacterial species”, Appl. Environ. Microbial., 62(4), 1448-1451(1996).
14 Montserrat, T., Angel, F.M., José, A.C., Juan, J.R., “Unstructured kinetic model for reuterin and 1,3-propanediol production by Lactobacillus reuteri from glycerol/glucose cofermentation”, J. Chem. Technol. Biotechnol., 84(5), 675-680(2009).
15 Talarico, T.L., Casas, I.A., Chung, T.C., Dobrogosz, W.J., “Production and isolation of reuterin, a growth inhibitor produced by Lactobacillus reuteri”, Antimicrob. Agents Chemother., 32(12), 1854-1858(1988).
16 Lüthi-Peng, Q., Sch rer, S., Puhan, Z., “Production and stability of 3-hydroxypropionaldehyde in Lactobacillus reuteri”, Appl. Microbiol. Biotechnol., 60(1-2), 73-80(2002).
17 Lüthi-Peng, Q., Dileme, F.B.S., Puhan, Z., “Effect of glucose on glycerol bioconversion by Lactobacillus ruteri”, Appl. Microbiol. Biotechnol., 59(2-3), 289-296(2002).
18 Doleyres, Y., Beck, P., Vollenweider, S., Lacroix, C., “Production of 3-hydroxypiopionaldehyde using a two-step process with Lactobacillus reuteri”, Appl. Microbiol. Biotechnol., 68(4), 467-474(2005).
19 Fabien, B., Philippe, S., Carole, C., Andréb, B., “Uncoupled glycerol distribution as the origin of the accumulation of 3-hydroxypropionaldehyde during the fermentation of glycerol by Enterobacter agglomerans CNCM 1210”, Biotechnol. Bioeng., 58(2-3), 303-305(1998).
20 Kajiura, H., Mori, K., Shibata, N., Toraya, T., “Molecular basis for specificities of reactivating factors for adenosylcobalamin-dependent diol and glycerol dehydratases”, FEBS J., 274(21), 5556-5566(2007).
21 Talarico, T.L., Axelsson, L.T., Novotny, J., Fiuzat, M., Dobrogosz, W.J., “Utilization of glycerol as a hydrogen acceptor by Lactobacillus reuteri: purification of 1,3-propanediol: NAD+ oxidoreductase”, Appl. Environ. Microbiol., 56(4), 943-948(1990).

[1]	Siyuan Gao, Yuanke Guo, Chen Ma, Ding Ma, Kequan Chen, Pingkai Ouyang, Xin Wang. Characterization and application of a recombinant dopa decarboxylase from Harmonia axyridis for the efficient biosynthesis of dopamine [J]. Chinese Journal of Chemical Engineering, 2022, 41(1): 449-456.
[2]	Han Zhang, Yunpeng Bai, Ning Zhu, Jianhe Xu. Microfluidic reactor with immobilized enzyme-from construction to applications: A review [J]. Chinese Journal of Chemical Engineering, 2021, 29(2): 136-145.
[3]	Chen Ma, Jing Wang, Xuelin Wang, Dandan Mai, Yuqi Jin, Kequan Chen, Xin Wang, Pingkai Ouyang. Efficient production of cyclic adenosine monophosphate from adenosine triphosphate by the N-terminal half of adenylate cyclase from Escherichia coli [J]. Chinese Journal of Chemical Engineering, 2020, 28(8): 2167-2172.

Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process

Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics

Comments