Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids

Abstract

Abstract: Functionalized ionic liquids containing ethyoxyl groups were synthesized and immobilized on magnetic silica nanoparticles(MSNP) prepared by two steps,i.e.,Fe₃O₄ synthesis and silica shell growth on the surface.This magnetic nanoparticle supported ionic liquid(MNP-IL) were applied in the immobilization of penicillin G acylase(PGA).The MSNPs and MNP-ILs were characterized by the means of Fourier transform infrared spectroscopy(FTIR),scanning electron microscopy(SEM),transmission electron microscopy(TEM),and vibrating sample magnetometer(VSM).The results showed that the average size of magnetic Fe₃O₄ nanoparticles and MSNPs were ~10 and ~90 nm,respectively.The saturation magnetizations of magnetic Fe₃O₄ nanoparticles and MNP-ILs were 63.7 and 26.9 A·m²·kg^-1,respectively.The MNP-IL was successfully applied in the immobilization of PGA.The maximum amount of loaded enzyme was about 209 mg·g^-1(based on carrier),and the highest enzyme activity of immobilized PGA(based on ImPGA) was 261 U·g^-1.Both the amount of loaded enzyme and the activity of ImPGA are at the same level of or higher than that in previous reports.After 10 consecutive operations,ImPGA still maintained 62% of its initial activity,indicating the good recovery property of ImPGA activity.The ionic liquid modified magnetic particles integrate the magnetic properties of Fe₃O₄ and the structure-tunable properties of ionic liquids,and have extensive potential uses in protein immobilization and magnetic bioseparation.This work may open up a novel strategy to immobilize proteins by ionic liquids.

Key words: magnetic iron oxide, magnetic nanoparticles, ionic liquids, penicillin G acylase, enzyme immobilization

摘要： Functionalized ionic liquids containing ethyoxyl groups were synthesized and immobilized on magnetic silica nanoparticles(MSNP) prepared by two steps,i.e.,Fe₃O₄ synthesis and silica shell growth on the surface.This magnetic nanoparticle supported ionic liquid(MNP-IL) were applied in the immobilization of penicillin G acylase(PGA).The MSNPs and MNP-ILs were characterized by the means of Fourier transform infrared spectroscopy(FTIR),scanning electron microscopy(SEM),transmission electron microscopy(TEM),and vibrating sample magnetometer(VSM).The results showed that the average size of magnetic Fe₃O₄ nanoparticles and MSNPs were ~10 and ~90 nm,respectively.The saturation magnetizations of magnetic Fe₃O₄ nanoparticles and MNP-ILs were 63.7 and 26.9 A·m²·kg^-1,respectively.The MNP-IL was successfully applied in the immobilization of PGA.The maximum amount of loaded enzyme was about 209 mg·g^-1(based on carrier),and the highest enzyme activity of immobilized PGA(based on ImPGA) was 261 U·g^-1.Both the amount of loaded enzyme and the activity of ImPGA are at the same level of or higher than that in previous reports.After 10 consecutive operations,ImPGA still maintained 62% of its initial activity,indicating the good recovery property of ImPGA activity.The ionic liquid modified magnetic particles integrate the magnetic properties of Fe₃O₄ and the structure-tunable properties of ionic liquids,and have extensive potential uses in protein immobilization and magnetic bioseparation.This work may open up a novel strategy to immobilize proteins by ionic liquids.

关键词: magnetic iron oxide, magnetic nanoparticles, ionic liquids, penicillin G acylase, enzyme immobilization

CLC Number:

TQ925

ZHOU Huacong, LI Wei, SHOU Qinghui, GAO Hongshuai, XU Peng, DENG Fuli, LIU Huizhou. Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids[J]. Chin.J.Chem.Eng., 2012, 20(1): 146-151.

周华从, 李伟, 寿庆辉, 高红帅, 徐芃, 邓伏礼, 刘会洲. Immobilization of Penicillin G Acylase on Magnetic Nanoparticles Modified by Ionic Liquids[J]. Chinese Journal of Chemical Engineering, 2012, 20(1): 146-151.

References

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