Simultaneous Removal of Thiophene and Dibenzothiophene by Immobilized Pseudomonas delafieldii R-8 cells

›› 2012, Vol. 20 ›› Issue (1): 47-51.

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Simultaneous Removal of Thiophene and Dibenzothiophene by Immobilized Pseudomonas delafieldii R-8 cells

TANG Huang^1,2, LI Qiang¹, WANG Zelong^1,2, YAN Daojiang^1,2, XING Jianmin¹

1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sci-ences, Beijing 100190, China;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received:2011-05-09 Revised:2011-11-16 Online:2012-05-03 Published:2012-02-28
Supported by:
Supported by the National Natural Science Foundation of China (21076216,30970046)

Simultaneous Removal of Thiophene and Dibenzothiophene by Immobilized Pseudomonas delafieldii R-8 cells

唐煌^1,2, 李强¹, 王泽龙^1,2, 闫道江^1,2, 邢建民¹

1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sci-ences, Beijing 100190, China;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

通讯作者: XING Jianmin,E-mail:jmxing@home.ipe.ac.cn
基金资助:
Supported by the National Natural Science Foundation of China (21076216,30970046)

Abstract

Abstract: Biodesulfurization(BDS) is a promising technology for deep desulfurization.In this work,Pseudomonas delafieldii R-8 cells are immobilized in calcium alginate beads and used for BDS of transportation fuels.It is found that thiophene and dibenzothiophene(DBT) can be simultaneously metabolized by immobilized R-8 cells.The initial sulfur content in the model oil is 300 mg·kg^-1(thiophene:DBT 1:1).After 10 h of treatment,the thiophene concentration is reduced by 40%,while DBT is reduced by 25%.The utilization rate of thiophene is faster than that of DBT.Moreover,the oil/water ratio of alginate immobilized cells is studied to reduce the water volume in desulfurization systems.Long-term recycling of BDS by alginate immobilized cells is carried out with oil/water ratio at 5:1.The immobilized cells are successfully reused over 15 batch cycles.In the last batch,the desulfurization activity remains at least 75% of the first batch.

Key words: biodesulfurization, simultaneous removal, alginate immobilization, long-term recycling

摘要： Biodesulfurization(BDS) is a promising technology for deep desulfurization.In this work,Pseudomonas delafieldii R-8 cells are immobilized in calcium alginate beads and used for BDS of transportation fuels.It is found that thiophene and dibenzothiophene(DBT) can be simultaneously metabolized by immobilized R-8 cells.The initial sulfur content in the model oil is 300 mg·kg^-1(thiophene:DBT 1:1).After 10 h of treatment,the thiophene concentration is reduced by 40%,while DBT is reduced by 25%.The utilization rate of thiophene is faster than that of DBT.Moreover,the oil/water ratio of alginate immobilized cells is studied to reduce the water volume in desulfurization systems.Long-term recycling of BDS by alginate immobilized cells is carried out with oil/water ratio at 5:1.The immobilized cells are successfully reused over 15 batch cycles.In the last batch,the desulfurization activity remains at least 75% of the first batch.

关键词: biodesulfurization, simultaneous removal, alginate immobilization, long-term recycling

CLC Number:

TE624.1

TANG Huang, LI Qiang, WANG Zelong, YAN Daojiang, XING Jianmin. Simultaneous Removal of Thiophene and Dibenzothiophene by Immobilized Pseudomonas delafieldii R-8 cells[J]. , 2012, 20(1): 47-51.

唐煌, 李强, 王泽龙, 闫道江, 邢建民. Simultaneous Removal of Thiophene and Dibenzothiophene by Immobilized Pseudomonas delafieldii R-8 cells[J]. , 2012, 20(1): 47-51.

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Simultaneous Removal of Thiophene and Dibenzothiophene by Immobilized Pseudomonas delafieldii R-8 cells

Simultaneous Removal of Thiophene and Dibenzothiophene by Immobilized Pseudomonas delafieldii R-8 cells

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