Isolation and Characterization of Heterotrophic Nitrifying Strain W1*

Chin.J.Chem.Eng. ›› 2012, Vol. 20 ›› Issue (5): 995-1002.

• ENERGY, RESOURCES AND ENVIRONMENTAL TECHNOLOGY • Previous Articles Next Articles

Isolation and Characterization of Heterotrophic Nitrifying Strain W1^*

LÜ Yongkang¹, WANG Xun¹, LIU Bokai², LIU Yuxiang³, YANG Xiaohua¹

1. Key Laboratory of Coal Science and Technology, Taiyuan University of Technology, Taiyuan 030024, China;
2. Engineering & the Built Environment (EBE), University of Cape Town, Cape Town7701, South Africa;
3. College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China

Received:2011-06-20 Revised:2011-12-12 Online:2012-11-06 Published:2012-10-28
Supported by:
Supported by the National Natural Science Foundation of China (51078252);the International Cooperation Projects of Shanxi Province (2010081018);the Natural Science Foundation of Shanxi Province (2010011016-1)

Isolation and Characterization of Heterotrophic Nitrifying Strain W1^*

吕永康¹, 王荀¹, 刘博凯², 刘玉香³, 杨晓华¹

1. Key Laboratory of Coal Science and Technology, Taiyuan University of Technology, Taiyuan 030024, China;
2. Engineering & the Built Environment (EBE), University of Cape Town, Cape Town7701, South Africa;
3. College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China

通讯作者: LÜ Yongkang,E-mail:lykang@tyut.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China (51078252);the International Cooperation Projects of Shanxi Province (2010081018);the Natural Science Foundation of Shanxi Province (2010011016-1)

Abstract

Abstract: In a high concentration substrate medium, a heterotrophic bacterium with high removal efficiency of ammonium, named W1, was isolated from activated sludge of coking wastewater treatment facility. The bacterium was Gram-negative, rod-shaped, and identified preliminarily as Alcaligenes sp. according to its morphological and physiological properties and its 16S rRNA gene sequence analysis. In the high concentration ammonium medium (400 mg·L^-1 NH₄^--N), the effects of C source, N source, C/N ratio and initial pH of medium on ammonium removal were investigated in order to determine the optimal condition for strain W1. The maximum ammonium removal was around 95% in 4 days in an improved medium. The production of N₂ gas was examined in a closed system that was full of pure oxygen at the beginning. N₂ gas was detected in the system after 4 days of cultivation, which further testified that strain W1 has heterotrophic nitrification and aerobic denitrification abilities simultaneously.

Key words: heterotrophic nitrification, aerobic de-nitrification, high concentration ammonium, Alcaligenes sp.

摘要： In a high concentration substrate medium, a heterotrophic bacterium with high removal efficiency of ammonium, named W1, was isolated from activated sludge of coking wastewater treatment facility. The bacterium was Gram-negative, rod-shaped, and identified preliminarily as Alcaligenes sp. according to its morphological and physiological properties and its 16S rRNA gene sequence analysis. In the high concentration ammonium medium (400 mg·L^-1 NH₄^--N), the effects of C source, N source, C/N ratio and initial pH of medium on ammonium removal were investigated in order to determine the optimal condition for strain W1. The maximum ammonium removal was around 95% in 4 days in an improved medium. The production of N₂ gas was examined in a closed system that was full of pure oxygen at the beginning. N₂ gas was detected in the system after 4 days of cultivation, which further testified that strain W1 has heterotrophic nitrification and aerobic denitrification abilities simultaneously.

关键词: heterotrophic nitrification, aerobic de-nitrification, high concentration ammonium, Alcaligenes sp.

LÜ Yongkang, WANG Xun, LIU Bokai, LIU Yuxiang, YANG Xiaohua. Isolation and Characterization of Heterotrophic Nitrifying Strain W1^*[J]. Chin.J.Chem.Eng., 2012, 20(5): 995-1002.

吕永康, 王荀, 刘博凯, 刘玉香, 杨晓华. Isolation and Characterization of Heterotrophic Nitrifying Strain W1^*[J]. Chinese Journal of Chemical Engineering, 2012, 20(5): 995-1002.

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Isolation and Characterization of Heterotrophic Nitrifying Strain W1^*

Isolation and Characterization of Heterotrophic Nitrifying Strain W1^*

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