Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99-125 lipase via adsorption

doi:10.1016/j.cjche.2015.02.002

Abstract

Abstract: Magnetically modified palygorskite composites were synthesized with γ-Fe₂O₃ dispersing on the external surface of clay mineral. The magnetic clay was characterized with Fourier transform infrared, X-ray diffraction, transmission electron microscopy, and vibrating sample magnetometer. Candida sp. 99-125 lipase was immobilized on magnetic palygorskite composites by physical adsorption with enzyme loading of 41.5mg·g^-1 support and enzyme activity of 2631.6 U·(g support)^-1. The immobilized lipase exhibit better thermal and broader pH stability and excellent reusability compared with free lipase.

Key words: Magnetically modified palygorskite, Immobilized lipase, Adsorption, Stability, Reusability

摘要： Magnetically modified palygorskite composites were synthesized with γ-Fe₂O₃ dispersing on the external surface of clay mineral. The magnetic clay was characterized with Fourier transform infrared, X-ray diffraction, transmission electron microscopy, and vibrating sample magnetometer. Candida sp. 99-125 lipase was immobilized on magnetic palygorskite composites by physical adsorption with enzyme loading of 41.5mg·g^-1 support and enzyme activity of 2631.6 U·(g support)^-1. The immobilized lipase exhibit better thermal and broader pH stability and excellent reusability compared with free lipase.

关键词: Magnetically modified palygorskite, Immobilized lipase, Adsorption, Stability, Reusability

Ya Li, Jicheng Hu, Pingfang Han. Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99-125 lipase via adsorption[J]. Chin.J.Chem.Eng., 2015, 23(5): 822-826.

Ya Li, Jicheng Hu, Pingfang Han. Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99-125 lipase via adsorption[J]. Chinese Journal of Chemical Engineering, 2015, 23(5): 822-826.

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