Screening and pilot-scale evaluation of a highly efficient pesticide-degrading Pseudomonas sp. strain BL5

doi:10.1016/j.cjche.2024.07.006

Chinese Journal of Chemical Engineering ›› 2024, Vol. 74 ›› Issue (10): 287-294.DOI: 10.1016/j.cjche.2024.07.006

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Screening and pilot-scale evaluation of a highly efficient pesticide-degrading Pseudomonas sp. strain BL5

Rui Chen¹, Xinyu Zuo¹, He Bai², Ruolin Qin¹, Zhiguo Chen², Yiyun Liu², Wenqing Cao², Jingpeng Song², Xiaoqiang Jia^1,3

1 Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2 Tianjin Huakan Environmental Protection Technology Co., Ltd., Tianjin 300170, China;
3 Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China

Received:2024-03-19 Revised:2024-07-13 Accepted:2024-07-15 Online:2024-08-08 Published:2024-10-28
Contact: Xiaoqiang Jia,Tel.:+86 18920698855.E-mail:xqjia@tju.edu.cn
Supported by:
This research was supported by the National Key Research and Development Program of China (2018YFA0902100), the National Natural Science Foundation of China (22178262) and the Tianjin Key Research and Development Program (23YFZCSN00110).

Screening and pilot-scale evaluation of a highly efficient pesticide-degrading Pseudomonas sp. strain BL5

Rui Chen¹, Xinyu Zuo¹, He Bai², Ruolin Qin¹, Zhiguo Chen², Yiyun Liu², Wenqing Cao², Jingpeng Song², Xiaoqiang Jia^1,3

1 Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2 Tianjin Huakan Environmental Protection Technology Co., Ltd., Tianjin 300170, China;
3 Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China

通讯作者: Xiaoqiang Jia,Tel.:+86 18920698855.E-mail:xqjia@tju.edu.cn
基金资助:
This research was supported by the National Key Research and Development Program of China (2018YFA0902100), the National Natural Science Foundation of China (22178262) and the Tianjin Key Research and Development Program (23YFZCSN00110).

Abstract

Abstract: The widespread use of pesticides has caused serious harm to ecosystems, necessitating effective and environmentally friendly treatment methods. Bioremediation stands out as a promising approach for pollutant treatment, wherein the metabolic activities of microorganisms can transform toxic pesticides into compounds with lower or no toxicity. In this study, we obtained eight pesticide-degrading strains from pesticide-contaminated sites through continuous enrichment and screening. Four highly efficient pesticide-degrading strains (degradation ratios exceeding 80%) were identified. Among them, Pseudomonas sp. BL5 exhibited the strongest growth (exceeding 10⁹ CFU·ml^-1) and outstanding degradation of benzene derivatives and chlorinated hydrocarbons at both laboratory and pilot scales, with degradation ratios exceeding 98% and 99.6%, respectively. This research provides new tools and insights for the bioremediation of pesticide-related pollutants.

Key words: Pesticides, Pilot-scale, Degradation, Remediation, Pseudomonas sp., Benzene

摘要： The widespread use of pesticides has caused serious harm to ecosystems, necessitating effective and environmentally friendly treatment methods. Bioremediation stands out as a promising approach for pollutant treatment, wherein the metabolic activities of microorganisms can transform toxic pesticides into compounds with lower or no toxicity. In this study, we obtained eight pesticide-degrading strains from pesticide-contaminated sites through continuous enrichment and screening. Four highly efficient pesticide-degrading strains (degradation ratios exceeding 80%) were identified. Among them, Pseudomonas sp. BL5 exhibited the strongest growth (exceeding 10⁹ CFU·ml^-1) and outstanding degradation of benzene derivatives and chlorinated hydrocarbons at both laboratory and pilot scales, with degradation ratios exceeding 98% and 99.6%, respectively. This research provides new tools and insights for the bioremediation of pesticide-related pollutants.

关键词: Pesticides, Pilot-scale, Degradation, Remediation, Pseudomonas sp., Benzene

Rui Chen, Xinyu Zuo, He Bai, Ruolin Qin, Zhiguo Chen, Yiyun Liu, Wenqing Cao, Jingpeng Song, Xiaoqiang Jia. Screening and pilot-scale evaluation of a highly efficient pesticide-degrading Pseudomonas sp. strain BL5[J]. Chinese Journal of Chemical Engineering, 2024, 74(10): 287-294.

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Screening and pilot-scale evaluation of a highly efficient pesticide-degrading Pseudomonas sp. strain BL5

Screening and pilot-scale evaluation of a highly efficient pesticide-degrading Pseudomonas sp. strain BL5

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