Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing

doi:10.1016/S1004-9541(13)60510-8

Abstract

Abstract: method of extracting astaxanthin from Phaffia rhodozyma with various solvents after acid washing was investigated. The extraction efficiency was distinctly increased after acid washing of P. rhodozyma cells. When the concentration of HCl was 0.4 mol·L^-1, the highest extraction efficiency of astaxanthin was achieved which was about three times higher than the control. Acetone or benzene as single polar or non-polar solvent was the most effective solvent in our research. With a combination of isopropanol and n-hexane (volume ratio of 2:1), the maximal extraction efficiency was achieved, approximately 60% higher than that obtained with a single solvent. The liquid-solid ratio and the extracting time were also optimized. Under the optimum extraction conditions, the extraction yield of astaxanthin exceeded 98%.

Key words: astaxanthin, Phaffia rhodozyma, acid wash, extraction, solvent

摘要： method of extracting astaxanthin from Phaffia rhodozyma with various solvents after acid washing was investigated. The extraction efficiency was distinctly increased after acid washing of P. rhodozyma cells. When the concentration of HCl was 0.4 mol·L^-1, the highest extraction efficiency of astaxanthin was achieved which was about three times higher than the control. Acetone or benzene as single polar or non-polar solvent was the most effective solvent in our research. With a combination of isopropanol and n-hexane (volume ratio of 2:1), the maximal extraction efficiency was achieved, approximately 60% higher than that obtained with a single solvent. The liquid-solid ratio and the extracting time were also optimized. Under the optimum extraction conditions, the extraction yield of astaxanthin exceeded 98%.

关键词: astaxanthin, Phaffia rhodozyma, acid wash, extraction, solvent

YIN Chunhua, YANG Shuzhen, LIU Xiaolu, YAN Hai . Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing[J]. Chin.J.Chem.Eng., 2013, 21(7): 776-780.

尹春华, 杨淑珍, 刘晓璐, 闫海. Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing[J]. Chinese Journal of Chemical Engineering, 2013, 21(7): 776-780.

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