Lipase Catalyzed Synthesis of Medium-chain Biodiesel from Cinnamonum camphora Seed Oil

doi:10.1016/j.cjche.2014.09.011

›› 2014, Vol. 22 ›› Issue (11/12): 1215-1219.DOI: 10.1016/j.cjche.2014.09.011

• CATALYSIS, KINETICS AND REACTION ENGINEERING • Previous Articles Next Articles

Lipase Catalyzed Synthesis of Medium-chain Biodiesel from Cinnamonum camphora Seed Oil

Junfeng Liu¹, Li Deng^1,2, Meng Wang¹, Kaili Nie^1,2, Luo Liu¹, Tianwei Tan¹, Fang Wang^1,3

1 Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China;
2 Amoy-BUCT Industrial of Bio-technovation Institute, Amoy 361022, China;
3 State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China

Received:2013-01-05 Revised:2013-08-19 Online:2014-12-24 Published:2014-12-28
Supported by:
Supported by Key Projects in the National Science & Technology Pillar Programduring the 12th Five-year Plan Period (2011BAD22B04), the National Basic Research Program of China (973 program) (2011CB710800, 2011CB200905, 2009CB724703), the National Natural Science Foundation of China (21076017), the National High-Tech R&D Program of China (863 Program) (2012AA021404, 2012AA021402), the Fundamental Research Funds for the Central Universities (ZY1331).

Lipase Catalyzed Synthesis of Medium-chain Biodiesel from Cinnamonum camphora Seed Oil

Junfeng Liu¹, Li Deng^1,2, Meng Wang¹, Kaili Nie^1,2, Luo Liu¹, Tianwei Tan¹, Fang Wang^1,3

1 Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China;
2 Amoy-BUCT Industrial of Bio-technovation Institute, Amoy 361022, China;
3 State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China

通讯作者: Li Deng
基金资助:
Supported by Key Projects in the National Science & Technology Pillar Programduring the 12th Five-year Plan Period (2011BAD22B04), the National Basic Research Program of China (973 program) (2011CB710800, 2011CB200905, 2009CB724703), the National Natural Science Foundation of China (21076017), the National High-Tech R&D Program of China (863 Program) (2012AA021404, 2012AA021402), the Fundamental Research Funds for the Central Universities (ZY1331).

Abstract

Abstract: The non-edible camphor tree seed oil was extracted and catalyzed by immobilized lipase for biodiesel production. The oil yield from camphor tree seeds reached 35.2% of seed weight by twice microwave-assisted extractions. Gas chromatography showed that free fatty acid content in camphor tree seed oil was 1.88%, and the main fatty acids were capric acid (53.4%) and lauric acid (38.7%).With immobilized lipase Candida sp. 99-125 as catalyst, several important factors for reaction conditions were examined through orthogonal experiments. The optimum conditions were obtained: water content and enzyme loading were both 15% with a molar ratio of 1:3.5 (oil/ethanol), and the process of alcoholysis was in nine steps at 40 ℃ for 24 h, with agitation at 170 r·min^-1. As a result, the medium-chain biodiesel yield was 93.5%. The immobilized lipase was stable when it was used repeatedly for 210 h.

Key words: Camphor tree seed oil, Medium-chain biodiesel, Lipase, Ethanolysis

摘要： The non-edible camphor tree seed oil was extracted and catalyzed by immobilized lipase for biodiesel production. The oil yield from camphor tree seeds reached 35.2% of seed weight by twice microwave-assisted extractions. Gas chromatography showed that free fatty acid content in camphor tree seed oil was 1.88%, and the main fatty acids were capric acid (53.4%) and lauric acid (38.7%).With immobilized lipase Candida sp. 99-125 as catalyst, several important factors for reaction conditions were examined through orthogonal experiments. The optimum conditions were obtained: water content and enzyme loading were both 15% with a molar ratio of 1:3.5 (oil/ethanol), and the process of alcoholysis was in nine steps at 40 ℃ for 24 h, with agitation at 170 r·min^-1. As a result, the medium-chain biodiesel yield was 93.5%. The immobilized lipase was stable when it was used repeatedly for 210 h.

关键词: Camphor tree seed oil, Medium-chain biodiesel, Lipase, Ethanolysis

Junfeng Liu, Li Deng, Meng Wang, Kaili Nie, Luo Liu, Tianwei Tan, Fang Wang. Lipase Catalyzed Synthesis of Medium-chain Biodiesel from Cinnamonum camphora Seed Oil[J]. , 2014, 22(11/12): 1215-1219.

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Lipase Catalyzed Synthesis of Medium-chain Biodiesel from Cinnamonum camphora Seed Oil

Lipase Catalyzed Synthesis of Medium-chain Biodiesel from Cinnamonum camphora Seed Oil

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