SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2020, Vol. 28 ›› Issue (1): 242-248.DOI: 10.1016/j.cjche.2019.03.002

• Biotechnology and Bioengineering • Previous Articles     Next Articles

Zwitterionic polymer-coated porous poly(vinyl acetate-divinyl benzene) microsphere: A new support for enhanced performance of immobilized lipase

Yifeng Li, Chunyu Zhang, Yan Sun   

  1. Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering(Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300354, China
  • Received:2019-01-11 Revised:2019-03-05 Online:2020-03-31 Published:2020-01-28
  • Contact: Yan Sun
  • Supported by:
    Supported by the National Natural Science Foundation of China (21621004, 21878222).

Zwitterionic polymer-coated porous poly(vinyl acetate-divinyl benzene) microsphere: A new support for enhanced performance of immobilized lipase

Yifeng Li, Chunyu Zhang, Yan Sun   

  1. Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering(Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300354, China
  • 通讯作者: Yan Sun
  • 基金资助:
    Supported by the National Natural Science Foundation of China (21621004, 21878222).

Abstract: Enzyme immobilization has attracted great attention for improving the performance of enzymes in industrial applications. This work was designed to create a new support for Candida rugosa lipase (CRL) immobilization. A porous poly(vinyl acetate-divinyl benzene) microsphere coated by a zwitterionic polymer, poly(maleic anhydride-alt-1-octadecene) and N,N-dimethylethylenediamine derivative, was developed for CRL immobilization via hydrophobic binding. The catalytic activity, reaction kinetics, stabilities and reusability of the immobilized CRL were investigated. It demonstrated the success of the zwitterionic polymer coating and subsequent CRL immobilization on the porous microsphere. The immobilized lipase (p2-MS-CRL) reached 27.6 mg·g-1 dry carrier and displayed a specific activity 1.5 times higher than free CRL. The increase of Vmax and decrease of Km were also observed, indicating the improvement of catalytic activity and enzyme-substrate affinity of the immobilized lipase. Besides, p2-MS-CRL exhibited significantly enhanced thermal stability and pH tolerance. The improved performance was considered due to the interfacial activation regulated by the hydrophobic interaction and stabilization effect arisen by the zwitterionic polymer coating. This study has thus proved the advantages of the zwitterionic polymer-coated porous carrier for lipase immobilization and its potential for further development in various enzyme immobilizations.

Key words: Biocatalysis, Lipase immobilization, Zwitterionic polymer, Hydrophobic binding, Interfacial activation, Stabilization

摘要: Enzyme immobilization has attracted great attention for improving the performance of enzymes in industrial applications. This work was designed to create a new support for Candida rugosa lipase (CRL) immobilization. A porous poly(vinyl acetate-divinyl benzene) microsphere coated by a zwitterionic polymer, poly(maleic anhydride-alt-1-octadecene) and N,N-dimethylethylenediamine derivative, was developed for CRL immobilization via hydrophobic binding. The catalytic activity, reaction kinetics, stabilities and reusability of the immobilized CRL were investigated. It demonstrated the success of the zwitterionic polymer coating and subsequent CRL immobilization on the porous microsphere. The immobilized lipase (p2-MS-CRL) reached 27.6 mg·g-1 dry carrier and displayed a specific activity 1.5 times higher than free CRL. The increase of Vmax and decrease of Km were also observed, indicating the improvement of catalytic activity and enzyme-substrate affinity of the immobilized lipase. Besides, p2-MS-CRL exhibited significantly enhanced thermal stability and pH tolerance. The improved performance was considered due to the interfacial activation regulated by the hydrophobic interaction and stabilization effect arisen by the zwitterionic polymer coating. This study has thus proved the advantages of the zwitterionic polymer-coated porous carrier for lipase immobilization and its potential for further development in various enzyme immobilizations.

关键词: Biocatalysis, Lipase immobilization, Zwitterionic polymer, Hydrophobic binding, Interfacial activation, Stabilization