Cell-free synthetic biology in the new era of enzyme engineering

doi:10.1016/j.cjche.2020.05.037

Abstract

Abstract: With the gradual rise of enzyme engineering, it has played an essential role in synthetic biology, medicine, and biomanufacturing. However, due to the limitation of the cell membrane, the complexity of cellular metabolism, the difficulty of controlling the reaction environment, and the toxicity of some metabolic products in traditional in vivo enzyme engineering, it is usually problematic to express functional enzymes and produce a high yield of synthesized compounds. Recently, cell-free synthetic biology methods for enzyme engineering have been proposed as alternative strategies. This cell-free method has no limitation of the cell membrane and no need to maintain cell viability, and each biosynthetic pathway is highly flexible. This property makes cell-free approaches suitable for the production of valuable products such as functional enzymes and chemicals that are difficult to synthesize. This article aims to discuss the latest advances in cell-free enzyme engineering, assess the trend of this developing topical filed, and analyze its prospects.

Key words: Cell-free synthetic biology, Enzyme engineering, Metabolic engineering

摘要： With the gradual rise of enzyme engineering, it has played an essential role in synthetic biology, medicine, and biomanufacturing. However, due to the limitation of the cell membrane, the complexity of cellular metabolism, the difficulty of controlling the reaction environment, and the toxicity of some metabolic products in traditional in vivo enzyme engineering, it is usually problematic to express functional enzymes and produce a high yield of synthesized compounds. Recently, cell-free synthetic biology methods for enzyme engineering have been proposed as alternative strategies. This cell-free method has no limitation of the cell membrane and no need to maintain cell viability, and each biosynthetic pathway is highly flexible. This property makes cell-free approaches suitable for the production of valuable products such as functional enzymes and chemicals that are difficult to synthesize. This article aims to discuss the latest advances in cell-free enzyme engineering, assess the trend of this developing topical filed, and analyze its prospects.

关键词: Cell-free synthetic biology, Enzyme engineering, Metabolic engineering

Nan Jiang, Lianju Ma, Yuan Lu. Cell-free synthetic biology in the new era of enzyme engineering[J]. Chinese Journal of Chemical Engineering, 2020, 28(11): 2810-2816.

Nan Jiang, Lianju Ma, Yuan Lu. Cell-free synthetic biology in the new era of enzyme engineering[J]. 中国化学工程学报, 2020, 28(11): 2810-2816.

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