SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2021, Vol. 38 ›› Issue (10): 237-246.DOI: 10.1016/j.cjche.2021.04.019

• Biotechnology and Bioengineering • Previous Articles     Next Articles

Nickel-Carnosine complex: A new carrier for enzymes immobilization by affinity adsorption

Junyang Xu1, Yanjun Jiang1,3, Liya Zhou1, Li Ma1, Zhihong Huang1, Jiafu Shi2, Jing Gao1, Ying He1   

  1. 1. School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China;
    2. Tianjin Key Lab of Biomass/Wastes Utilization, School of Environmental Science & Engineering, Tianjin University, Tianjin 300072, China;
    3. National-Local Joint Engineering Laboratory for Energy Conservation of Chemical Process Integration and Resources Utilization, Hebei University of Technology, Tianjin 300130, China
  • Received:2020-12-31 Revised:2021-04-06 Online:2021-12-02 Published:2021-10-28
  • Contact: Liya Zhou, Ying He

Nickel-Carnosine complex: A new carrier for enzymes immobilization by affinity adsorption

Junyang Xu1, Yanjun Jiang1,3, Liya Zhou1, Li Ma1, Zhihong Huang1, Jiafu Shi2, Jing Gao1, Ying He1   

  1. 1. School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China;
    2. Tianjin Key Lab of Biomass/Wastes Utilization, School of Environmental Science & Engineering, Tianjin University, Tianjin 300072, China;
    3. National-Local Joint Engineering Laboratory for Energy Conservation of Chemical Process Integration and Resources Utilization, Hebei University of Technology, Tianjin 300130, China
  • 通讯作者: Liya Zhou, Ying He

Abstract: Immobilization is an effective method to promote the application of enzyme industry for improving the stability and realizing recovery of enzyme. To some extent, the performance of immobilized enzyme depends on the choice of carrier material. Therefore, the development of new carrier materials has been one of the key issues concerned by enzyme immobilization researchers. In this work, a novel organic-inorganic hybrid material, nickel-carnosine complex (NiCar), was synthesized for the first time by solvothermal method. The obtained NiCar exhibits spherical morphology, hierarchical porosity and abundant unsaturated coordination nickel ions, which provide excellent anchoring sites for the immobilization of proteins. His-tagged organophosphate-degrading enzyme (OpdA) and ω-transaminase (ω-TA) were used as model enzymes to evaluate the performance of NiCar as a carrier. By a simple adsorption process, the enzyme molecules can be fixed on the particles of NiCar, and the stability and reusability are significantly improved. The analysis of protein adsorption on NiCar verified that the affinity adsorption between the imidazole functional group on the protein and the unsaturated coordination nickel ions on NiCar was the main force in the immobilization process, which provided an idea way for the development of new enzyme immobilization carriers.

Key words: Nickel carnosine complex, Organic-inorganic hybrid materials, Carrier, Enzymes, Immobilization

摘要: Immobilization is an effective method to promote the application of enzyme industry for improving the stability and realizing recovery of enzyme. To some extent, the performance of immobilized enzyme depends on the choice of carrier material. Therefore, the development of new carrier materials has been one of the key issues concerned by enzyme immobilization researchers. In this work, a novel organic-inorganic hybrid material, nickel-carnosine complex (NiCar), was synthesized for the first time by solvothermal method. The obtained NiCar exhibits spherical morphology, hierarchical porosity and abundant unsaturated coordination nickel ions, which provide excellent anchoring sites for the immobilization of proteins. His-tagged organophosphate-degrading enzyme (OpdA) and ω-transaminase (ω-TA) were used as model enzymes to evaluate the performance of NiCar as a carrier. By a simple adsorption process, the enzyme molecules can be fixed on the particles of NiCar, and the stability and reusability are significantly improved. The analysis of protein adsorption on NiCar verified that the affinity adsorption between the imidazole functional group on the protein and the unsaturated coordination nickel ions on NiCar was the main force in the immobilization process, which provided an idea way for the development of new enzyme immobilization carriers.

关键词: Nickel carnosine complex, Organic-inorganic hybrid materials, Carrier, Enzymes, Immobilization