SCI和EI收录∣中国化工学会会刊

›› 2010, Vol. 18 ›› Issue (4): 681-686.

• BIOTECHNOLOGY AND BIOENGINEERING • Previous Articles     Next Articles

Influence of L-Cysteine Concentration on Oxidation-reduction Potential and Biohydrogen Production

CHEN Huoqing1,2, MA Xiaoxuan1,2, FAN Daidi1,2, LUO Yan'e1,2, GAO Pengfei1,2, YANG Chanyuan1,2   

  1. 1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China;
    2. Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical and Engineering, Northwest University, Xi'an 710069, China
  • Received:2009-11-20 Revised:2010-05-20 Online:2010-08-28 Published:2010-08-28

Influence of L-Cysteine Concentration on Oxidation-reduction Potential and Biohydrogen Production

陈火晴1,2, 马晓轩1,2, 范代娣1,2, 骆艳娥1,2, 高鹏飞1,2, 杨婵媛1,2   

  1. 1. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China;
    2. Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical and Engineering, Northwest University, Xi'an 710069, China
  • 通讯作者: FAN Daidi, E-mail:fandaidi@nwu.edu.cn
  • 基金资助:
    Supported by the National High Technology Research and Development Program of China(2006AA02Z246,2007AA03Z456);the National Natural Science Foundation of China(20776119);the Specialized Research Fund for the Doctoral Program of Higher Education of China(20096101120023);Shaanxi Provincial Natural Science Foundation(SJ08B03);Shaanxi Key Subject Program,China

Abstract: The effects of L-cysteine concentration on biohydrogen production by Enterobacterium Bacterium M580 were investigated in batch cultivation.The experimental results showed that L-cysteine could enhance the cell growth,hydrogen production rate and hydrogen yield when its concentration was less than 500 mg·L-1,while it had negative effects when its concentration was higher than 500 mg·L-1.The hydrogen production was the highest 1.29 mol·mol-1(H2/glucose) when 300 mg·L-1 L-cysteine was added into the culture,and the yield was 9.4% higher than that in the control.The oxidation-reduction potential(ORP),which was influenced by L-cysteine,also affected hydrogen production.The ORP values were in the range -300 mV to -150 mV when the L-cysteine concentration was higher than 500 mg·L-1.Although the ORP in this range was favorable for hydrogen production,it was not suitable for the biomass growth.Hence,less hydrogen was produced.When the L-cysteine concentration was lower than 500 mg·L-1,the ORP was more suitable for both biomass growth and hydrogen production.In addition,at least 91%glucose was consumed when L-cysteine was added to the culture media,compared to the 97.37% consumption without L-cysteine added.

Key words: anaerobic fermentation, biohydrogen, L-cysteine, ORP

摘要: The effects of L-cysteine concentration on biohydrogen production by Enterobacterium Bacterium M580 were investigated in batch cultivation.The experimental results showed that L-cysteine could enhance the cell growth,hydrogen production rate and hydrogen yield when its concentration was less than 500 mg·L-1,while it had negative effects when its concentration was higher than 500 mg·L-1.The hydrogen production was the highest 1.29 mol·mol-1(H2/glucose) when 300 mg·L-1 L-cysteine was added into the culture,and the yield was 9.4% higher than that in the control.The oxidation-reduction potential(ORP),which was influenced by L-cysteine,also affected hydrogen production.The ORP values were in the range -300 mV to -150 mV when the L-cysteine concentration was higher than 500 mg·L-1.Although the ORP in this range was favorable for hydrogen production,it was not suitable for the biomass growth.Hence,less hydrogen was produced.When the L-cysteine concentration was lower than 500 mg·L-1,the ORP was more suitable for both biomass growth and hydrogen production.In addition,at least 91%glucose was consumed when L-cysteine was added to the culture media,compared to the 97.37% consumption without L-cysteine added.

关键词: anaerobic fermentation, biohydrogen, L-cysteine, ORP