Analysis of Metabolic Products by Response Surface Methodology for Production of Human-like Collagen II

›› 2010, Vol. 18 ›› Issue (5): 830-836.

• BIOTECHNOLOGY AND BIOENGINEERING • Previous Articles Next Articles

Analysis of Metabolic Products by Response Surface Methodology for Production of Human-like Collagen II

GUO Jiaqing, LUO Yan'e, FAN Daidi, GAO Pengfei, MA Xiaoxuan, ZHU Chenhui

1. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China;
2. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China

Received:2010-02-24 Revised:2010-07-05 Online:2010-10-28 Published:2010-10-28
Supported by:
Supported by the National High Technology Research and Development Program of China (2006AA02Z246, 2007AA03Z456);the National Natural Science Foundation of China (20776119, 21076169);Xi'an Research and Development Program(CX0735);the Scientific Research Program of Shaanxi Provincial Department of Education , China (07JK417, 07JC16, JG08181);the Natural Science Foundation of Shaanxi Province (2010JQ2012);the Specialized Research Fund for the Doctoral Program of Higher Education of China (20096101120023, 20096101110014) ;Shaanxi Key Subject Program, China

Analysis of Metabolic Products by Response Surface Methodology for Production of Human-like Collagen II

郭佳庆, 骆艳娥, 范代娣, 高鹏飞, 马晓轩, 朱晨辉

1. Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, China;
2. Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, China

通讯作者: LUO Yan'e,E-mail:fandaidi@nwu.edu.cn;luoyane2004@126.com
基金资助:
Supported by the National High Technology Research and Development Program of China (2006AA02Z246, 2007AA03Z456);the National Natural Science Foundation of China (20776119, 21076169);Xi'an Research and Development Program(CX0735);the Scientific Research Program of Shaanxi Provincial Department of Education , China (07JK417, 07JC16, JG08181);the Natural Science Foundation of Shaanxi Province (2010JQ2012);the Specialized Research Fund for the Doctoral Program of Higher Education of China (20096101120023, 20096101110014) ;Shaanxi Key Subject Program, China

Abstract

Abstract: Recombinant Escherichia coli BL21 is used to produce human-like collagen. The key constituents of media are optimized using response surface methodology (RSM). Before thermal induction, the highest biomass production and the lowest production of some hazardous by-products, especially acetic acid, were obtained in the media containing 0.085 mol·L^-1 glucose and 0.019 mol·L^-1 nitrogen (carbon-nitrogen ratio, 4.47:1). After thermal induction, when the concentrations of glucose and nitrogen in the media were 0.065 mol·L^-1 and 0.017 mol·L^-1, respectively (carbon-nitrogen ratio, 3.82:1), the productivity of human-like collagen per cell was the highest while that of acetic acid was the lowest. The extended analysis showed that the production of lactic acid and propionic acid increased while that of some intermediate acids of the tricarboxylic acid cycle decreased if the dose of glucose increased.

Key words: carbon-nitrogen ratio, human-like collagen, organic acid metabolism, recombinant Escherichia coli, response surface methodology

摘要： Recombinant Escherichia coli BL21 is used to produce human-like collagen. The key constituents of media are optimized using response surface methodology (RSM). Before thermal induction, the highest biomass production and the lowest production of some hazardous by-products, especially acetic acid, were obtained in the media containing 0.085 mol·L^-1 glucose and 0.019 mol·L^-1 nitrogen (carbon-nitrogen ratio, 4.47:1). After thermal induction, when the concentrations of glucose and nitrogen in the media were 0.065 mol·L^-1 and 0.017 mol·L^-1, respectively (carbon-nitrogen ratio, 3.82:1), the productivity of human-like collagen per cell was the highest while that of acetic acid was the lowest. The extended analysis showed that the production of lactic acid and propionic acid increased while that of some intermediate acids of the tricarboxylic acid cycle decreased if the dose of glucose increased.

关键词: carbon-nitrogen ratio, human-like collagen, organic acid metabolism, recombinant Escherichia coli, response surface methodology

GUO Jiaqing, LUO Yan'e, FAN Daidi, GAO Pengfei, MA Xiaoxuan, ZHU Chenhui. Analysis of Metabolic Products by Response Surface Methodology for Production of Human-like Collagen II[J]. , 2010, 18(5): 830-836.

郭佳庆, 骆艳娥, 范代娣, 高鹏飞, 马晓轩, 朱晨辉. Analysis of Metabolic Products by Response Surface Methodology for Production of Human-like Collagen II[J]. , 2010, 18(5): 830-836.

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Analysis of Metabolic Products by Response Surface Methodology for Production of Human-like Collagen II

Analysis of Metabolic Products by Response Surface Methodology for Production of Human-like Collagen II

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