Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125

›› 2011, Vol. 19 ›› Issue (6): 978-982.

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Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125

DENG Li¹, WANG Xiaojing¹, NIE Kaili¹, WANG Fang¹, LIU Junfeng¹, WANG Pu², TAN Tianwei¹

1. College of Life Science and Technology, Beijing Key Laboratory of Bioprocess, Biorefinery Research and Engineering Center, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, China;
2. College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China

Received:2011-05-10 Revised:2011-09-29 Online:2012-04-24 Published:2011-12-28
Supported by:
Supported by the National High Technology Research and Development Program of China (2009AA02Z207);the National Natural Science Foundation of China (20876012);the Hi-Tech Research and Development Program of China (2009AA03Z232,2010AA101601)

Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125

邓利¹, 王晓静¹, 聂开立¹, 王芳¹, 刘军峰¹, 王璞², 谭天伟¹

1. College of Life Science and Technology, Beijing Key Laboratory of Bioprocess, Biorefinery Research and Engineering Center, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, China;
2. College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, China

通讯作者: LIU Junfeng, E-mail: dengli@mail.buct.edu.cn
基金资助:
Supported by the National High Technology Research and Development Program of China (2009AA02Z207);the National Natural Science Foundation of China (20876012);the Hi-Tech Research and Development Program of China (2009AA03Z232,2010AA101601)

Abstract

Abstract: Wax esters were synthesized in a solvent free system catalyzed by immobilized lipase from Candida sp. 99-125, with oleic acid and cetyl alcohol. The effects of substrate molar ratio, lipase dosage and water removal were investigated in a 50 ml flask incubated in a thermostatic cultivation cabinet. The optimized conditions were: temperature 40℃, shaking at 170 r·min^-1, acid/alcohol molar ratio 1:0.9, lipase dosage in 10% (by mass) of oleic acid, and open reaction for water removal. As a result, the conversion rate reached 98% for reaction of 8 h. The volume of reactor was scaled up to 1 L three-neck flask. The optimized parameters were: 200 r·min^-1 agitation speed, 2.5% (by mass) lipase dosage, others were the same as the parameters described above. The conversion rate reached 95% for reaction of 24 h. The lipase retained 46% conversion rate after reuse for 6, 7 batches. The products were purified by removing remained cetyl alcohol and fatty acids with ethanol and saturated sodium carbonate solution, respectively. The purity of the wax ester, cetyl oleate, was 96%. The physical and chemical properties of cetyl oleate were tested and compared with those of jojoba oil. The results show that the product cetyl oleate has great potential to use as the substitute of natural jojoba oil.

Key words: solvent free system, Candida sp. 99-125, lipase, wax ester

摘要： Wax esters were synthesized in a solvent free system catalyzed by immobilized lipase from Candida sp. 99-125, with oleic acid and cetyl alcohol. The effects of substrate molar ratio, lipase dosage and water removal were investigated in a 50 ml flask incubated in a thermostatic cultivation cabinet. The optimized conditions were: temperature 40℃, shaking at 170 r·min^-1, acid/alcohol molar ratio 1:0.9, lipase dosage in 10% (by mass) of oleic acid, and open reaction for water removal. As a result, the conversion rate reached 98% for reaction of 8 h. The volume of reactor was scaled up to 1 L three-neck flask. The optimized parameters were: 200 r·min^-1 agitation speed, 2.5% (by mass) lipase dosage, others were the same as the parameters described above. The conversion rate reached 95% for reaction of 24 h. The lipase retained 46% conversion rate after reuse for 6, 7 batches. The products were purified by removing remained cetyl alcohol and fatty acids with ethanol and saturated sodium carbonate solution, respectively. The purity of the wax ester, cetyl oleate, was 96%. The physical and chemical properties of cetyl oleate were tested and compared with those of jojoba oil. The results show that the product cetyl oleate has great potential to use as the substitute of natural jojoba oil.

关键词: solvent free system, Candida sp. 99-125, lipase, wax ester

DENG Li, WANG Xiaojing, NIE Kaili, WANG Fang, LIU Junfeng, WANG Pu, TAN Tianwei. Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125[J]. , 2011, 19(6): 978-982.

邓利, 王晓静, 聂开立, 王芳, 刘军峰, 王璞, 谭天伟. Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125[J]. , 2011, 19(6): 978-982.

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Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125

Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125

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