Chin.J.Chem.Eng. ›› 2018, Vol. 26 ›› Issue (12): 2601-2606.doi: 10.1016/j.cjche.2018.02.007

• Biotechnology and Bioengineering • Previous Articles     Next Articles

A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis

Dongsheng Xue1, Xuhao Zeng1, Chunjie Gong1, Dongqiang Lin2, Shanjing Yao2   

  1. 1 Key Laboratory of Fermentation Engineering(Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China;
    2 Department of Chemical and Bioengineering, Zhejiang University, Hangzhou 310027, China
  • Received:2017-11-11 Revised:2018-01-31 Online:2018-12-28 Published:2019-01-09
  • Contact: Shanjing Yao
  • Supported by:

    Supported by the Science and Technology Research Project of Hubei Provicial Department of Education(B2017041).

Abstract: The catalytic properties and thermodynamic kinetics of the endoglucanase from a marine Bacillus subtilis were analyzed. Optimum pH and temperature of the endoglucanase activity were 5.0 and 35℃. The endoglucanase activity, melt point temperature was 1.13 folds (247.02 U·ml-1), 2.1℃ higher (39.2℃) in 6% ethanol solution than that (218.60 U·ml-1), (37.1℃) in free ethanol. At 40℃-55℃, Gibbs free energy, ΔG, and the content of α-helix was higher in 6% ethanol solution than that in ethanol free solution. The increasing of α-helix content led to higher activity and better thermostability in ethanol solution. The cold adapt ethanol tolerant endoglucanase was valuable for bioethanol product by simultaneous saccharification and fermentation process.

Key words: Endoglucanase, Ethanol tolerant, Cold adapt, Bioethanol