A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis

doi:10.1016/j.cjche.2018.02.007

Abstract

Abstract: The catalytic properties and thermodynamic kinetics of the endoglucanase from a marine Bacillus subtilis were analyzed. Optimum pH and temperature of the endoglucanase activity were 5.0 and 35℃. The endoglucanase activity, melt point temperature was 1.13 folds (247.02 U·ml^-1), 2.1℃ higher (39.2℃) in 6% ethanol solution than that (218.60 U·ml^-1), (37.1℃) in free ethanol. At 40℃-55℃, Gibbs free energy, ΔG, and the content of α-helix was higher in 6% ethanol solution than that in ethanol free solution. The increasing of α-helix content led to higher activity and better thermostability in ethanol solution. The cold adapt ethanol tolerant endoglucanase was valuable for bioethanol product by simultaneous saccharification and fermentation process.

Key words: Endoglucanase, Ethanol tolerant, Cold adapt, Bioethanol

摘要： The catalytic properties and thermodynamic kinetics of the endoglucanase from a marine Bacillus subtilis were analyzed. Optimum pH and temperature of the endoglucanase activity were 5.0 and 35℃. The endoglucanase activity, melt point temperature was 1.13 folds (247.02 U·ml^-1), 2.1℃ higher (39.2℃) in 6% ethanol solution than that (218.60 U·ml^-1), (37.1℃) in free ethanol. At 40℃-55℃, Gibbs free energy, ΔG, and the content of α-helix was higher in 6% ethanol solution than that in ethanol free solution. The increasing of α-helix content led to higher activity and better thermostability in ethanol solution. The cold adapt ethanol tolerant endoglucanase was valuable for bioethanol product by simultaneous saccharification and fermentation process.

关键词: Endoglucanase, Ethanol tolerant, Cold adapt, Bioethanol

Dongsheng Xue, Xuhao Zeng, Chunjie Gong, Dongqiang Lin, Shanjing Yao. A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis[J]. Chin.J.Chem.Eng., 2018, 26(12): 2601-2606.

Dongsheng Xue, Xuhao Zeng, Chunjie Gong, Dongqiang Lin, Shanjing Yao. A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis[J]. Chinese Journal of Chemical Engineering, 2018, 26(12): 2601-2606.

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