Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii

doi:10.1016/j.cjche.2018.06.019

Chinese Journal of Chemical Engineering ›› 2019, Vol. 27 ›› Issue (5): 1166-1170.DOI: 10.1016/j.cjche.2018.06.019

• Biotechnology and Bioengineering • Previous Articles Next Articles

Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii

Dongsheng Xue¹, Xuhao Zeng¹, Dongqiang Lin², Shanjing Yao²

1 Key Laboratory of Fermentation Engineering(Hubei Universty of Technology), Ministry of Education, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China;
2 Department of Chemical and Bioengineering, Zhejiang University, Hangzhou 310027, China

Received:2018-03-30 Revised:2018-05-19 Online:2019-06-27 Published:2019-05-28
Contact: Shanjing Yao
Supported by:
Supported by the Science and Technology Research Project of Hubei Provincial Department of Education (B2017041).

Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii

Dongsheng Xue¹, Xuhao Zeng¹, Dongqiang Lin², Shanjing Yao²

1 Key Laboratory of Fermentation Engineering(Hubei Universty of Technology), Ministry of Education, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China;
2 Department of Chemical and Bioengineering, Zhejiang University, Hangzhou 310027, China

通讯作者: Shanjing Yao
基金资助:
Supported by the Science and Technology Research Project of Hubei Provincial Department of Education (B2017041).

Abstract

Abstract: A xylanase-producing bacterium, isolated from deep sea sediments, was identified as the cold-adapted marine species Acinetobacter Johnsonii. A cold-adapted marine species Acinetobacter Johnsonii could grow at 4℃. The optimum temperature and pH of xylanase from a cold-adapted marine species Acinetobacter Johnsonii were 55℃ and pH 6.0. Xylanase from a cold-adapted marine species Acinetobacter Johnsonii remained at 80% activity after incubation for 1 h at 65℃. The xylanase activity was 1.2-fold higher in 4% ethanol solution than in ethanol free solution. Gibbs free energy of denaturation, ΔG, was higher in 4% ethanol solution than in ethanol free solution. Thermostable ethanol tolerant xylanase was valuable for bioethanol production by simultaneous saccharification and fermentation process with xylan as a carbon source.

Key words: Xylanase, Ethanol tolerant, Thermostable, Cold-adapted, Acinetobacter Johnsonii

摘要： A xylanase-producing bacterium, isolated from deep sea sediments, was identified as the cold-adapted marine species Acinetobacter Johnsonii. A cold-adapted marine species Acinetobacter Johnsonii could grow at 4℃. The optimum temperature and pH of xylanase from a cold-adapted marine species Acinetobacter Johnsonii were 55℃ and pH 6.0. Xylanase from a cold-adapted marine species Acinetobacter Johnsonii remained at 80% activity after incubation for 1 h at 65℃. The xylanase activity was 1.2-fold higher in 4% ethanol solution than in ethanol free solution. Gibbs free energy of denaturation, ΔG, was higher in 4% ethanol solution than in ethanol free solution. Thermostable ethanol tolerant xylanase was valuable for bioethanol production by simultaneous saccharification and fermentation process with xylan as a carbon source.

关键词: Xylanase, Ethanol tolerant, Thermostable, Cold-adapted, Acinetobacter Johnsonii

Dongsheng Xue, Xuhao Zeng, Dongqiang Lin, Shanjing Yao. Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii[J]. Chinese Journal of Chemical Engineering, 2019, 27(5): 1166-1170.

Dongsheng Xue, Xuhao Zeng, Dongqiang Lin, Shanjing Yao. Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii[J]. 中国化学工程学报, 2019, 27(5): 1166-1170.

References

[1] A. Ebringerová, T. Heinze, Xylan and xylan derivatives-Biopolymers with valuable properties. 1. Naturally occurring xylans:Structures, isolation, procedure and properties, Macromol. Rapid Commun. 21(2000) 542-556.
[2] H.V. Scheller, P. Ulvskov, Hemicelluloses, Annu. Rev. Plant Biol. 61(2010) 263-289.
[3] A. Ebringerová, Z. Hromádková, T. Heinze, Hemicellulose, Adv. Polym. Sci. 186(2005) 1-67.
[4] V. Smil, Crop residues:Agriculture's Largest Harvest:Crop residues incorporate more than half of the world's agricultural phytomass, Bioscience 49(1999) 299-308.
[5] T. Collins, C. Gerday, G. Feller, Xylanases, xylanase families and extremophilic xylanases, FEMS Microbiol. Rev. 29(2005) 3-23.
[6] V. Juturu, J.C. Wu, Microbial xylanases:Engineering, production and industrial applications, Biotechnol. Adv. 30(2012) 1219-1227.
[7] G. Hilpmann, N. Becher, F.A. Pahner, B. Kusema, P. Mäki-Arvela, R. Lange, D.Yu. Murzin, T. Salmi, Acid hydrolysis of xylan, Catal. Today 259(2016) 376-380.
[8] J. Kallmeyer, R. Pockalny, R.R. Adhikari, D.C. Smith, S. D'Hondt, Global distribution of microbial abundance and biomass in subseafloor sediment, Proc. Natl. Acad. Sci. 109(2012) 16213-16216.
[9] K. Horikoshi, Barophiles:Deep-sea microorganisms adapted to an extreme environment, Curr. Opin. Microbiol. 1(1998) 291-295.
[10] I.P.G. Marshall, S.M. Karst, P.H. Nielsen, B.B. Jørgensen, Metagenomes from deep Baltic Sea sediments reveal how past and presentenvironmental conditions determine microbial community composition, Mar. Genomics 37(2018) 58-68.
[11] B.B. Jørgensen, I.P.G. Marshall, Slow microbial life in the seabed, Annu. Rev. Mar. Sci. 8(2016) 1-22.
[12] P.A. Skovgaard, H. Jørgensen, Influence of high temperature and ethanol on thermostable lignocellulolytic enzymes, J. Ind. Microbiol. Biotechnol. 40(2013) 447-456.
[13] P.C.Y. Woo, S.K.P. Lau, J.L.L. Teng, H. Tse, K.Y. Yuen, Then and now:use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories, Clin. Microbiol. Infect. 14(2008) 908-934.
[14] L.Y. Liang, D.S. Xue, Kinetics of cellulose hydrolysis by halostable cellulase from a marine Aspergillus niger at different salinities, Process Biochem. 63(2017) 163-168.
[15] D.S. Xue, L.Y. Liang, D.Q. Lin, C.J. Gong, S.J. Yao, Halostable catalytic properties of exoglucanase from a marine Aspergillus niger and secondary structure change caused by high salinities, Process Biochem. 58(2017) 85-91.
[16] A. Malik, M. Sakamoto, T. Ono, K. Kakii, Coaggregation between Acinetobacter johnsonii S35 and Microbacterium esteraromaticum strains isolated from sewage activated sludge, J. Biosci. Bioeng. 96(2003) 10-15.
[17] Z.H. Qiu, P.J. Shi, H.Y. Luo, Y.G. Bai, T.Z. Yuan, P.L. Yang, S.C. Liu, B. Yao, A xylanase with broad pH and temperature adaptability from Streptomyces megasporus DSM 41476, and its potential application in brewing industry, Enzym. Microb. Technol. 46(2010) 506-512.
[18] L.R.S. Moreira, M.C. Campos, P.H.V.M. Siqueira, L.P. Silva, C.A.O. Ricart, P.A. Martins, R.M.L. Queiroz, E.X.F. Filho, Two β-xylanases from Aspergillus terreus:Characterization and influence of phenolic compounds on xylanase activity, Fungal Genet. Biol. 60(2013) 46-52.
[19] D. Driss, J.G. Berrin, N. Juge, F. Bhiri, R. Ghorbel, S.E. Chaabouni, Functional characterization of Penicillium occitanis Pol6 and Penicillium funiculosum GH11 xylanases, Protein Expr. Purif. 90(2013) 195-201.
[20] P. Jampala, M. Preethi, S. Ramanujam, B.S. Harish, K.B. Uppuluri, V. Anbazhagan, Immobilization of levan-xylanase nanohybrid on an alginate bead improves xylanase stability at wide pH and temperature, Int. J. Biol. Macromol. 95(2017) 843-849.
[21] X. Huang, J. Lin, X. Ye, G. Wang, Molecular characterization of a thermophilic and salt-and alkaline-tolerant xylanase from Planococcus sp. SL4, a strain isolated from the sediment of a soda lake, J. Microbiol. Biotechnol. 25(2015) 662-671.
[22] W. Bai, Y. Xue, C. Zhou, Y. Ma, Cloning, expression and characterization of a novel salt-tolerant xylanase from Bacillus sp. SN5, Biotechnol. Lett. 34(2012) 2093-2099.
[23] S. Ali, A. Sayed, Regulation of cellulose biosynthesis in Aspergillus terreus, World J. Microbiol. Biotechnol. 8(1992) 73-75.
[24] O. Kotchoni, W. Gachomo, B. Omafuvbe, O. Shonukan, Purification and biochemical characterization of carboxymethyl cellulose (CMCase) from a catabolite repression insensitive mutant of Bacillus pumilus, Int. J. Agric. Biol. 8(2006) 286-292.
[25] Y. Sato, H. Fukuda, Y. Zhou, S. Mikami, Contribution of ethanol-tolerant xylanase G2 from Aspergillus oryzae on Japanese sake brewing, J. Biosci. Bioeng. 110(2010) 679-683.
[26] C.S. Goh, K.T. Lee, A visionary and conceptual macroalgae-based third-generation bioethanol (TGB) biorefinery in Sabah, Malaysia as an underlay for renewable and sustainable development, Renew. Sust. Energ. Rev. 14(2010) 842-848.
[27] A.G. Marangoni, Enzyme Kinetics:A Modern Approach, John Wiley & Sons, Inc., NJ, 2003146-150(ISBN:0-471-15985-9).

Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii

Thermostable ethanol tolerant xylanase from a cold-adapted marine species Acinetobacter johnsonii

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Recommended Articles

Metrics

Comments

[1]	Dongsheng Xue, Xuhao Zeng, Chunjie Gong, Dongqiang Lin, Shanjing Yao. A cold adapt and ethanol tolerant endoglucanase from a marine Bacillus subtilis [J]. Chin.J.Chem.Eng., 2018, 26(12): 2601-2606.
[2]	LI Yongquan, CHEN Shifei, CEN Peilin. Mutagenesis and Screening of High Yield Xylanase Production Strain of Aspergillus usamii by Microwave Irradiation [J]. , 2003, 11(5): 594-597.