Recombinant protein production in the filamentous fungus Trichoderma

doi:10.1016/j.cjche.2020.11.006

Chinese Journal of Chemical Engineering ›› 2021, Vol. 29 ›› Issue (2): 74-81.DOI: 10.1016/j.cjche.2020.11.006

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Recombinant protein production in the filamentous fungus Trichoderma

Huiling Wei, Mengyue Wu, Aili Fan, Haijia Su

Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, North Third Ring Road 15, Chaoyang District, Beijing 100029, China

Received:2020-07-22 Revised:2020-11-06 Online:2021-05-15 Published:2021-02-28
Contact: Aili Fan, Haijia Su
Supported by:
We are grateful to Prof. Shu-Ming Li from Philipps-Universität Marburg for critical reading of this manuscript. This work was supported by the National Key R&D Program of China (No. 2018YFA0902200], the National Natural Science Foundation of China (Nos. 21838001, 21525625, 31961133018 and 81803409), and the Fundamental Research Funds for the Central Universities (Nos. buctrc201810 and XK1802-8). We sincerely acknowledge the Mix-Up project, which has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 870294.

Recombinant protein production in the filamentous fungus Trichoderma

Huiling Wei, Mengyue Wu, Aili Fan, Haijia Su

Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, North Third Ring Road 15, Chaoyang District, Beijing 100029, China

通讯作者: Aili Fan, Haijia Su
基金资助:
We are grateful to Prof. Shu-Ming Li from Philipps-Universität Marburg for critical reading of this manuscript. This work was supported by the National Key R&D Program of China (No. 2018YFA0902200], the National Natural Science Foundation of China (Nos. 21838001, 21525625, 31961133018 and 81803409), and the Fundamental Research Funds for the Central Universities (Nos. buctrc201810 and XK1802-8). We sincerely acknowledge the Mix-Up project, which has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 870294.

Abstract

Abstract: Trichoderma is an ascomycete fungal genus widely distributed in the soils. Several species were selected, engineered and utilized for protein production for decades. The high extracellular secretion capability and eukaryotic post-translational modification machinery make Trichoderma spp. particularly interesting hosts. In this review, we summarized the recombinant proteins produced in Trichoderma since 2014, concerning their origins, hosts, promoters, terminators, signal peptides, yields and commonly used media. Meanwhile, strategies and merging trends in protein production and strain engineering are classified and summarized regarding codon optimization, promoter utilization, transcription factor regulation, post-translational modification and proteolytic degradation inhibition. With state-of-art biotechnologies and more available expression platforms, Trichoderma spp. could be more successful hosts to produce recombinant proteins as desired, i.e. better enzyme formula for efficient cellulose degradation or functional protein with high purity and yield.

Key words: Trichoderma, Heterologous expression, Protein, Catalysis, Biotechnology

摘要： Trichoderma is an ascomycete fungal genus widely distributed in the soils. Several species were selected, engineered and utilized for protein production for decades. The high extracellular secretion capability and eukaryotic post-translational modification machinery make Trichoderma spp. particularly interesting hosts. In this review, we summarized the recombinant proteins produced in Trichoderma since 2014, concerning their origins, hosts, promoters, terminators, signal peptides, yields and commonly used media. Meanwhile, strategies and merging trends in protein production and strain engineering are classified and summarized regarding codon optimization, promoter utilization, transcription factor regulation, post-translational modification and proteolytic degradation inhibition. With state-of-art biotechnologies and more available expression platforms, Trichoderma spp. could be more successful hosts to produce recombinant proteins as desired, i.e. better enzyme formula for efficient cellulose degradation or functional protein with high purity and yield.

关键词: Trichoderma, Heterologous expression, Protein, Catalysis, Biotechnology

Huiling Wei, Mengyue Wu, Aili Fan, Haijia Su. Recombinant protein production in the filamentous fungus Trichoderma[J]. Chinese Journal of Chemical Engineering, 2021, 29(2): 74-81.

Huiling Wei, Mengyue Wu, Aili Fan, Haijia Su. Recombinant protein production in the filamentous fungus Trichoderma[J]. 中国化学工程学报, 2021, 29(2): 74-81.

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Recombinant protein production in the filamentous fungus Trichoderma

Recombinant protein production in the filamentous fungus Trichoderma

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