Extraction of Theanine from Waste Liquid of Tea Polyphenol Production in Aqueous Two-phase Systems with Cationic and Anionic Surfactants

doi:10.1016/S1004-9541(13)60438-3

Abstract

Abstract: Extraction of theanine from waste liquid of tea polyphenol production was studied in aqueous surfactant two-phase system (ASTP) with cationic surfactant (CTAB) and anionic surfactant (SDS). Results indicate that the region of ASTP is narrow and there is only a two-phase region of cationic surfactant. The increase in concentrations of NaBr and Na₂SO₄ are beneficial to the formation of ASTP. Theanine concentration in the bottom phase increases with increasing concentration of theanine, whereas the partition coefficient and extraction rate only change a little when the concentration of theanine is above 0.2 g·L^-1. With the increase of SDS concentration, the phase ratio and the partition coefficient decrease, while the extraction efficiency of theanine increases and the concentration of theanine changes a little in the range from 2.4/7.5 to 2.8/7.2 for SDS/CTAB ratio. The temperature has a notable effect on the concentration of theanine in the bottom phase, partition coefficient and extraction rate of theanine. The increase of waste liquid decreases the phase ratio, increases the concentration and extraction rate of theanine in the bottom phase, since the protein and the saccharide enter the bottom phase with theanine.

Key words: aqueous two-phase system, surfactant, theanine, waste liquid of tea polyphenol production, extraction

摘要： Extraction of theanine from waste liquid of tea polyphenol production was studied in aqueous surfactant two-phase system (ASTP) with cationic surfactant (CTAB) and anionic surfactant (SDS). Results indicate that the region of ASTP is narrow and there is only a two-phase region of cationic surfactant. The increase in concentrations of NaBr and Na₂SO₄ are beneficial to the formation of ASTP. Theanine concentration in the bottom phase increases with increasing concentration of theanine, whereas the partition coefficient and extraction rate only change a little when the concentration of theanine is above 0.2 g·L^-1. With the increase of SDS concentration, the phase ratio and the partition coefficient decrease, while the extraction efficiency of theanine increases and the concentration of theanine changes a little in the range from 2.4/7.5 to 2.8/7.2 for SDS/CTAB ratio. The temperature has a notable effect on the concentration of theanine in the bottom phase, partition coefficient and extraction rate of theanine. The increase of waste liquid decreases the phase ratio, increases the concentration and extraction rate of theanine in the bottom phase, since the protein and the saccharide enter the bottom phase with theanine.

关键词: aqueous two-phase system, surfactant, theanine, waste liquid of tea polyphenol production, extraction

ZHANG Junwei, WANG Yan, PENG Qijun. Extraction of Theanine from Waste Liquid of Tea Polyphenol Production in Aqueous Two-phase Systems with Cationic and Anionic Surfactants[J]. Chin.J.Chem.Eng., 2013, 21(1): 31-36.

张军伟, 王艳, 彭奇均 . Extraction of Theanine from Waste Liquid of Tea Polyphenol Production in Aqueous Two-phase Systems with Cationic and Anionic Surfactants[J]. Chinese Journal of Chemical Engineering, 2013, 21(1): 31-36.

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