Ag-induced Efficient Immobilization of Papain on Silica Spheres

›› 2008, Vol. 16 ›› Issue (4): 612-619.

• BIOTECHNOLOGY & BIOENGINEERING • Previous Articles Next Articles

Ag-induced Efficient Immobilization of Papain on Silica Spheres

WANG Anming¹, WANG Hua¹, ZHOU Cheng¹, DU Zhiqiang¹, ZHU Shemin², SHEN Shubao¹

1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China;
2. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Materials Science and Engineering, Nanjing University of Technology, Nanjing 210009, China

Received:2007-06-26 Revised:2008-05-23 Online:2008-08-28 Published:2008-08-28
Supported by:
the National High Technology Research and Development Program of China(2006AA02Z211);the National Natural Science Foundation of China(20376034);the Natural Science Foundation of Jiangsu Province(BK2006181);the Foundation of Jiangsu Province for College Postgraduate Students in Innovation Engineering(2007)

Ag-induced Efficient Immobilization of Papain on Silica Spheres

王安明¹, 王华¹, 周成¹, 杜志强¹, 祝社民², 沈树宝¹

1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China;
2. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Materials Science and Engineering, Nanjing University of Technology, Nanjing 210009, China

通讯作者: SHEN Shubao, E-mail: zsbshen@hotmail.com
基金资助:
the National High Technology Research and Development Program of China(2006AA02Z211);the National Natural Science Foundation of China(20376034);the Natural Science Foundation of Jiangsu Province(BK2006181);the Foundation of Jiangsu Province for College Postgraduate Students in Innovation Engineering(2007)

Abstract

Abstract: Complexation and interaction between silver and amino group were applied to induce an efficient immobilization of papain on silica spheres. The silver nanoparticles were deposited on the silica spheres before papain was coupled to the silica spheres. The silica spheres with silver nanoparticles were characterized by high resolution transmission electron microscopy (HR-TEM), Fournier transform infrared spectroscopy (FT-IR), and UV-Vis scanning spectrometer. FT-IR spectrum was also used to characterize the immobilized and free papain. Effect of some factors on the activities of the immobilized papain was investigated. It was observed that the coupled yield and relative activity of the papain on Ag/SiO₂ were 1.17 and 1.86 times of those on the bare SiO₂, respectively. At an optimum concentration of silver, the observed activity of the immobilized papain was 2.1 times of that on the bare silica. In addition, the maximum specific activity of papain immobilized on Ag/SiO₂ was 819.9 U·mg^-1, which is slightly lower than that of the free papain, 906.2 U·mg^-1. Stability of the immobilized papain was also examined. The results indicate that the silver nanoparticles successfully induce a fine immobilization of papain.

Key words: silver nanoparticle, covalent immobilization, papain, electrostatic interaction

摘要： Complexation and interaction between silver and amino group were applied to induce an efficient immobilization of papain on silica spheres. The silver nanoparticles were deposited on the silica spheres before papain was coupled to the silica spheres. The silica spheres with silver nanoparticles were characterized by high resolution transmission electron microscopy (HR-TEM), Fournier transform infrared spectroscopy (FT-IR), and UV-Vis scanning spectrometer. FT-IR spectrum was also used to characterize the immobilized and free papain. Effect of some factors on the activities of the immobilized papain was investigated. It was observed that the coupled yield and relative activity of the papain on Ag/SiO₂ were 1.17 and 1.86 times of those on the bare SiO₂, respectively. At an optimum concentration of silver, the observed activity of the immobilized papain was 2.1 times of that on the bare silica. In addition, the maximum specific activity of papain immobilized on Ag/SiO₂ was 819.9 U·mg^-1, which is slightly lower than that of the free papain, 906.2 U·mg^-1. Stability of the immobilized papain was also examined. The results indicate that the silver nanoparticles successfully induce a fine immobilization of papain.

关键词: silver nanoparticle, covalent immobilization, papain, electrostatic interaction

WANG Anming, WANG Hua, ZHOU Cheng, DU Zhiqiang, ZHU Shemin, SHEN Shubao. Ag-induced Efficient Immobilization of Papain on Silica Spheres[J]. , 2008, 16(4): 612-619.

王安明, 王华, 周成, 杜志强, 祝社民, 沈树宝. Ag-induced Efficient Immobilization of Papain on Silica Spheres[J]. , 2008, 16(4): 612-619.

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Ag-induced Efficient Immobilization of Papain on Silica Spheres

Ag-induced Efficient Immobilization of Papain on Silica Spheres

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