Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

doi:10.1016/S1004-9541(13)60528-5

Chin.J.Chem.Eng. ›› 2013, Vol. 21 ›› Issue (6): 670-675.DOI: 10.1016/S1004-9541(13)60528-5

• BIOTECHNOLOGY AND BIOENGINEERING • Previous Articles Next Articles

Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

ZHOU Liya¹, WANG Cui¹, JIANG Yanjun^1,2, GAO Jing¹

1 School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China;
2 National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

Received:2012-06-25 Revised:2012-10-20 Online:2013-07-03 Published:2013-06-28
Contact: GAO Jing
Supported by:
Supported by the National Natural Science Foundation of China (21006020, 21276060, 21276062), the Natural Science Foundation of Hebei Province (B2010000035, B2011202095), the Science and Technology Research Key Project of Higher School in Hebei Province (ZD2010118)

Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

周丽亚¹, 王翠¹, 姜艳军^1,2, 高静¹

1 School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China;
2 National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

通讯作者: GAO Jing
基金资助:
Supported by the National Natural Science Foundation of China (21006020, 21276060, 21276062), the Natural Science Foundation of Hebei Province (B2010000035, B2011202095), the Science and Technology Research Key Project of Higher School in Hebei Province (ZD2010118)

Abstract

Abstract: A novel method was developed for papain immobilization through a biomimetic silicification process induced by papain. By incubating papain in a silica precursor solution, the papain-silica composite formed rapidly and papain was encapsulated. The encapsulation efficiency and the recovery activity were 82.60% and 83.09%, respectively. Compared with enzymes and biomolecules immobilized in biosilica matrix in the presence of additional silica-precipitating species, this papain encapsulation process, a biomimetic approach, realized high encapsulation efficiency by its autosilification activity under mild conditions (near-neutral pH and ambient temperature). Furthermore, the encapsulated papain exhibits enhanced thermal, pH, recycling and storage stabilities. Kinetic analysis showed that the biomimetic silica matrix did not significantly hinder the mass transport of substrate or the release of product.

Key words: papain, silica matrix, encapsulation, biosilicification, biomimetic

摘要： A novel method was developed for papain immobilization through a biomimetic silicification process induced by papain. By incubating papain in a silica precursor solution, the papain-silica composite formed rapidly and papain was encapsulated. The encapsulation efficiency and the recovery activity were 82.60% and 83.09%, respectively. Compared with enzymes and biomolecules immobilized in biosilica matrix in the presence of additional silica-precipitating species, this papain encapsulation process, a biomimetic approach, realized high encapsulation efficiency by its autosilification activity under mild conditions (near-neutral pH and ambient temperature). Furthermore, the encapsulated papain exhibits enhanced thermal, pH, recycling and storage stabilities. Kinetic analysis showed that the biomimetic silica matrix did not significantly hinder the mass transport of substrate or the release of product.

关键词: papain, silica matrix, encapsulation, biosilicification, biomimetic

ZHOU Liya, WANG Cui, JIANG Yanjun, GAO Jing . Immobilization of Papain in Biosilica Matrix and Its Catalytic Property[J]. Chin.J.Chem.Eng., 2013, 21(6): 670-675.

周丽亚, 王翠, 姜艳军, 高静. Immobilization of Papain in Biosilica Matrix and Its Catalytic Property[J]. Chinese Journal of Chemical Engineering, 2013, 21(6): 670-675.

References

1 Kröger, N., Deutzmann, R., Sumper, M., “Polycationic peptides from diatom biosilica that direct silica nanosphere formation”, Science, 286, 1129-1132 (1999).

2 Cha, J.N., Stucky, G.D., Morse, D.E., Deming, T.J., “Biomimetic synthesis of ordered silica structures mediated by block copolypeptides”, Nature, 403, 289-292 (2000).

3 Luckarift, H.R., Spain, J.C., Naik, R.R., Stone, M.O., “Enzyme immobilization in a biomimetic silica support”, Nat. Biotechnol., 22, 211-213 (2004).

4 Betancor, L., Luckarift, H.R., “Bioinspired enzyme encapsulation for biocatalysis”, Trends Biotechnol., 26, 566-572 (2008).

5 Choi, O., Kim, B.C., An, J.H., Min, K., Kim, Y.H., Um, Y., Oh, M.K., Sang, B.I., “A biosensor based on the self-entrapment of glucose oxidase within biomimetic silica nanoparticles induced by a fusion enzyme”, Enzyme Micro. Tech., 49, 441-445 (2011).

6 Das, S., Berke-Schlessel, D., Ji, H.F., McDonough, J., Wei, Y., “Enzymatic hydrolysis of biomass with recyclable use of cellobiase enzyme immobilized in sol-gel routed mesoporous silica”, J. Mol. Catal. B: Enzym., 70, 49-54 (2011).

7 Naik, R.R., Tomczak, M.M., Luckarift, H.R., Spain, J.C., Stone, M.O., “Entrapment of enzymes and nanoparticles using biomimetically synthesized silica”, Chem. Commun., (15), 1684-1685 (2004).

8 Luckarift, H.R., Johnson, G.R., Spain, J.C., “Silica-immobilized enzyme reactors; application to cholinesterase-inhibition studies”, J. Chromatogr. B, 843, 310-316 (2006).

9 Luckarift, H.R., Ku, B.S., Dordick, J.S., Spain, J.C., “Silica-immobilized enzymes for multi-step synthesis in microfluidic devices”, Biotechnol. Bioeng., 98, 701-705 (2007).

10 Swartz, J.D., Miller, S.A., Wright, D., “Rapid production of nitrilase containing silica nanoparticles offers an effective and reusable biocatalyst for synthetic nitrile hydrolysis”, Org. Process Res. Dev., 13, 584-589 (2009).

11 Zamora, P., Narváez, A., Domínguez, E., “Enzyme-modified nanoparticles using biomimetically synthesized silica”, Bioelectrochemistry, 76, 100-106 (2009).

12 Cha, J.N., Shimizu, K., Zhou, Y., Christiansen, S.C., Chmelka, B.F., Stucky, G.D., Morse, D.E., “Silicatein filaments and subunits from a marine sponge direct the polymerization of silica and silicones in vitro”, Proc. Natl. Acad. Sci. USA, 96, 361-365 (1999).

13 Zhang, Y.F., Wu, H., Li, J., Li, L., Jiang, Y.J., Jiang, Z.Y., “Protamine-templated biomimetic hybrid capsules: efficient and stable carrier for enzyme encapsulation”, Chem. Mater., 20, 1041-1048 (2008).

14 Sano, K.I., Minamisawa, T., Shiba, K., “Autonomous silica encapsulation and sustained release of anticancer protein”, Langmuir, 26, 2231-2234 (2010).

15 Berne, C., Betancor, L., Luckarift, H.R., Spain, J.C., “Application of a microfluidic reactor for screening cancer prodrug activation using silica-immobilized nitrobenzene nitroreductase”, Biomacromolecules, 7, 2631-2636 (2006).

16 Madadlou, A., Iacopino, D., Sheehan, D., Emam-Djomeh, Z., Mousavi, M.E., “Enhanced thermal and ultrasonic stability of a fungal protease encapsulated within biomimetically generated silicate nanospheres”, BBA-Gen. Subjects, 1800, 459-465 (2010).

17 Roth, K.M., Zhou, Y., Yang, W., Morse, D.E., “Bifunctional small molecules are biomimetic catalysts for silica synthesis at neutral pH”, J. Am. Chem. Soc., 127, 325-330 (2005).

18 Marner II, W.D., Shaikh, A.S., Muller, S.J., Keasling, J.D., “Enzyme immobilization via silaffin-mediated autoencapsulation in a biosilica support”, Biotechnol. Progr., 25, 417-423 (2009).

19 Luckarift, H.R., Dickerson, M.B., Sandhage, K.H., Spain, J.C., “Rapid, room-temperature synthesis of antibacterial bionanocomposites of lysozyme with amorphous silica or titania”, Small, 2, 640-643 (2006).

20 Bassindale, A.R., Taylor, P.G., Abbate, V., Brandstadt, K.F., “Simple and mild preparation of silica-enzyme composites from silicic acid solution”, J. Mater. Chem., 19, 7606-7609 (2009).

21 Smith, G.P., Baustian, K.J., Ackerson, C.J., Feldheim, D.L., “Metal oxide formation by serine and cysteine proteases”, J. Mater. Chem., 19, 8299-8306 (2009).

22 Frampton, M., Vawda, A., Fletcher, J., Zelisko, P.M., “Enzyme-mediated sol-gel processing of alkoxysilanes”, Chem. Commun., (43), 5544-5546 (2008).

23 Buisson, P., Rassy, H.E., Maury, S., Pierre, A.C., “Biocatalytic gelation of silica in the presence of a lipase”, J. Sol-Gel Sci. Techn., 27, 373-379 (2003).

24 Lazo-Wasem, E.A., “Standardization of papain activity: Report of a collaborative study”, J. Pharm. Sci., 55, 723-725 (1966).

25 Shimizu, K., Cha, J., Stucky, G.D., Morse, D.E., “Silicatein α: cathepsin L-like protein in sponge biosilica”, Proc. Natl. Acad. Sci. USA, 95, 6234-6238 (1998).

26 Brunner, E., Lutz, K., Sumper, M., “Biomimetic synthesis of silica nanospheres depends on the aggregation and phase separation of polyamines in aqueous solution”, Phys. Chem. Chem. Phys., 6, 854-857 (2004).

27 Ramanathan, M., Luckarift, H.R., Sarsenova, A., Wild, J.R., Ramanculov, E. K., Olsen, E. V., Simonian, A.L., “Lysozyme-mediated formation of protein-silica nano-composites for biosensing applications”, Colloid. Surface. B, 73, 58-64 (2009).

28 Miller, S.A., Hong, E.D., Wright, D., “Rapid and efficient enzyme encapsulation in a dendrimer silica nanocomposite”, Macromol. Biosci., 6, 839-845 (2006).

29 Lai, J.K., Chuang, T.H., Jan, J.S., Wang, S.S., “Efficient and stable enzyme immobilization in a block copolypeptide vesicle-templated biomimetic silica support”, Colloid. Surface. B, 80, 51-58 (2010).

30 Li, F.Y., Xing, Y.J., Ding, X., “Immobilization of papain on cotton fabric by sol-gel method”, Enzyme. Microb. Tech., 40, 1692-1697 (2007).

31 Wang, A., Wang, H., Zhou, C., Du, Z., Zhu, S., Shen, S., “Ag-induced efficient immobilization of papain on silica spheres”, Chin. J. Chem. Eng., 16, 612-619 (2008).

32 Jiang, Y.J., Sun, Q.Y., Jiang, Z.Y., Zhang, L., Li, J., Li, L., Sun, X.H., “The improved stability of enzyme encapsulated in biomimetic titania particles”, Mat. Sci. Eng. C, 29, 328-334 (2009).

33 Avnir, D., Coradin, T., Lev, O., Livage, J., “Recent bio-applications of sol-gel materials”, J. Mater. Chem., 16, 1013-1030 (2006).

34 Cardoso, M.B., Luckarift, H.R., Urban, V.S., O'Neill, H., Johnson, G.R., “Protein localization in silica nanospheres derived via biomimetic mineralization”, Adv. Funct. Mater., 20, 3031-3038 (2010).

[1]	Chaoqun Wu, Xun Liu, Fujun Yao, Xin Yang, Yan Wang, Wenyuan Hu. Crystalline-magnetism action in biomimetic mineralization of calcium carbonate [J]. Chinese Journal of Chemical Engineering, 2023, 59(7): 146-152.
[2]	Zhenfu Wang, Jie Gao, Qinghong Shi, Xiaoyan Dong, Yan Sun. Facile purification and immobilization of organophosphorus hydrolase on protein-inorganic hybrid phosphate nanosheets [J]. Chinese Journal of Chemical Engineering, 2023, 56(4): 119-125.
[3]	Hongwei Guo, Linyuan Chen, Xueying Zhang, Huanhao Chen, Yan Shao. Silicalite-1 zeolite encapsulated Fe nanocatalyst for Fenton-like degradation of methylene blue [J]. Chinese Journal of Chemical Engineering, 2023, 53(1): 251-259.
[4]	Wenjun Zhang, Wenshu Ge, Min Li, Shuangqing Li, Minqiang Jiang, Xiujuan Zhang, Gaohong He. Short review on liquid membrane technology and their applications in biochemical engineering [J]. Chinese Journal of Chemical Engineering, 2022, 49(9): 21-33.
[5]	Bo Zhang, Hongwen Chen, Liming Jiang, Youqing Shen, Dan Zhao, Zhuxian Zhou. A breathing A4 paper by in situ growth of green metal–organic frameworks for air freshening and cleaning [J]. Chinese Journal of Chemical Engineering, 2022, 52(12): 95-102.
[6]	Junwei Wu, Hongjia Zhou, Jingyi Zhou, Xiao Zhu, Bowen Zhang, Shasha Feng, Zhaoxiang Zhong, Lingxue Kong, Weihong Xing. Meltblown fabric vs nanofiber membrane, which is better for fabricating personal protective equipments [J]. Chinese Journal of Chemical Engineering, 2021, 36(8): 1-9.
[7]	Chengpan Li, Jing Liu, Qiang Wu, Xiangyu Chen, Weiping Ding. Alginate core-shell microcapsule reduces the DMSO addition-induced osmotic damage to cells by inhibiting cellular blebs [J]. Chinese Journal of Chemical Engineering, 2021, 33(5): 249-255.
[8]	Qirui Tian, Weiqing Zhou, Qiong Cai, Guanghui Ma, Guoping Lian. Concepts, processing, and recent developments in encapsulating essential oils [J]. Chinese Journal of Chemical Engineering, 2021, 29(2): 255-271.
[9]	Xiao Liang, Qing Li, Zhiyuan Shi, Shaowei Bai, Quanshun Li. Immobilization of urease in metal-organic frameworks via biomimetic mineralization and its application in urea degradation [J]. Chinese Journal of Chemical Engineering, 2020, 28(8): 2173-2180.
[10]	Shouwu Yu, Shujuan Xiao, Zewen Zhao, Xiaowen Huo, Junfu Wei. Microencapsulated ammonium polyphosphate by polyurethane with segment of dipentaerythritol and its application in flame retardant polypropylene [J]. Chinese Journal of Chemical Engineering, 2019, 27(7): 1735-1743.
[11]	Ting Zhang, Minmin Chen, Yu Zhang, Yi Wang. Microencapsulation of stearic acid with polymethylmethacrylate using iron (III) chloride as photo-initiator for thermal energy storage [J]. , 2017, 25(10): 1524-1532.
[12]	Ting Zhang, Minmin Chen, Yu Zhang, Yi Wang. Microencapsulation of stearic acid with polymethylmethacrylate using iron (III) chloride as photo-initiator for thermal energy storage [J]. , 2017, 25(10): 1524-1532.
[13]	Daohong Xia, Shengjuan Jiang, Lantao Li, Yuzhi Xiang, Lijun Zhu. The biomimetic catalytic synthesis of acetal compounds using β-cyclodextrin as catalyst [J]. Chin.J.Chem.Eng., 2016, 24(1): 146-150.
[14]	SHE Yuanbin, WANG Weijie, LI Guojun. Oxidation of p/o-Cresols to p/o-Hydroxybenzaldehydes Catalyzed by Metalloporphyrins with Molecular Oxygen [J]. Chin.J.Chem.Eng., 2012, 20(2): 262-266.
[15]	XIA Fei, JIN Heyang, ZHAO Yaping, GUO Xinqiu. Supercritical Antisolvent-based Technology for Preparation of Vitamin D₃ Proliposome and Its Characteristics [J]. , 2011, 19(6): 1039-1046.

Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

Immobilization of Papain in Biosilica Matrix and Its Catalytic Property

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments