Alginate core-shell microcapsule reduces the DMSO addition-induced osmotic damage to cells by inhibiting cellular blebs

doi:10.1016/j.cjche.2020.10.019

Chinese Journal of Chemical Engineering ›› 2021, Vol. 33 ›› Issue (5): 249-255.DOI: 10.1016/j.cjche.2020.10.019

• Biotechnology and Bioengineering • Previous Articles Next Articles

Alginate core-shell microcapsule reduces the DMSO addition-induced osmotic damage to cells by inhibiting cellular blebs

Chengpan Li^1,2, Jing Liu³, Qiang Wu⁴, Xiangyu Chen⁵, Weiping Ding^1,2

1 Center for Biomedical Engineering, University of Science and Technology of China, Hefei 230027, China;
2 Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, China;
3 School of Biology, Food and Environment Engineering, Hefei University, Hefei 230601, China;
4 Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230027, China;
5 Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China

Received:2020-07-01 Revised:2020-09-02 Online:2021-08-19 Published:2021-05-28
Contact: Weiping Ding
Supported by:
This work was partially supported by the National Natural Science Foundation of China (81571768). We would like to thank Department of Precision Machinery and Precision Instrumentation at the University of Science and Technology of China for assistance.

Alginate core-shell microcapsule reduces the DMSO addition-induced osmotic damage to cells by inhibiting cellular blebs

Chengpan Li^1,2, Jing Liu³, Qiang Wu⁴, Xiangyu Chen⁵, Weiping Ding^1,2

1 Center for Biomedical Engineering, University of Science and Technology of China, Hefei 230027, China;
2 Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, China;
3 School of Biology, Food and Environment Engineering, Hefei University, Hefei 230601, China;
4 Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230027, China;
5 Institute of Intelligent Machines, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China

通讯作者: Weiping Ding
基金资助:
This work was partially supported by the National Natural Science Foundation of China (81571768). We would like to thank Department of Precision Machinery and Precision Instrumentation at the University of Science and Technology of China for assistance.

Abstract

Abstract: In cryopreservation, the addition of cryoprotectant can change the intra- and extra-cellular osmotic pressure, affect the cell morphology, and induce blebs on the plasma membrane. In this study, the blebs of cells microencapsulated in the alginate microsphere induced by osmotic shock were studied, and the effects of microencapsulation on bleb size and cell viability were determined. Firstly, a coaxial co-flow focusing device was applied to generate cell-laden microcapsules using alginate hydrogel in this paper. Then, cellular blebs induced by DMSO with various concentrations under microencapsulation were compared with that when non-encapsulated, and the dynamic process of cellular bleb was investigated. Finally, the qualitative relationship between bleb size and cell viability in the presence of DMSO was built, and thus the effects of microencapsulation on bleb size and viability were evaluated. The results show that the bleb size is smaller and the cell viability is higher, and cell microencapsulation can significantly inhibit the excessively large blebs generated on the cell membrane and reduce the osmotic damage to cells when loading cryoprotectant and then to improve cell viability during cryopreservation. This work can provide insights for optimizing cryoprotectant-loading protocols, offer a new avenue to study cell blebbing, and advance future research on cryopreservation of rare cells and biomaterials.

Key words: Cell blebbing, Microencapsulation, Osmotic shock, DMSO, Cell viability

摘要： In cryopreservation, the addition of cryoprotectant can change the intra- and extra-cellular osmotic pressure, affect the cell morphology, and induce blebs on the plasma membrane. In this study, the blebs of cells microencapsulated in the alginate microsphere induced by osmotic shock were studied, and the effects of microencapsulation on bleb size and cell viability were determined. Firstly, a coaxial co-flow focusing device was applied to generate cell-laden microcapsules using alginate hydrogel in this paper. Then, cellular blebs induced by DMSO with various concentrations under microencapsulation were compared with that when non-encapsulated, and the dynamic process of cellular bleb was investigated. Finally, the qualitative relationship between bleb size and cell viability in the presence of DMSO was built, and thus the effects of microencapsulation on bleb size and viability were evaluated. The results show that the bleb size is smaller and the cell viability is higher, and cell microencapsulation can significantly inhibit the excessively large blebs generated on the cell membrane and reduce the osmotic damage to cells when loading cryoprotectant and then to improve cell viability during cryopreservation. This work can provide insights for optimizing cryoprotectant-loading protocols, offer a new avenue to study cell blebbing, and advance future research on cryopreservation of rare cells and biomaterials.

关键词: Cell blebbing, Microencapsulation, Osmotic shock, DMSO, Cell viability

Chengpan Li, Jing Liu, Qiang Wu, Xiangyu Chen, Weiping Ding. Alginate core-shell microcapsule reduces the DMSO addition-induced osmotic damage to cells by inhibiting cellular blebs[J]. Chinese Journal of Chemical Engineering, 2021, 33(5): 249-255.

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Alginate core-shell microcapsule reduces the DMSO addition-induced osmotic damage to cells by inhibiting cellular blebs

Alginate core-shell microcapsule reduces the DMSO addition-induced osmotic damage to cells by inhibiting cellular blebs

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