Biodegradation of Aniline by a Newly Isolated Delftia sp. XYJ6

Abstract

Abstract: A promising gram-negative bacterial strain for the biodegradation of aniline as the sole carbon, nitrogen and energy sources was successfully isolated and identified as Delftia sp. XYJ6. The optimal temperature and pH for both the growth of Delftia sp. XYJ6 and the biodegradation of aniline were 30℃ and 7.0, respectively. Initial aniline of 2000 mg·L^-1 could be completely removed by the strain at 22 h, which showed that Delftia sp. XYJ6 had a strong ability in the biodegradation of aniline. It indicated that aniline was firstly converted to catechol catalyzed by aniline dioxygenase as a first product, which was then further biodegraded to cis,cis-muconic acid catalyzed by the catechol 1,2-dioxygenase of Delftia sp. XYJ6 as a second product. Cis,cis-muconic acid could also be further biodegraded to other small compound again. The pathway for the biodegradation of aniline by Delftia sp. XYJ6 was not previously reported.

Key words: aniline, Delftia sp., biodegradation, pathway

摘要： A promising gram-negative bacterial strain for the biodegradation of aniline as the sole carbon, nitrogen and energy sources was successfully isolated and identified as Delftia sp. XYJ6. The optimal temperature and pH for both the growth of Delftia sp. XYJ6 and the biodegradation of aniline were 30℃ and 7.0, respectively. Initial aniline of 2000 mg·L^-1 could be completely removed by the strain at 22 h, which showed that Delftia sp. XYJ6 had a strong ability in the biodegradation of aniline. It indicated that aniline was firstly converted to catechol catalyzed by aniline dioxygenase as a first product, which was then further biodegraded to cis,cis-muconic acid catalyzed by the catechol 1,2-dioxygenase of Delftia sp. XYJ6 as a second product. Cis,cis-muconic acid could also be further biodegraded to other small compound again. The pathway for the biodegradation of aniline by Delftia sp. XYJ6 was not previously reported.

关键词: aniline, Delftia sp., biodegradation, pathway

XIAO Chengbin, NING Jun, YAN Hai, SUN Xudong, HU Jiye. Biodegradation of Aniline by a Newly Isolated Delftia sp. XYJ6[J]. , 2009, 17(3): 500-505.

肖诚斌, 宁君, 闫海, 孙旭东, 胡继业. Biodegradation of Aniline by a Newly Isolated Delftia sp. XYJ6[J]. , 2009, 17(3): 500-505.

References

1 O’Neill, F.J., Bromley-Challenor K.C.A., Greenwood R.J., Knapp, J.S., “Bacterial growth an aniline:implications for the biotreatment of industrial wastewater”, Wat. Res., 34, 4397-4409(2000).
2 Kearney, P.C., Kaufmann, D.D., Herbicides:Chemistry, Degradation and Mode of Action, Marcel Dekker, New York(1975).
3 Emtiazi, G., Satarii, M., Mazaherion, F., “The utilization of aniline, chlorinated aniline, and aniline blue as the only source of nitrogen by fungi in water”, Wat. Res., 35, 1219-1224(2001).
4 Gheewala, S.H., Annachhatre, A.P., “Biodegradation of aniline”, Wat. Sci. Tech., 36, 53-63(1997).
5 Lyons, C.D., Katz, S., Bartha, R., “Persistence and mutagenic potential of herbicide-derived aniline residues in pond water”, Bull. Environ. Contam. Toxicol., 35, 696-703(1985).
6 Khan, M.F., Wu, X.H., Kaphalia, B.S., Boor, P.J., Ansari, G.A.S., “Nitrotyrosine formation in splenic toxicity of aniline”, Toxicology, 194, 95-102(2003).
7 Wang, L., Barrington, S., Kim, J.W., “Biodegradation of pentyl amine and aniline from petrochemical wastewater”, J. Environ. Manage., 83, 191-197(2007).
8 Urata, M., Uchida, E., Nojiri, H., Omori, T., Obo, R., Miyaura, N., Ouchiyama, N., “Genes involved in aniline degradation by Delftia acidovorans strain 7N and its distribution in the natural environment”, Biosci. Biotechnol. Biochem., 68(12), 2457-2465(2004).
9 Wen, J.P., Li, H.M., Bai, J., Jiang, Y., “Biodegradation of 4-chlorophenol by Candida albicans PDY-07 under anaerobic conditions”, Chin. J. Chem. Eng., 14(6), 790-795(2006).
10 Jiang, Y., Ren, N.Q., Cai, X., Wu, D., Qiao, L.Y., Lin, S., “Biodegradation of phenol and 4-chlorophenol by the mutant strain CTM 2”, Chin. J. Chem. Eng., 16(5), 796-800(2008).
11 Konopka, A., Knight, D., Turco, R.F., “Characterization of a Pseudomonas sp. capable of aniline degradation in the presence of secondary carbon sources”, Appl. Environ. Microbiol., 48, 491-496(1989).
12 Zeyer, J., Wasserfallen, A., Timmis, K.N., “Microbial mineralization of ring-substituted anilines through an ortho-cleavage pathway”, Appl. Environ. Microbiol., 50, 447-453(1985).
13 Parales, R.E., Ontl, T.A., Gibson, D.T., “Cloning and sequence analysis of a catechol 2,3-dioxygenase gene from the nitrobenzene-degrading strain Comamonas sp. JS765”, J. Ind. Microbiol. Biotechnol., 19, 385-391(1997).
14 Schukat, B., Janke, D., Krebs, D., Fritsche, W., “Cometabolic degradation of 2and 3-chloroaniline because of glucose metabolism by Rhodococcus sp. AN117”, Curr. Microbiol., 9, 81-86(1983).
15 Aoki, K., Ohtsuka, K., Shinke, R., Nishira, H., “Rapid biodegradation of aniline by Frateuria species ANA-18”, Agri. Biol. Chem., 48, 865-872(1984).
16 Bachofer, R., Lingens, F., Schafer, W., “Conversion of aniline into pyrocatechol by a Nocardia sp.:Incorporation of oxygen-18”, FEBS Lett., 50, 288-290(1975).
17 Aoki, K., Ohtsuka, K., Shinke, R., Nishira, H., “Rapid Biodegradation of aniline by Frateuria species ANA-18 and its aniline metabolism”, Agric. Biol. Chem., 48(4), 865-872(1984).
18 Kahng, H.Y., Kukor, J.J., Oh, K.H., “Characterization of strain HY99, a novel microorganism capable of aerobic and anaerobic degradation of aniline”, FEMs Microbiol. Lett., 190, 215-221(2000).
19 Liu, Z., Yang, H., Huang, Z., Zhou, P., Liu, S.J., “Degradation of aniline by newly isolated, extremely aniline-tolerant Delftia sp. AN3”, Appl. Microbiol. Biotechnol., 58, 679-682(2002).
20 Kahng, H.Y., Kukor, J.J., Oh, K.H.J., “Physiological and phylogenetic analysis of Burkholderia sp. HY1 capable of aniline degradation”, J. Microbiol. Biotechnol., 10, 643-650(2000).
21 Zhang, T., Zhang, J.L., Liu, S.J., Liu, Z.P., “A novel and complete gene cluster involved in the degradation of aniline by Delftia sp. AN3”, J. Environ. Sci., 20, 717-724(2008).
22 Sheludchenko, M.S., Kolomytseva, M.P., “Degradation of aniline by Delftia tsuruhatensis 14S in batch and continuous processes”, Appl. Biochem. Microbiol., 41(5), 465-468(2005).
23 Radianingtyas, H., Robinson, G.K., Bull, A.T., “Characterization of a soil-derived bacterial consortium degrading 4-chloroaniline”, Microbiology, 149, 3279-3287(2003).
24 Bi, H.K., Huang, Y., Wu, B., “Isolation, identification and characterization of aniline-degrading strain GXA7”, J. Guangxi University, 28, 309-313(2003).(in Chinese)

[1]	Xun Tao, Fan Zhou, Xinlei Yu, Songling Guo, Yunfei Gao, Lu Ding, Guangsuo Yu, Zhenghua Dai, Fuchen Wang. Effect of carbon dioxide on oxy-fuel combustion of hydrogen sulfide: An experimental and kinetic modeling [J]. Chinese Journal of Chemical Engineering, 2023, 59(7): 105-117.
[2]	Iltaf Khan, Chunjuan Wang, Shoaib Khan, Jinyin Chen, Aftab Khan, Sayyar Ali Shah, Aihua Yuan, Sohail Khan, Mehwish K. Butt, Humaira Asghar. Bio-capped and green synthesis of ZnO/g-C₃N₄ nanocomposites and its improved antibiotic and photocatalytic activities: An exceptional approach towards environmental remediation [J]. Chinese Journal of Chemical Engineering, 2023, 56(4): 215-224.
[3]	Chao Zhang, Youzhi Liu, Weizhou Jiao, Hongyan Shen, Xigang Yuan, Shengkun Jia. An optimization method for enhancement of gas–liquid mass transfer in a bubble column reactor based on the entropy generation extremum principle [J]. Chinese Journal of Chemical Engineering, 2023, 53(1): 83-88.
[4]	Xiaobo Ruan, Sheng Zhang, Wei Song, Jia Liu, Xiulai Chen, Liming Liu, Jing Wu. Efficient synthesis of tyrosol from L-tyrosine via heterologous Ehrlich pathway in Escherichia coli [J]. Chinese Journal of Chemical Engineering, 2022, 47(7): 18-30.
[5]	Xiangjun Li, Shujun Li, Xiaoping Wang, Muhammad Asif Nawaz, Dianhua Liu. Polyoxymethylene dimethyl ethers synthesis from methanol and formaldehyde solution over one-pot synthesized spherical mesoporous sulfated zirconia [J]. Chinese Journal of Chemical Engineering, 2022, 46(6): 161-172.
[6]	Iman Farirzadeh, Majid Riahi Samani, Davood Toghraie. Lead removal from aqueous medium using fruit peels and polyaniline composites in aqueous and non-aqueous solvents in the presence of polyethylene glycol [J]. Chinese Journal of Chemical Engineering, 2022, 44(4): 253-259.
[7]	Wanyuan Wang, Chengxin Wen, Daoyuan Zheng, Chunhu Li, Junjie Bian, Xinbo Wang. Simultaneous degradation of RhB and reduction of Cr(VI) by MIL-53(Fe)/Polyaniline (PANI) with the mediation of organic acid [J]. Chinese Journal of Chemical Engineering, 2022, 42(2): 55-63.
[8]	Yipeng Zheng, Zhennan Cao, Zheng Zhou, Gaodong Yang, Zhibing Zhang. Kinetics study of the N-formylation of aniline with DMF catalyzed by temperature-controlled Brønsted ionic liquids [J]. Chinese Journal of Chemical Engineering, 2022, 52(12): 154-160.
[9]	Xinxiao Sun, Xianglai Li, Xiaolin Shen, Jia Wang, Qipeng Yuan. Recent advances in microbial production of phenolic compounds [J]. Chinese Journal of Chemical Engineering, 2021, 29(2): 54-61.
[10]	Yinfeng Zhang, Fang Fang, Yongjie Chen, Min Li, Li Li, Wenyue Li, Jinfeng Zhang. Hollow mesoporous polyaniline nanoparticles with high drug payload and robust photothermal capability for cancer combination therapy [J]. Chinese Journal of Chemical Engineering, 2021, 38(10): 221-228.
[11]	Qun Wang, Lanhui Jiang, Chengran Fang, Hongzhi Mao, Haifeng Zhuang. Transformation of phthalic acid diesters in an anaerobic/anoxic/oxic leachate treatment process [J]. Chinese Journal of Chemical Engineering, 2020, 28(1): 249-253.
[12]	Xiaoqiang Jia, Yun He, Lei Huang, Dawei Jiang, Wenyu Lu. n-Hexadecane and pyrene biodegradation and metabolization by Rhodococcus sp. T1 isolated from oil contaminated soil [J]. Chin.J.Chem.Eng., 2019, 27(2): 411-417.
[13]	Yuejiao Qin, Shuai Luo, Shuo Geng, Weizhou Jiao, Youzhi Liu. Degradation and mineralization of aniline by O₃/Fenton process enhanced using high-gravity technology [J]. Chin.J.Chem.Eng., 2018, 26(7): 1444-1450.
[14]	Mohammad Mehdi Amin, Bijan Bina, Afshin Ebrahimi, Zeynab Yavari, Farzaneh Mohammadi, Somayeh Rahimi. The occurrence, fate, and distribution of natural and synthetic hormones in different types of wastewater treatment plants in Iran [J]. Chin.J.Chem.Eng., 2018, 26(5): 1132-1139.
[15]	Hong Guo, Xin Li, Ziyi Wang, Bao'an Li, Jixiao Wang, Shichang Wang. Thermal conductivity of PVDF/PANI-nanofiber composite membrane aligned in an electric field [J]. Chin.J.Chem.Eng., 2018, 26(5): 1213-1218.