SCI和EI收录∣中国化工学会会刊

›› 2009, Vol. 17 ›› Issue (6): 1032-1037.

• BIOTECHNOLOGY AND BIOENGINEERING • Previous Articles     Next Articles

Analysis of Sucrose Esters with Long Acyl Chain by Coupling of HPLC-ELSD with ESI-MS System

ZHU Jinli1, TANG Yanfeng1, LI Jianhua1, ZHANG Shufen2   

  1. 1. School of Chemistry and Chemical Engineering, Nantong University, Nantong 226007, China;
    2. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China
  • Received:2009-05-01 Revised:2009-09-07 Online:2009-12-28 Published:2009-12-28
  • Supported by:
    Supported by the National Natural Science Foundation of China (20906052);the Science Foundation of Nantong City Municipality (K2007011,K2008023);the Science Foundation of Nantong University (08R08);the University Science Research Project of Jiangsu Province (09KJB530008)

Analysis of Sucrose Esters with Long Acyl Chain by Coupling of HPLC-ELSD with ESI-MS System

朱金丽1, 汤艳峰1, 李建华1, 张淑芬2   

  1. 1. School of Chemistry and Chemical Engineering, Nantong University, Nantong 226007, China;
    2. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China
  • 通讯作者: TANG Yanfeng,E-mail:tangyf@ntu.edu.cn
  • 基金资助:
    Supported by the National Natural Science Foundation of China (20906052);the Science Foundation of Nantong City Municipality (K2007011,K2008023);the Science Foundation of Nantong University (08R08);the University Science Research Project of Jiangsu Province (09KJB530008)

Abstract: The analysis of sucrose esters with long acyl chain by improved high performance liquid chromatographic method with evaporative light scattering detection(HPLC-ELSD) and electrospray ionization mass spectrum(ESI-MS) is investigated.The improved HPLC-ELSD method for the separation and quantitation of commercial and synthesized sucrose esters is described.Samples are analyzed by means of a reversed-phase(RP) HPLC using a Hypersil C8 column(250 mm×4.6 mm,5 μm particle size) with methanol-tetrahydrofuran(volume ratio of 90:10) and water under gradient condition as the mobile phase,in which the flow rate is 1.0 ml·min-1 and the column temperature is set at 40℃.This procedure provides a complete separation and determination of monoester,diester,triester and higher esters with different acyl chain lengths in each fraction by a single run,in combination with the ESI-MS technology.With this method,it is possible to determine the approximate compositions of mono-to polyesters in one analysis and quantitate pure positional isomers precisely using an external standard method.It is found that the method of ESI-MS coupling with HPLC system for the analysis of sucrose esters is straight forward,rapid and inexpensive,and can be readily applied in synthesis,purification and structure studies.

Key words: sucrose esters, long acyl chain, analysis, high performance liquid chromatographic method with evaporative light scattering detection, electrospray ionization mass spectrum

摘要: The analysis of sucrose esters with long acyl chain by improved high performance liquid chromatographic method with evaporative light scattering detection(HPLC-ELSD) and electrospray ionization mass spectrum(ESI-MS) is investigated.The improved HPLC-ELSD method for the separation and quantitation of commercial and synthesized sucrose esters is described.Samples are analyzed by means of a reversed-phase(RP) HPLC using a Hypersil C8 column(250 mm×4.6 mm,5 μm particle size) with methanol-tetrahydrofuran(volume ratio of 90:10) and water under gradient condition as the mobile phase,in which the flow rate is 1.0 ml·min-1 and the column temperature is set at 40℃.This procedure provides a complete separation and determination of monoester,diester,triester and higher esters with different acyl chain lengths in each fraction by a single run,in combination with the ESI-MS technology.With this method,it is possible to determine the approximate compositions of mono-to polyesters in one analysis and quantitate pure positional isomers precisely using an external standard method.It is found that the method of ESI-MS coupling with HPLC system for the analysis of sucrose esters is straight forward,rapid and inexpensive,and can be readily applied in synthesis,purification and structure studies.

关键词: sucrose esters, long acyl chain, analysis, high performance liquid chromatographic method with evaporative light scattering detection, electrospray ionization mass spectrum