Analysis of Sucrose Esters with Long Acyl Chain by Coupling of HPLC-ELSD with ESI-MS System

›› 2009, Vol. 17 ›› Issue (6): 1032-1037.

• BIOTECHNOLOGY AND BIOENGINEERING • Previous Articles Next Articles

Analysis of Sucrose Esters with Long Acyl Chain by Coupling of HPLC-ELSD with ESI-MS System

ZHU Jinli¹, TANG Yanfeng¹, LI Jianhua¹, ZHANG Shufen²

1. School of Chemistry and Chemical Engineering, Nantong University, Nantong 226007, China;
2. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China

Received:2009-05-01 Revised:2009-09-07 Online:2009-12-28 Published:2009-12-28
Supported by:
Supported by the National Natural Science Foundation of China (20906052);the Science Foundation of Nantong City Municipality (K2007011,K2008023);the Science Foundation of Nantong University (08R08);the University Science Research Project of Jiangsu Province (09KJB530008)

Analysis of Sucrose Esters with Long Acyl Chain by Coupling of HPLC-ELSD with ESI-MS System

朱金丽¹, 汤艳峰¹, 李建华¹, 张淑芬²

1. School of Chemistry and Chemical Engineering, Nantong University, Nantong 226007, China;
2. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China

通讯作者: TANG Yanfeng,E-mail:tangyf@ntu.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China (20906052);the Science Foundation of Nantong City Municipality (K2007011,K2008023);the Science Foundation of Nantong University (08R08);the University Science Research Project of Jiangsu Province (09KJB530008)

Abstract

Abstract: The analysis of sucrose esters with long acyl chain by improved high performance liquid chromatographic method with evaporative light scattering detection(HPLC-ELSD) and electrospray ionization mass spectrum(ESI-MS) is investigated.The improved HPLC-ELSD method for the separation and quantitation of commercial and synthesized sucrose esters is described.Samples are analyzed by means of a reversed-phase(RP) HPLC using a Hypersil C8 column(250 mm×4.6 mm,5 μm particle size) with methanol-tetrahydrofuran(volume ratio of 90:10) and water under gradient condition as the mobile phase,in which the flow rate is 1.0 ml·min^-1 and the column temperature is set at 40℃.This procedure provides a complete separation and determination of monoester,diester,triester and higher esters with different acyl chain lengths in each fraction by a single run,in combination with the ESI-MS technology.With this method,it is possible to determine the approximate compositions of mono-to polyesters in one analysis and quantitate pure positional isomers precisely using an external standard method.It is found that the method of ESI-MS coupling with HPLC system for the analysis of sucrose esters is straight forward,rapid and inexpensive,and can be readily applied in synthesis,purification and structure studies.

Key words: sucrose esters, long acyl chain, analysis, high performance liquid chromatographic method with evaporative light scattering detection, electrospray ionization mass spectrum

摘要： The analysis of sucrose esters with long acyl chain by improved high performance liquid chromatographic method with evaporative light scattering detection(HPLC-ELSD) and electrospray ionization mass spectrum(ESI-MS) is investigated.The improved HPLC-ELSD method for the separation and quantitation of commercial and synthesized sucrose esters is described.Samples are analyzed by means of a reversed-phase(RP) HPLC using a Hypersil C8 column(250 mm×4.6 mm,5 μm particle size) with methanol-tetrahydrofuran(volume ratio of 90:10) and water under gradient condition as the mobile phase,in which the flow rate is 1.0 ml·min^-1 and the column temperature is set at 40℃.This procedure provides a complete separation and determination of monoester,diester,triester and higher esters with different acyl chain lengths in each fraction by a single run,in combination with the ESI-MS technology.With this method,it is possible to determine the approximate compositions of mono-to polyesters in one analysis and quantitate pure positional isomers precisely using an external standard method.It is found that the method of ESI-MS coupling with HPLC system for the analysis of sucrose esters is straight forward,rapid and inexpensive,and can be readily applied in synthesis,purification and structure studies.

关键词: sucrose esters, long acyl chain, analysis, high performance liquid chromatographic method with evaporative light scattering detection, electrospray ionization mass spectrum

ZHU Jinli, TANG Yanfeng, LI Jianhua, ZHANG Shufen. Analysis of Sucrose Esters with Long Acyl Chain by Coupling of HPLC-ELSD with ESI-MS System[J]. , 2009, 17(6): 1032-1037.

朱金丽, 汤艳峰, 李建华, 张淑芬. Analysis of Sucrose Esters with Long Acyl Chain by Coupling of HPLC-ELSD with ESI-MS System[J]. , 2009, 17(6): 1032-1037.

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Analysis of Sucrose Esters with Long Acyl Chain by Coupling of HPLC-ELSD with ESI-MS System

Analysis of Sucrose Esters with Long Acyl Chain by Coupling of HPLC-ELSD with ESI-MS System

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