Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125

›› 2010, Vol. 18 ›› Issue (5): 870-875.

• RESEARCH NOTES • Previous Articles Next Articles

Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125

LI Zheng^1,2, DENG Li¹, LU Jike^1,3, GUO Xiaolei¹, YANG Zixin¹, TAN Tianwei¹

1. Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China;
2. School of Textiles, Tianjin Polytechnic University, Tianjin 300160, China;
3. Bioengineering Department, Zhengzhou University, Zhengzhou 450001, China

Received:2009-10-20 Revised:2010-06-13 Online:2010-10-28 Published:2010-10-28
Supported by:
Supported by the National High Technology Research and Development Program of China (2006AA020101, 2007AA10Z360,2009AA03Z232);Key Projects in the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period (2008BA163B07)

Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125

李政^1,2, 邓利¹, 鲁吉珂^1,3, 郭小雷¹, 杨自信¹, 谭天伟¹

1. Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China;
2. School of Textiles, Tianjin Polytechnic University, Tianjin 300160, China;
3. Bioengineering Department, Zhengzhou University, Zhengzhou 450001, China

通讯作者: TAN Tianwei,E-mail:twtan@mail.buct.edu.cn
基金资助:
Supported by the National High Technology Research and Development Program of China (2006AA020101, 2007AA10Z360,2009AA03Z232);Key Projects in the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period (2008BA163B07)

Abstract

Abstract: The non-edible crude rice bran oil was extracted from white rice bran, and then was catalyzed by immobilized lipase for biodiesel production in this study. The effects of water content, oil/methanol molar ratio, temperature, enzyme amount, solvent,number of methanol added times and two-step methanolysis by using Candida sp. 99-125 as catalyst were investigated. The optimal conditions for processing 1 g rice bran oil were:0.2 g immobilized lipase, 2 ml n-hexane as solvent, 20% water based on the rice bran oil mass, temperature of 40℃ and two-step addition of methanol. As a result, the fatty acid methyl esters yield was 87.4%. The immobilized lipase was proved to be stable when it was used repeatedly for 7 cycles.

Key words: immobilized lipase, transesterification, rice bran oil, methanolysis, optimization

摘要： The non-edible crude rice bran oil was extracted from white rice bran, and then was catalyzed by immobilized lipase for biodiesel production in this study. The effects of water content, oil/methanol molar ratio, temperature, enzyme amount, solvent,number of methanol added times and two-step methanolysis by using Candida sp. 99-125 as catalyst were investigated. The optimal conditions for processing 1 g rice bran oil were:0.2 g immobilized lipase, 2 ml n-hexane as solvent, 20% water based on the rice bran oil mass, temperature of 40℃ and two-step addition of methanol. As a result, the fatty acid methyl esters yield was 87.4%. The immobilized lipase was proved to be stable when it was used repeatedly for 7 cycles.

关键词: immobilized lipase, transesterification, rice bran oil, methanolysis, optimization

LI Zheng, DENG Li, LU Jike, GUO Xiaolei, YANG Zixin, TAN Tianwei. Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125[J]. , 2010, 18(5): 870-875.

李政, 邓利, 鲁吉珂, 郭小雷, 杨自信, 谭天伟. Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125[J]. , 2010, 18(5): 870-875.

References

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Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125

Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125

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